Atomic force microscopy analysis of the Acinetobacter baumannii bacteriophage AP22 lytic cycle.
BACKGROUND: Acinetobacter baumannii is known for its ability to develop resistance to the major groups of antibiotics, form biofilms, and survive for long periods in hospital environments. The prevalence of infections caused by multidrug-resistant A. baumannii is a significant problem for the modern...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3469531?pdf=render |
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| author | Evgeniy V Dubrovin Anastasia V Popova Sergey V Kraevskiy Sergei G Ignatov Tatyana E Ignatyuk Igor V Yaminsky Nikolay V Volozhantsev |
| author_facet | Evgeniy V Dubrovin Anastasia V Popova Sergey V Kraevskiy Sergei G Ignatov Tatyana E Ignatyuk Igor V Yaminsky Nikolay V Volozhantsev |
| author_sort | Evgeniy V Dubrovin |
| collection | DOAJ |
| description | BACKGROUND: Acinetobacter baumannii is known for its ability to develop resistance to the major groups of antibiotics, form biofilms, and survive for long periods in hospital environments. The prevalence of infections caused by multidrug-resistant A. baumannii is a significant problem for the modern health care system, and application of lytic bacteriophages for controlling this pathogen may become a solution. METHODOLOGY/PRINCIPAL FINDINGS: In this study, using atomic force microscopy (AFM) and microbiological assessment we have investigated A. baumannii bacteriophage AP22, which has been recently described. AFM has revealed the morphology of bacteriophage AP22, adsorbed on the surfaces of mica, graphite and host bacterial cells. Besides, morphological changes of bacteriophage AP22-infected A. baumannii cells were characterized at different stages of the lytic cycle, from phage adsorption to the cell lysis. The phage latent period, estimated from AFM was in good agreement with that obtained by microbiological methods (40 min). Bacteriophage AP22, whose head diameter is 62±1 nm and tail length is 88±9 nm, was shown to disperse A. baumannii aggregates and adsorb to the bacterial surface right from the first minute of their mutual incubation at 37°C. CONCLUSIONS/SIGNIFICANCE: High rate of bacteriophage AP22 specific adsorption and its ability to disperse bacterial aggregates make this phage very promising for biomedical antimicrobial applications. Complementing microbiological results with AFM data, we demonstrate an effective approach, which allows not only comparing independently obtained characteristics of the lytic cycle but also visualizing the infection process. |
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| id | doaj.art-9851ef0d32ce4c42a5b6f206782d7148 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-24T00:12:46Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9851ef0d32ce4c42a5b6f206782d71482022-12-21T17:24:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01710e4734810.1371/journal.pone.0047348Atomic force microscopy analysis of the Acinetobacter baumannii bacteriophage AP22 lytic cycle.Evgeniy V DubrovinAnastasia V PopovaSergey V KraevskiySergei G IgnatovTatyana E IgnatyukIgor V YaminskyNikolay V VolozhantsevBACKGROUND: Acinetobacter baumannii is known for its ability to develop resistance to the major groups of antibiotics, form biofilms, and survive for long periods in hospital environments. The prevalence of infections caused by multidrug-resistant A. baumannii is a significant problem for the modern health care system, and application of lytic bacteriophages for controlling this pathogen may become a solution. METHODOLOGY/PRINCIPAL FINDINGS: In this study, using atomic force microscopy (AFM) and microbiological assessment we have investigated A. baumannii bacteriophage AP22, which has been recently described. AFM has revealed the morphology of bacteriophage AP22, adsorbed on the surfaces of mica, graphite and host bacterial cells. Besides, morphological changes of bacteriophage AP22-infected A. baumannii cells were characterized at different stages of the lytic cycle, from phage adsorption to the cell lysis. The phage latent period, estimated from AFM was in good agreement with that obtained by microbiological methods (40 min). Bacteriophage AP22, whose head diameter is 62±1 nm and tail length is 88±9 nm, was shown to disperse A. baumannii aggregates and adsorb to the bacterial surface right from the first minute of their mutual incubation at 37°C. CONCLUSIONS/SIGNIFICANCE: High rate of bacteriophage AP22 specific adsorption and its ability to disperse bacterial aggregates make this phage very promising for biomedical antimicrobial applications. Complementing microbiological results with AFM data, we demonstrate an effective approach, which allows not only comparing independently obtained characteristics of the lytic cycle but also visualizing the infection process.http://europepmc.org/articles/PMC3469531?pdf=render |
| spellingShingle | Evgeniy V Dubrovin Anastasia V Popova Sergey V Kraevskiy Sergei G Ignatov Tatyana E Ignatyuk Igor V Yaminsky Nikolay V Volozhantsev Atomic force microscopy analysis of the Acinetobacter baumannii bacteriophage AP22 lytic cycle. PLoS ONE |
| title | Atomic force microscopy analysis of the Acinetobacter baumannii bacteriophage AP22 lytic cycle. |
| title_full | Atomic force microscopy analysis of the Acinetobacter baumannii bacteriophage AP22 lytic cycle. |
| title_fullStr | Atomic force microscopy analysis of the Acinetobacter baumannii bacteriophage AP22 lytic cycle. |
| title_full_unstemmed | Atomic force microscopy analysis of the Acinetobacter baumannii bacteriophage AP22 lytic cycle. |
| title_short | Atomic force microscopy analysis of the Acinetobacter baumannii bacteriophage AP22 lytic cycle. |
| title_sort | atomic force microscopy analysis of the acinetobacter baumannii bacteriophage ap22 lytic cycle |
| url | http://europepmc.org/articles/PMC3469531?pdf=render |
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