The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease.
BACKGROUND: Strongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC3289599?pdf=render |
| _version_ | 1818689070152286208 |
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| author | Antonio Marcilla Antonio Marcilla Gagan Garg Dolores Bernal Shoba Ranganathan Javier Forment Javier Ortiz Carla Muñoz-Antolí M Victoria Dominguez Laia Pedrola Juan Martinez-Blanch Javier Sotillo Maria Trelis Rafael Toledo J Guillermo Esteban |
| author_facet | Antonio Marcilla Antonio Marcilla Gagan Garg Dolores Bernal Shoba Ranganathan Javier Forment Javier Ortiz Carla Muñoz-Antolí M Victoria Dominguez Laia Pedrola Juan Martinez-Blanch Javier Sotillo Maria Trelis Rafael Toledo J Guillermo Esteban |
| author_sort | Antonio Marcilla |
| collection | DOAJ |
| description | BACKGROUND: Strongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its hosts. Next generation sequencing technologies now provide unique opportunities to rapidly address these questions. PRINCIPAL FINDINGS: Here we present the first transcriptome of the third larval stage of S. stercoralis using 454 sequencing coupled with semi-automated bioinformatic analyses. 253,266 raw sequence reads were assembled into 11,250 contiguous sequences, most of which were novel. 8037 putative proteins were characterized based on homology, gene ontology and/or biochemical pathways. Comparison of the transcriptome of S. strongyloides with those of other nematodes, including S. ratti, revealed similarities in transcription of molecules inferred to have key roles in parasite-host interactions. Enzymatic proteins, like kinases and proteases, were abundant. 1213 putative excretory/secretory proteins were compiled using a new pipeline which included non-classical secretory proteins. Potential drug targets were also identified. CONCLUSIONS: Overall, the present dataset should provide a solid foundation for future fundamental genomic, proteomic and metabolomic explorations of S. stercoralis, as well as a basis for applied outcomes, such as the development of novel methods of intervention against this neglected parasite. |
| first_indexed | 2024-12-17T12:04:15Z |
| format | Article |
| id | doaj.art-9850d753c1144872a2a566f46d7e5a6e |
| institution | Directory Open Access Journal |
| issn | 1935-2735 |
| language | English |
| last_indexed | 2024-12-17T12:04:15Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-9850d753c1144872a2a566f46d7e5a6e2022-12-21T21:49:42ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27352012-01-0162e151310.1371/journal.pntd.0001513The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease.Antonio MarcillaAntonio MarcillaGagan GargDolores BernalShoba RanganathanJavier FormentJavier OrtizCarla Muñoz-AntolíM Victoria DominguezLaia PedrolaJuan Martinez-BlanchJavier SotilloMaria TrelisRafael ToledoJ Guillermo EstebanBACKGROUND: Strongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its hosts. Next generation sequencing technologies now provide unique opportunities to rapidly address these questions. PRINCIPAL FINDINGS: Here we present the first transcriptome of the third larval stage of S. stercoralis using 454 sequencing coupled with semi-automated bioinformatic analyses. 253,266 raw sequence reads were assembled into 11,250 contiguous sequences, most of which were novel. 8037 putative proteins were characterized based on homology, gene ontology and/or biochemical pathways. Comparison of the transcriptome of S. strongyloides with those of other nematodes, including S. ratti, revealed similarities in transcription of molecules inferred to have key roles in parasite-host interactions. Enzymatic proteins, like kinases and proteases, were abundant. 1213 putative excretory/secretory proteins were compiled using a new pipeline which included non-classical secretory proteins. Potential drug targets were also identified. CONCLUSIONS: Overall, the present dataset should provide a solid foundation for future fundamental genomic, proteomic and metabolomic explorations of S. stercoralis, as well as a basis for applied outcomes, such as the development of novel methods of intervention against this neglected parasite.http://europepmc.org/articles/PMC3289599?pdf=render |
| spellingShingle | Antonio Marcilla Antonio Marcilla Gagan Garg Dolores Bernal Shoba Ranganathan Javier Forment Javier Ortiz Carla Muñoz-Antolí M Victoria Dominguez Laia Pedrola Juan Martinez-Blanch Javier Sotillo Maria Trelis Rafael Toledo J Guillermo Esteban The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease. PLoS Neglected Tropical Diseases |
| title | The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease. |
| title_full | The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease. |
| title_fullStr | The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease. |
| title_full_unstemmed | The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease. |
| title_short | The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease. |
| title_sort | transcriptome analysis of strongyloides stercoralis l3i larvae reveals targets for intervention in a neglected disease |
| url | http://europepmc.org/articles/PMC3289599?pdf=render |
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