Isolation and in-vitro characterization of extracellular phytase producing bacterial isolates for potential application in poultry feed
Abstract Background Phytase catalyses the breakdown of complex organic forms of phosphorous into simpler forms by sequential hydrolysis of phosphate ester bonds to liberate the inorganic phosphate. Supplementation of feeds with bacterial phytase therefore could enhance the bioavailability of phospho...
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BMC
2023-10-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-03041-2 |
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| author | Lubaba Amede Mussa Diriba Muleta Yadetie Endeshaw Abatenh Temesgen Anteneh Tesfaye Tefera Mesfin Tafesse Gemeda |
| author_facet | Lubaba Amede Mussa Diriba Muleta Yadetie Endeshaw Abatenh Temesgen Anteneh Tesfaye Tefera Mesfin Tafesse Gemeda |
| author_sort | Lubaba Amede Mussa |
| collection | DOAJ |
| description | Abstract Background Phytase catalyses the breakdown of complex organic forms of phosphorous into simpler forms by sequential hydrolysis of phosphate ester bonds to liberate the inorganic phosphate. Supplementation of feeds with bacterial phytase therefore could enhance the bioavailability of phosphorus and micronutrients. Hence, the aim of this study was to isolate and characterize phytase producing bacteria from rhizosphere soil, fresh poultry excreta, and cattle shed to evaluate their potential in improving poultry feeds. Phytase producing bacteria were isolated using wheat bran extract medium. Results A total of 169 bacterial isolates were purified and screened for phytase activity. Out of these, 36 were confirmed as positive for phytase enzyme activity. The bacterial isolates were identified by cultural, morphological, and biochemical features. The isolates were also identified by using 16 S rRNA gene sequencing. The bacterial isolates (RS1, RS8, RS10 and RS15) were provided with gene bank database accession numbers of MZ407562, MZ407563, MZ407564 and MZ407565 respectively. All isolates increased phytase production when cultured in wheat bran extract medium (pH 6) supplemented with 1% (wt/v) galactose and 1% (wt/v) ammonium sulphate incubated at 50oC for 72 h. Proximate composition analysis after supplementation of phytase showed that phytase supplementation improved bioavailability of phosphorus, calcium, potassium and sodium in poultry feed. Conclusions Overall, this study showed that the nutritional value of poultry feed can be improved using microbial phytase enzyme which reduces the cost of supplementation with inorganic phosphate. |
| first_indexed | 2024-03-10T22:13:32Z |
| format | Article |
| id | doaj.art-9852e07c7e6f45688364357632a8c5d4 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-10T22:13:32Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-9852e07c7e6f45688364357632a8c5d42023-11-19T12:32:22ZengBMCBMC Microbiology1471-21802023-10-012311910.1186/s12866-023-03041-2Isolation and in-vitro characterization of extracellular phytase producing bacterial isolates for potential application in poultry feedLubaba Amede Mussa0Diriba Muleta Yadetie1Endeshaw Abatenh Temesgen2Anteneh Tesfaye Tefera3Mesfin Tafesse Gemeda4Biotechnology Department, College of Natural and Computational Sciences, Institute of Biotechnology, Addis Ababa UniversityBiotechnology Department, College of Natural and Computational Sciences, Institute of Biotechnology, Addis Ababa UniversityBiotechnology and Bioprocess Center of Excellence, Addis Ababa Science and Technology UniversityBiotechnology Department, College of Natural and Computational Sciences, Institute of Biotechnology, Addis Ababa UniversityBiotechnology and Bioprocess Center of Excellence, Addis Ababa Science and Technology UniversityAbstract Background Phytase catalyses the breakdown of complex organic forms of phosphorous into simpler forms by sequential hydrolysis of phosphate ester bonds to liberate the inorganic phosphate. Supplementation of feeds with bacterial phytase therefore could enhance the bioavailability of phosphorus and micronutrients. Hence, the aim of this study was to isolate and characterize phytase producing bacteria from rhizosphere soil, fresh poultry excreta, and cattle shed to evaluate their potential in improving poultry feeds. Phytase producing bacteria were isolated using wheat bran extract medium. Results A total of 169 bacterial isolates were purified and screened for phytase activity. Out of these, 36 were confirmed as positive for phytase enzyme activity. The bacterial isolates were identified by cultural, morphological, and biochemical features. The isolates were also identified by using 16 S rRNA gene sequencing. The bacterial isolates (RS1, RS8, RS10 and RS15) were provided with gene bank database accession numbers of MZ407562, MZ407563, MZ407564 and MZ407565 respectively. All isolates increased phytase production when cultured in wheat bran extract medium (pH 6) supplemented with 1% (wt/v) galactose and 1% (wt/v) ammonium sulphate incubated at 50oC for 72 h. Proximate composition analysis after supplementation of phytase showed that phytase supplementation improved bioavailability of phosphorus, calcium, potassium and sodium in poultry feed. Conclusions Overall, this study showed that the nutritional value of poultry feed can be improved using microbial phytase enzyme which reduces the cost of supplementation with inorganic phosphate.https://doi.org/10.1186/s12866-023-03041-2PhytaseRhizosphere soilPoultry feed |
| spellingShingle | Lubaba Amede Mussa Diriba Muleta Yadetie Endeshaw Abatenh Temesgen Anteneh Tesfaye Tefera Mesfin Tafesse Gemeda Isolation and in-vitro characterization of extracellular phytase producing bacterial isolates for potential application in poultry feed BMC Microbiology Phytase Rhizosphere soil Poultry feed |
| title | Isolation and in-vitro characterization of extracellular phytase producing bacterial isolates for potential application in poultry feed |
| title_full | Isolation and in-vitro characterization of extracellular phytase producing bacterial isolates for potential application in poultry feed |
| title_fullStr | Isolation and in-vitro characterization of extracellular phytase producing bacterial isolates for potential application in poultry feed |
| title_full_unstemmed | Isolation and in-vitro characterization of extracellular phytase producing bacterial isolates for potential application in poultry feed |
| title_short | Isolation and in-vitro characterization of extracellular phytase producing bacterial isolates for potential application in poultry feed |
| title_sort | isolation and in vitro characterization of extracellular phytase producing bacterial isolates for potential application in poultry feed |
| topic | Phytase Rhizosphere soil Poultry feed |
| url | https://doi.org/10.1186/s12866-023-03041-2 |
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