DEVELOPMENT OF OPTIMAL EXPERIMENTAL THERAPEUTIC REGIMENS ADMINISTRATION OF IMMOBILIZED INTERFERON α-2b
The development of optimal experimental therapeutic schemes of immobilized interferon α-2b (production of JSC «Siberian Center for Pharmacology and Biotechnology», Novosibirsk) administration was conducted in the frame of the common program of pre-clinical study. The drug being studied was administe...
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2014-10-01
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| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/230 |
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| author | O. G. Shitikova E. Yu. Sherstoboev N. V. Masnaya M. G. Danilets E. S. Trofimova A. A. Ligacheva P. G. Madonov D. N. Kinsht K. I. Ershov M. A. Shilova |
| author_facet | O. G. Shitikova E. Yu. Sherstoboev N. V. Masnaya M. G. Danilets E. S. Trofimova A. A. Ligacheva P. G. Madonov D. N. Kinsht K. I. Ershov M. A. Shilova |
| author_sort | O. G. Shitikova |
| collection | DOAJ |
| description | The development of optimal experimental therapeutic schemes of immobilized interferon α-2b (production of JSC «Siberian Center for Pharmacology and Biotechnology», Novosibirsk) administration was conducted in the frame of the common program of pre-clinical study. The drug being studied was administered intragastrically for 3, 5 and 7 days in the therapeutic dose (1.8 × 105 IU/kg). Experimental evaluation of immunotropic properties of immobilized interferon α-2b was carried out at in vivoexperiments in outbred CD1 male mice with using of following tests: the study of drug effect on the phagocytic activity of peritoneal leukocytes (the relative number of phagocytes and the number of bacteria which were assimilated by phagocytes were assessed); the study of drug effect on the humoral immune response (the total number of splenocytes, the relative and absolute number of antibody-forming cells and their functional activity were determined); estimation of drug effect on the cellular immune response with the using of delayed-type hypersensitivity test induced by sheep red blood cells. Based on these data we concluded that course administration of immobilized interferon α-2b in the therapeutic dose for 5 days increased phagocytosis of neutrophils and humoral immune response most effectively. Cell-mediated immune response was significantly increased after 7-fold administration of studied drug. |
| first_indexed | 2024-04-10T01:07:43Z |
| format | Article |
| id | doaj.art-98565364c2df4e198decb78182bdacf3 |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:07:43Z |
| publishDate | 2014-10-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-98565364c2df4e198decb78182bdacf32023-03-13T09:58:20ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842014-10-0113511412110.20538/1682-0363-2014-5-114-121228DEVELOPMENT OF OPTIMAL EXPERIMENTAL THERAPEUTIC REGIMENS ADMINISTRATION OF IMMOBILIZED INTERFERON α-2bO. G. Shitikova0E. Yu. Sherstoboev1N. V. Masnaya2M. G. Danilets3E. S. Trofimova4A. A. Ligacheva5P. G. Madonov6D. N. Kinsht7K. I. Ershov8M. A. Shilova9НИИ фармакологии им. Е.Д. Гольдберга, ТомскНИИ фармакологии им. Е.Д. Гольдберга, ТомскНИИ фармакологии им. Е.Д. Гольдберга, ТомскНИИ фармакологии им. Е.Д. Гольдберга, ТомскНИИ фармакологии им. Е.Д. Гольдберга, ТомскНИИ фармакологии им. Е.Д. Гольдберга, ТомскСибирский центр фармакологии и биотехнологии, НовосибирскСибирский центр фармакологии и биотехнологии, НовосибирскСибирский центр фармакологии и биотехнологии, НовосибирскСибирский центр фармакологии и биотехнологии, НовосибирскThe development of optimal experimental therapeutic schemes of immobilized interferon α-2b (production of JSC «Siberian Center for Pharmacology and Biotechnology», Novosibirsk) administration was conducted in the frame of the common program of pre-clinical study. The drug being studied was administered intragastrically for 3, 5 and 7 days in the therapeutic dose (1.8 × 105 IU/kg). Experimental evaluation of immunotropic properties of immobilized interferon α-2b was carried out at in vivoexperiments in outbred CD1 male mice with using of following tests: the study of drug effect on the phagocytic activity of peritoneal leukocytes (the relative number of phagocytes and the number of bacteria which were assimilated by phagocytes were assessed); the study of drug effect on the humoral immune response (the total number of splenocytes, the relative and absolute number of antibody-forming cells and their functional activity were determined); estimation of drug effect on the cellular immune response with the using of delayed-type hypersensitivity test induced by sheep red blood cells. Based on these data we concluded that course administration of immobilized interferon α-2b in the therapeutic dose for 5 days increased phagocytosis of neutrophils and humoral immune response most effectively. Cell-mediated immune response was significantly increased after 7-fold administration of studied drug.https://bulletin.ssmu.ru/jour/article/view/230пегилированиеиммобилизированный интерферон α-2bаутбредные мышисхема введения |
| spellingShingle | O. G. Shitikova E. Yu. Sherstoboev N. V. Masnaya M. G. Danilets E. S. Trofimova A. A. Ligacheva P. G. Madonov D. N. Kinsht K. I. Ershov M. A. Shilova DEVELOPMENT OF OPTIMAL EXPERIMENTAL THERAPEUTIC REGIMENS ADMINISTRATION OF IMMOBILIZED INTERFERON α-2b Бюллетень сибирской медицины пегилирование иммобилизированный интерферон α-2b аутбредные мыши схема введения |
| title | DEVELOPMENT OF OPTIMAL EXPERIMENTAL THERAPEUTIC REGIMENS ADMINISTRATION OF IMMOBILIZED INTERFERON α-2b |
| title_full | DEVELOPMENT OF OPTIMAL EXPERIMENTAL THERAPEUTIC REGIMENS ADMINISTRATION OF IMMOBILIZED INTERFERON α-2b |
| title_fullStr | DEVELOPMENT OF OPTIMAL EXPERIMENTAL THERAPEUTIC REGIMENS ADMINISTRATION OF IMMOBILIZED INTERFERON α-2b |
| title_full_unstemmed | DEVELOPMENT OF OPTIMAL EXPERIMENTAL THERAPEUTIC REGIMENS ADMINISTRATION OF IMMOBILIZED INTERFERON α-2b |
| title_short | DEVELOPMENT OF OPTIMAL EXPERIMENTAL THERAPEUTIC REGIMENS ADMINISTRATION OF IMMOBILIZED INTERFERON α-2b |
| title_sort | development of optimal experimental therapeutic regimens administration of immobilized interferon α 2b |
| topic | пегилирование иммобилизированный интерферон α-2b аутбредные мыши схема введения |
| url | https://bulletin.ssmu.ru/jour/article/view/230 |
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