Effect of Diode Laser on Bacteria Beyond the Apex in Relation to the Size of the Apical Preparation – An In-Vitro Study
Introduction: Microorganisms causing periapical infection are usually difficult to eradicate after conventional endodontic treatment or even in retreatment resulting in poor outcomes. So the purpose of the study was to assess whether disinfection of root canal with laser had any effect on bacter...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2016-05-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/7791/17759_CE(EK)_F(AK)_PF1(EkGH)_PFA(AK)_PF2(PAG).pdf |
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| author | Sistla Venkata Jyotsna Raja Vatsavai Chakradhar Raju Jaya Prakash Patil Thakur Veerandar Singh Neha Bhutani Shekar Kamishetty Kiran Ghatole |
| author_facet | Sistla Venkata Jyotsna Raja Vatsavai Chakradhar Raju Jaya Prakash Patil Thakur Veerandar Singh Neha Bhutani Shekar Kamishetty Kiran Ghatole |
| author_sort | Sistla Venkata Jyotsna |
| collection | DOAJ |
| description | Introduction: Microorganisms causing periapical infection
are usually difficult to eradicate after conventional endodontic
treatment or even in retreatment resulting in poor outcomes. So
the purpose of the study was to assess whether disinfection of
root canal with laser had any effect on bacteria in the periapex
region.
Aim: The aim of the present study was to evaluate the effects
of a diode laser when activated in root canals with varying
apical diameters, on the bacteria present beyond the apex of
the teeth.
Materials and Methods: Total 30 intact single rooted teeth were
taken and decoronated to standardize the root to a length of
12mm. They were divided into three groups depending on last
file size used for instrumentation at apex i.e., size 30, 40 and 50
respectively. The samples were then mounted on test tubes such
that roots of teeth were in contact with fresh broth of Escherichia
coli (ATCC 25922) and left for incubation. Later a diode laser
(Ezlase 940, Biolase) was used for disinfection of root canals of
the samples. Following this the bacterial inoculums from each
test tube were cultured and CFU were obtained from which the
mean log values were obtained. Statistical analysis was done
using Kruskal Wallis ANOVA test to compare mean CFU in three
groups. Mann-Whitney U test with Bonferroni correction was
used to compare inter-group differences.
Results: There was statistically significant difference in mean
log values of CFU in all the three study groups. Inter-group
comparisons showed that, Group A had significantly lower
mean CFUs than Group B and C respectively.
Conclusion: The study showed that intracanal irradiation with
diode laser had an effect on the bacteria present beyond the
apex, and it was influenced by the size of the apical preparation
i.e., smaller apical size led to a greater reduction in the bacterial
count. |
| first_indexed | 2024-12-10T14:16:59Z |
| format | Article |
| id | doaj.art-984848e7bdb54e2b82b98a22a30c3ce3 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-10T14:16:59Z |
| publishDate | 2016-05-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-984848e7bdb54e2b82b98a22a30c3ce32022-12-22T01:45:19ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-05-01105ZC63ZC6510.7860/JCDR/2016/17759.7791Effect of Diode Laser on Bacteria Beyond the Apex in Relation to the Size of the Apical Preparation – An In-Vitro StudySistla Venkata Jyotsna0Raja Vatsavai Chakradhar Raju1Jaya Prakash Patil2Thakur Veerandar Singh3Neha Bhutani4Shekar Kamishetty5Kiran Ghatole6Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, India.Professor and Head, Department of Conservative Dentistry and Endodontics, Sri Balaji Dental College, Moinabad, India. Professor and Head, Department of Conservative Dentistry and Endodontics, PMNM Dental College, Bagalkot, India.Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, India.Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, India. Professor, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, India.Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, India.Introduction: Microorganisms causing periapical infection are usually difficult to eradicate after conventional endodontic treatment or even in retreatment resulting in poor outcomes. So the purpose of the study was to assess whether disinfection of root canal with laser had any effect on bacteria in the periapex region. Aim: The aim of the present study was to evaluate the effects of a diode laser when activated in root canals with varying apical diameters, on the bacteria present beyond the apex of the teeth. Materials and Methods: Total 30 intact single rooted teeth were taken and decoronated to standardize the root to a length of 12mm. They were divided into three groups depending on last file size used for instrumentation at apex i.e., size 30, 40 and 50 respectively. The samples were then mounted on test tubes such that roots of teeth were in contact with fresh broth of Escherichia coli (ATCC 25922) and left for incubation. Later a diode laser (Ezlase 940, Biolase) was used for disinfection of root canals of the samples. Following this the bacterial inoculums from each test tube were cultured and CFU were obtained from which the mean log values were obtained. Statistical analysis was done using Kruskal Wallis ANOVA test to compare mean CFU in three groups. Mann-Whitney U test with Bonferroni correction was used to compare inter-group differences. Results: There was statistically significant difference in mean log values of CFU in all the three study groups. Inter-group comparisons showed that, Group A had significantly lower mean CFUs than Group B and C respectively. Conclusion: The study showed that intracanal irradiation with diode laser had an effect on the bacteria present beyond the apex, and it was influenced by the size of the apical preparation i.e., smaller apical size led to a greater reduction in the bacterial count.https://jcdr.net/articles/PDF/7791/17759_CE(EK)_F(AK)_PF1(EkGH)_PFA(AK)_PF2(PAG).pdfapical diameterapical periodontitisescherichia coliroot canal disinfection |
| spellingShingle | Sistla Venkata Jyotsna Raja Vatsavai Chakradhar Raju Jaya Prakash Patil Thakur Veerandar Singh Neha Bhutani Shekar Kamishetty Kiran Ghatole Effect of Diode Laser on Bacteria Beyond the Apex in Relation to the Size of the Apical Preparation – An In-Vitro Study Journal of Clinical and Diagnostic Research apical diameter apical periodontitis escherichia coli root canal disinfection |
| title | Effect of Diode Laser on Bacteria Beyond the Apex in Relation to the Size of the Apical Preparation – An In-Vitro Study |
| title_full | Effect of Diode Laser on Bacteria Beyond the Apex in Relation to the Size of the Apical Preparation – An In-Vitro Study |
| title_fullStr | Effect of Diode Laser on Bacteria Beyond the Apex in Relation to the Size of the Apical Preparation – An In-Vitro Study |
| title_full_unstemmed | Effect of Diode Laser on Bacteria Beyond the Apex in Relation to the Size of the Apical Preparation – An In-Vitro Study |
| title_short | Effect of Diode Laser on Bacteria Beyond the Apex in Relation to the Size of the Apical Preparation – An In-Vitro Study |
| title_sort | effect of diode laser on bacteria beyond the apex in relation to the size of the apical preparation an in vitro study |
| topic | apical diameter apical periodontitis escherichia coli root canal disinfection |
| url | https://jcdr.net/articles/PDF/7791/17759_CE(EK)_F(AK)_PF1(EkGH)_PFA(AK)_PF2(PAG).pdf |
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