Impact of Perinatal Coexposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive Organs
Emerging evidence has indicated the involvement of extrahypothalamic Kisspeptin and GnRHR in reproductive function. In this study, we evaluate if maternal exposure to the pesticide chlorpyrifos (CPF) and/or a high-fat diet (HFD) has an impact on the expression of Kisspeptin and GnRHR in the reproduc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/9/789 |
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| author | Marwa Lahimer Narimane Djekkoun Sophian Tricotteaux-Zarqaoui Aurélie Corona Isabelle Lafosse Habib Ben Ali Mounir Ajina Véronique Bach Moncef Benkhalifa Hafida Khorsi-Cauet |
| author_facet | Marwa Lahimer Narimane Djekkoun Sophian Tricotteaux-Zarqaoui Aurélie Corona Isabelle Lafosse Habib Ben Ali Mounir Ajina Véronique Bach Moncef Benkhalifa Hafida Khorsi-Cauet |
| author_sort | Marwa Lahimer |
| collection | DOAJ |
| description | Emerging evidence has indicated the involvement of extrahypothalamic Kisspeptin and GnRHR in reproductive function. In this study, we evaluate if maternal exposure to the pesticide chlorpyrifos (CPF) and/or a high-fat diet (HFD) has an impact on the expression of Kisspeptin and GnRHR in the reproductive organs of rats’ offspring. A total of 16 pregnant rats are divided into four groups: a control group (<i>n</i> = 4), CPF group (4 rats exposed daily to 1/mg/kg/day), HFD group (4 rats randomly fed a 5.25 kcal/g HFD), and coexposed group (4 rats exposed to CPF and HDF). At postnatal development postnatal day (PND) 60, male and female offspring were sacrificed. The reproductive organs (ovary and testis) were removed, and histological and immunohistological analysis and in silico quantification (TissueGnostics software 6.0.1.102, TissueFAXS, HistoQuest) were applied to investigate the impact of different treatments on Kisspeptin and GnRHR expression in reproductive organs. The main outcomes of the study showed a significant decrease in rat offspring’s body weight in the CPF group from PND30 and PND60 (<i>p</i> < 0.05 and <i>p</i> < 0.01, respectively). Histological analysis showed a significant increase in the atretic follicle and abnormal testis structure with germ cell desquamation in the CPF-exposed group. The immunodetection quantification of protein shows a significant decrease in GnRHR and Kisspeptin in the HFD and CPF exposed groups, respectively, in testis rat offspring. Perinatal exposure to CPF and HFD exposure affect the reproduction function of rat offspring. |
| first_indexed | 2024-03-10T21:53:50Z |
| format | Article |
| id | doaj.art-9851b07ec5204b119ea761e1f012b2a5 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-10T21:53:50Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-9851b07ec5204b119ea761e1f012b2a52023-11-19T13:15:12ZengMDPI AGToxics2305-63042023-09-0111978910.3390/toxics11090789Impact of Perinatal Coexposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive OrgansMarwa Lahimer0Narimane Djekkoun1Sophian Tricotteaux-Zarqaoui2Aurélie Corona3Isabelle Lafosse4Habib Ben Ali5Mounir Ajina6Véronique Bach7Moncef Benkhalifa8Hafida Khorsi-Cauet9PERITOX-(UMR-I 01), UPJV/INERIS, UPJV, CURS, Chemin du Thil, 80025 Amiens, FrancePERITOX-(UMR-I 01), UPJV/INERIS, UPJV, CURS, Chemin du Thil, 80025 Amiens, FrancePERITOX-(UMR-I 01), UPJV/INERIS, UPJV, CURS, Chemin du Thil, 80025 Amiens, FrancePERITOX-(UMR-I 01), UPJV/INERIS, UPJV, CURS, Chemin du Thil, 80025 Amiens, FranceMP3CV—UPJV—UR 7517, Jules Verne University of Picardie, 80025 Amiens, FranceLaboratory Histology Embryology, Faculty of Medicine Sousse, University of Sousse, Sousse 4000, TunisiaExercise Physiology and Physiopathology: From Integrated to Molecular “Biology, Medicine and 9 Health” (Code: LR19ES09), Sousse 4002, TunisiaPERITOX-(UMR-I 01), UPJV/INERIS, UPJV, CURS, Chemin du Thil, 80025 Amiens, FrancePERITOX-(UMR-I 01), UPJV/INERIS, UPJV, CURS, Chemin du Thil, 80025 Amiens, FrancePERITOX-(UMR-I 01), UPJV/INERIS, UPJV, CURS, Chemin du Thil, 80025 Amiens, FranceEmerging evidence has indicated the involvement of extrahypothalamic Kisspeptin and GnRHR in reproductive function. In this study, we evaluate if maternal exposure to the pesticide chlorpyrifos (CPF) and/or a high-fat diet (HFD) has an impact on the expression of Kisspeptin and GnRHR in the reproductive organs of rats’ offspring. A total of 16 pregnant rats are divided into four groups: a control group (<i>n</i> = 4), CPF group (4 rats exposed daily to 1/mg/kg/day), HFD group (4 rats randomly fed a 5.25 kcal/g HFD), and coexposed group (4 rats exposed to CPF and HDF). At postnatal development postnatal day (PND) 60, male and female offspring were sacrificed. The reproductive organs (ovary and testis) were removed, and histological and immunohistological analysis and in silico quantification (TissueGnostics software 6.0.1.102, TissueFAXS, HistoQuest) were applied to investigate the impact of different treatments on Kisspeptin and GnRHR expression in reproductive organs. The main outcomes of the study showed a significant decrease in rat offspring’s body weight in the CPF group from PND30 and PND60 (<i>p</i> < 0.05 and <i>p</i> < 0.01, respectively). Histological analysis showed a significant increase in the atretic follicle and abnormal testis structure with germ cell desquamation in the CPF-exposed group. The immunodetection quantification of protein shows a significant decrease in GnRHR and Kisspeptin in the HFD and CPF exposed groups, respectively, in testis rat offspring. Perinatal exposure to CPF and HFD exposure affect the reproduction function of rat offspring.https://www.mdpi.com/2305-6304/11/9/789chlorpyrifoshigh-fat dietKisspeptinGnRHRovarytestis |
| spellingShingle | Marwa Lahimer Narimane Djekkoun Sophian Tricotteaux-Zarqaoui Aurélie Corona Isabelle Lafosse Habib Ben Ali Mounir Ajina Véronique Bach Moncef Benkhalifa Hafida Khorsi-Cauet Impact of Perinatal Coexposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive Organs Toxics chlorpyrifos high-fat diet Kisspeptin GnRHR ovary testis |
| title | Impact of Perinatal Coexposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive Organs |
| title_full | Impact of Perinatal Coexposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive Organs |
| title_fullStr | Impact of Perinatal Coexposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive Organs |
| title_full_unstemmed | Impact of Perinatal Coexposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive Organs |
| title_short | Impact of Perinatal Coexposure to Chlorpyrifos and a High-Fat Diet on Kisspeptin and GnRHR Presence and Reproductive Organs |
| title_sort | impact of perinatal coexposure to chlorpyrifos and a high fat diet on kisspeptin and gnrhr presence and reproductive organs |
| topic | chlorpyrifos high-fat diet Kisspeptin GnRHR ovary testis |
| url | https://www.mdpi.com/2305-6304/11/9/789 |
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