Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut

Abstract Background Development of oligonucleotide probes facilitates chromosome identification via fluorescence in situ hybridization (FISH) in many organisms. Results We report a high throughput and economical method of chromosome identification based on the development of a dye solution containin...

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Main Authors: Pei Du, Caihong Cui, Hua Liu, Liuyang Fu, Lina Li, Xiaodong Dai, Li Qin, Siyu Wang, Suoyi Han, Jing Xu, Bing Liu, Bingyan Huang, Fengshou Tang, Wenzhao Dong, Zengjun Qi, Xinyou Zhang
Format: Article
Language:English
Published: BMC 2019-07-01
Series:Plant Methods
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13007-019-0451-7
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author Pei Du
Caihong Cui
Hua Liu
Liuyang Fu
Lina Li
Xiaodong Dai
Li Qin
Siyu Wang
Suoyi Han
Jing Xu
Bing Liu
Bingyan Huang
Fengshou Tang
Wenzhao Dong
Zengjun Qi
Xinyou Zhang
author_facet Pei Du
Caihong Cui
Hua Liu
Liuyang Fu
Lina Li
Xiaodong Dai
Li Qin
Siyu Wang
Suoyi Han
Jing Xu
Bing Liu
Bingyan Huang
Fengshou Tang
Wenzhao Dong
Zengjun Qi
Xinyou Zhang
author_sort Pei Du
collection DOAJ
description Abstract Background Development of oligonucleotide probes facilitates chromosome identification via fluorescence in situ hybridization (FISH) in many organisms. Results We report a high throughput and economical method of chromosome identification based on the development of a dye solution containing 2 × saline-sodium citrate (SSC) and oligonucleotide probes. Based on the concentration, staining time, and sequence effects of oligonucleotides, an efficient probe dye of peanut was developed for chromosome identification. To validate the effects of this solution, 200 slides derived from 21 accessions of the cultivated peanut and 30 wild Arachis species were painted to identify Arachis genomes and establish karyotypes. The results showed that one jar of dye could be used to paint 10 chromosome preparations and recycled at least 10 times to efficiently dye more than 100 slides. The A, B, K, F, E, and H genomes showed unique staining karyotype patterns and signal colors. Conclusions Based on the karyotype patterns of Arachis genomes, we revealed the relationships among the A, B, K, F, E, and H genomes in genus Arachis, and demonstrated the potential for adoption of this oligonucleotide dye solution in practice.
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spelling doaj.art-98589ed634624852afca45d3b7257d682022-12-22T01:14:16ZengBMCPlant Methods1746-48112019-07-0115111210.1186/s13007-019-0451-7Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanutPei Du0Caihong Cui1Hua Liu2Liuyang Fu3Lina Li4Xiaodong Dai5Li Qin6Siyu Wang7Suoyi Han8Jing Xu9Bing Liu10Bingyan Huang11Fengshou Tang12Wenzhao Dong13Zengjun Qi14Xinyou Zhang15Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementSchool of Life Sciences, Zhengzhou UniversityIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementSchool of Life Sciences, Zhengzhou UniversityIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementAbstract Background Development of oligonucleotide probes facilitates chromosome identification via fluorescence in situ hybridization (FISH) in many organisms. Results We report a high throughput and economical method of chromosome identification based on the development of a dye solution containing 2 × saline-sodium citrate (SSC) and oligonucleotide probes. Based on the concentration, staining time, and sequence effects of oligonucleotides, an efficient probe dye of peanut was developed for chromosome identification. To validate the effects of this solution, 200 slides derived from 21 accessions of the cultivated peanut and 30 wild Arachis species were painted to identify Arachis genomes and establish karyotypes. The results showed that one jar of dye could be used to paint 10 chromosome preparations and recycled at least 10 times to efficiently dye more than 100 slides. The A, B, K, F, E, and H genomes showed unique staining karyotype patterns and signal colors. Conclusions Based on the karyotype patterns of Arachis genomes, we revealed the relationships among the A, B, K, F, E, and H genomes in genus Arachis, and demonstrated the potential for adoption of this oligonucleotide dye solution in practice.http://link.springer.com/article/10.1186/s13007-019-0451-7Arachis speciesOligonucleotide dye solutionFISHKaryotype
spellingShingle Pei Du
Caihong Cui
Hua Liu
Liuyang Fu
Lina Li
Xiaodong Dai
Li Qin
Siyu Wang
Suoyi Han
Jing Xu
Bing Liu
Bingyan Huang
Fengshou Tang
Wenzhao Dong
Zengjun Qi
Xinyou Zhang
Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut
Plant Methods
Arachis species
Oligonucleotide dye solution
FISH
Karyotype
title Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut
title_full Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut
title_fullStr Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut
title_full_unstemmed Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut
title_short Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut
title_sort development of an oligonucleotide dye solution facilitates high throughput and cost efficient chromosome identification in peanut
topic Arachis species
Oligonucleotide dye solution
FISH
Karyotype
url http://link.springer.com/article/10.1186/s13007-019-0451-7
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