IN-VITRO ANTIFUNGAL POTENTIAL OF dsRNA MOLECULES ON FUSARIUM ACTIN RELATED PROTEIN 2/3, DNA POLYMERASE DELTA SUBUNIT AND ADENYLASE CYCLASE ESSENTIAL GENES ON COLLAR ROT AND WILT PATHOGENS OF PASSIONFRUIT.
BACKGROUND: In Uganda two fungal diseases have become economically important; Fusarium wilt which is caused by.Fusarium oxysporum. f.sp passiflorae (Fop) and collar rot caused by Fusarium solani (Fs). The aim of this study was to determine antifungal activity of synthetic dsRNA constructed to sil...
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| Format: | Article |
| Language: | English |
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Student's Journal of Health Research
2023-06-01
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| Series: | Student's Journal of Health Research Africa |
| Subjects: | |
| Online Access: | https://sjhresearchafrica.org/index.php/public-html/article/view/363 |
| _version_ | 1797791324175335424 |
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| author | Florence Nassimbwa Enock Matovu Andrew Kiggundu Charles Changa Francis Mumbanza John Adriko |
| author_facet | Florence Nassimbwa Enock Matovu Andrew Kiggundu Charles Changa Francis Mumbanza John Adriko |
| author_sort | Florence Nassimbwa |
| collection | DOAJ |
| description |
BACKGROUND:
In Uganda two fungal diseases have become economically important; Fusarium wilt which is caused by.Fusarium oxysporum. f.sp passiflorae (Fop) and collar rot caused by Fusarium solani (Fs). The aim of this study was to determine antifungal activity of synthetic dsRNA constructed to silence essential genes; Actin Related Protein 2/3 (D6), DNA Polymerase delta subunit (J6), and Adenylase cyclase (K6), in Fusarium from banana on Fusarium sp from passionfruit.
METHODS:
In this study, pathogenic samples which were previously isolated from 3 districts in Central Uganda i.e. Wakiso, Mpigi, and Mukono were tested to determine and explore RNAi as an anti-fungal control strategy for spore germination. Sample materials obtained were subjected to DNA extraction from pure mycelia and PCR was performed. Antifungal dsRNA assays were run to test the efficiency relying on the reduction in a number of fungal colonies on PDA.
RESULTS:
Previous results showed that wilting was associated with one specific species of Fusarium oxysporum and collar rot with one species of Fusarium solani. Anti-fungal assay tests in this study included PCRs targeting the Fusarium actin related protein 2/3 (D6), DNA polymerase delta subunit (J6) as well as adenylase cyclase (K6) results showed that % spore germination inhibition on Fop ranged from 61.9-93.9% and Fs ranged from -69-87.3%.
CONCLUSION:
These results show that an environmentally friendly control measure for the above diseases is possible through the development of resistant plants which express dsRNA for a specified gene_ Fusarium Actin Related Protein 2/3.
RECOMMENDATION:
RNAi would be a promising target gene for RNAi –mediated resistance in passion fruit against the target fungal diseases.
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| first_indexed | 2024-03-13T02:17:09Z |
| format | Article |
| id | doaj.art-984cf0c7252646feaafaaa6d5819fa32 |
| institution | Directory Open Access Journal |
| issn | 2709-9997 |
| language | English |
| last_indexed | 2024-03-13T02:17:09Z |
| publishDate | 2023-06-01 |
| publisher | Student's Journal of Health Research |
| record_format | Article |
| series | Student's Journal of Health Research Africa |
| spelling | doaj.art-984cf0c7252646feaafaaa6d5819fa322023-06-30T11:43:37ZengStudent's Journal of Health ResearchStudent's Journal of Health Research Africa2709-99972023-06-014610.51168/sjhrafrica.v4i6.363 IN-VITRO ANTIFUNGAL POTENTIAL OF dsRNA MOLECULES ON FUSARIUM ACTIN RELATED PROTEIN 2/3, DNA POLYMERASE DELTA SUBUNIT AND ADENYLASE CYCLASE ESSENTIAL GENES ON COLLAR ROT AND WILT PATHOGENS OF PASSIONFRUIT.Florence Nassimbwa0Enock Matovu1Andrew Kiggundu2Charles Changa3Francis Mumbanza4John Adriko5School of Biosecurity, Biotechnical and Laboratory Sciences Makerere University. P. O. Box 7062 Kampala, Uganda.School of Biosecurity, Biotechnical and Laboratory Sciences Makerere University. P. O. Box 7062 Kampala, Uganda.National Biotechnology Research Center/ Tissue Culture, National Agricultural Research Laboratories. P. O. Box 7065 Kampala, Uganda.National Biotechnology Research Center/ Tissue Culture, National Agricultural Research Laboratories. P. O. Box 7065 Kampala, Uganda.National Biotechnology Research Center/ Tissue Culture, National Agricultural Research Laboratories. P. O. Box 7065 Kampala, Uganda.National Biotechnology Research Center/ Tissue Culture, National Agricultural Research Laboratories. P. O. Box 7065 Kampala, Uganda. BACKGROUND: In Uganda two fungal diseases have become economically important; Fusarium wilt which is caused by.Fusarium oxysporum. f.sp passiflorae (Fop) and collar rot caused by Fusarium solani (Fs). The aim of this study was to determine antifungal activity of synthetic dsRNA constructed to silence essential genes; Actin Related Protein 2/3 (D6), DNA Polymerase delta subunit (J6), and Adenylase cyclase (K6), in Fusarium from banana on Fusarium sp from passionfruit. METHODS: In this study, pathogenic samples which were previously isolated from 3 districts in Central Uganda i.e. Wakiso, Mpigi, and Mukono were tested to determine and explore RNAi as an anti-fungal control strategy for spore germination. Sample materials obtained were subjected to DNA extraction from pure mycelia and PCR was performed. Antifungal dsRNA assays were run to test the efficiency relying on the reduction in a number of fungal colonies on PDA. RESULTS: Previous results showed that wilting was associated with one specific species of Fusarium oxysporum and collar rot with one species of Fusarium solani. Anti-fungal assay tests in this study included PCRs targeting the Fusarium actin related protein 2/3 (D6), DNA polymerase delta subunit (J6) as well as adenylase cyclase (K6) results showed that % spore germination inhibition on Fop ranged from 61.9-93.9% and Fs ranged from -69-87.3%. CONCLUSION: These results show that an environmentally friendly control measure for the above diseases is possible through the development of resistant plants which express dsRNA for a specified gene_ Fusarium Actin Related Protein 2/3. RECOMMENDATION: RNAi would be a promising target gene for RNAi –mediated resistance in passion fruit against the target fungal diseases. https://sjhresearchafrica.org/index.php/public-html/article/view/363Antifungal potentialactin related proteinadenlylase cyclasedsRNAPCRpolymerase delta subunit |
| spellingShingle | Florence Nassimbwa Enock Matovu Andrew Kiggundu Charles Changa Francis Mumbanza John Adriko IN-VITRO ANTIFUNGAL POTENTIAL OF dsRNA MOLECULES ON FUSARIUM ACTIN RELATED PROTEIN 2/3, DNA POLYMERASE DELTA SUBUNIT AND ADENYLASE CYCLASE ESSENTIAL GENES ON COLLAR ROT AND WILT PATHOGENS OF PASSIONFRUIT. Student's Journal of Health Research Africa Antifungal potential actin related protein adenlylase cyclase dsRNA PCR polymerase delta subunit |
| title | IN-VITRO ANTIFUNGAL POTENTIAL OF dsRNA MOLECULES ON FUSARIUM ACTIN RELATED PROTEIN 2/3, DNA POLYMERASE DELTA SUBUNIT AND ADENYLASE CYCLASE ESSENTIAL GENES ON COLLAR ROT AND WILT PATHOGENS OF PASSIONFRUIT. |
| title_full | IN-VITRO ANTIFUNGAL POTENTIAL OF dsRNA MOLECULES ON FUSARIUM ACTIN RELATED PROTEIN 2/3, DNA POLYMERASE DELTA SUBUNIT AND ADENYLASE CYCLASE ESSENTIAL GENES ON COLLAR ROT AND WILT PATHOGENS OF PASSIONFRUIT. |
| title_fullStr | IN-VITRO ANTIFUNGAL POTENTIAL OF dsRNA MOLECULES ON FUSARIUM ACTIN RELATED PROTEIN 2/3, DNA POLYMERASE DELTA SUBUNIT AND ADENYLASE CYCLASE ESSENTIAL GENES ON COLLAR ROT AND WILT PATHOGENS OF PASSIONFRUIT. |
| title_full_unstemmed | IN-VITRO ANTIFUNGAL POTENTIAL OF dsRNA MOLECULES ON FUSARIUM ACTIN RELATED PROTEIN 2/3, DNA POLYMERASE DELTA SUBUNIT AND ADENYLASE CYCLASE ESSENTIAL GENES ON COLLAR ROT AND WILT PATHOGENS OF PASSIONFRUIT. |
| title_short | IN-VITRO ANTIFUNGAL POTENTIAL OF dsRNA MOLECULES ON FUSARIUM ACTIN RELATED PROTEIN 2/3, DNA POLYMERASE DELTA SUBUNIT AND ADENYLASE CYCLASE ESSENTIAL GENES ON COLLAR ROT AND WILT PATHOGENS OF PASSIONFRUIT. |
| title_sort | in vitro antifungal potential of dsrna molecules on fusarium actin related protein 2 3 dna polymerase delta subunit and adenylase cyclase essential genes on collar rot and wilt pathogens of passionfruit |
| topic | Antifungal potential actin related protein adenlylase cyclase dsRNA PCR polymerase delta subunit |
| url | https://sjhresearchafrica.org/index.php/public-html/article/view/363 |
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