Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut
Abstract Background Development of oligonucleotide probes facilitates chromosome identification via fluorescence in situ hybridization (FISH) in many organisms. Results We report a high throughput and economical method of chromosome identification based on the development of a dye solution containin...
Main Authors: | , , , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
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BMC
2019-07-01
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Series: | Plant Methods |
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Online Access: | http://link.springer.com/article/10.1186/s13007-019-0451-7 |
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author | Pei Du Caihong Cui Hua Liu Liuyang Fu Lina Li Xiaodong Dai Li Qin Siyu Wang Suoyi Han Jing Xu Bing Liu Bingyan Huang Fengshou Tang Wenzhao Dong Zengjun Qi Xinyou Zhang |
author_facet | Pei Du Caihong Cui Hua Liu Liuyang Fu Lina Li Xiaodong Dai Li Qin Siyu Wang Suoyi Han Jing Xu Bing Liu Bingyan Huang Fengshou Tang Wenzhao Dong Zengjun Qi Xinyou Zhang |
author_sort | Pei Du |
collection | DOAJ |
description | Abstract Background Development of oligonucleotide probes facilitates chromosome identification via fluorescence in situ hybridization (FISH) in many organisms. Results We report a high throughput and economical method of chromosome identification based on the development of a dye solution containing 2 × saline-sodium citrate (SSC) and oligonucleotide probes. Based on the concentration, staining time, and sequence effects of oligonucleotides, an efficient probe dye of peanut was developed for chromosome identification. To validate the effects of this solution, 200 slides derived from 21 accessions of the cultivated peanut and 30 wild Arachis species were painted to identify Arachis genomes and establish karyotypes. The results showed that one jar of dye could be used to paint 10 chromosome preparations and recycled at least 10 times to efficiently dye more than 100 slides. The A, B, K, F, E, and H genomes showed unique staining karyotype patterns and signal colors. Conclusions Based on the karyotype patterns of Arachis genomes, we revealed the relationships among the A, B, K, F, E, and H genomes in genus Arachis, and demonstrated the potential for adoption of this oligonucleotide dye solution in practice. |
first_indexed | 2024-12-11T08:40:50Z |
format | Article |
id | doaj.art-98589ed634624852afca45d3b7257d68 |
institution | Directory Open Access Journal |
issn | 1746-4811 |
language | English |
last_indexed | 2024-12-11T08:40:50Z |
publishDate | 2019-07-01 |
publisher | BMC |
record_format | Article |
series | Plant Methods |
spelling | doaj.art-98589ed634624852afca45d3b7257d682022-12-22T01:14:16ZengBMCPlant Methods1746-48112019-07-0115111210.1186/s13007-019-0451-7Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanutPei Du0Caihong Cui1Hua Liu2Liuyang Fu3Lina Li4Xiaodong Dai5Li Qin6Siyu Wang7Suoyi Han8Jing Xu9Bing Liu10Bingyan Huang11Fengshou Tang12Wenzhao Dong13Zengjun Qi14Xinyou Zhang15Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementSchool of Life Sciences, Zhengzhou UniversityIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementSchool of Life Sciences, Zhengzhou UniversityIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityIndustrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture/Henan Provincial Key Laboratory for Oil Crops ImprovementAbstract Background Development of oligonucleotide probes facilitates chromosome identification via fluorescence in situ hybridization (FISH) in many organisms. Results We report a high throughput and economical method of chromosome identification based on the development of a dye solution containing 2 × saline-sodium citrate (SSC) and oligonucleotide probes. Based on the concentration, staining time, and sequence effects of oligonucleotides, an efficient probe dye of peanut was developed for chromosome identification. To validate the effects of this solution, 200 slides derived from 21 accessions of the cultivated peanut and 30 wild Arachis species were painted to identify Arachis genomes and establish karyotypes. The results showed that one jar of dye could be used to paint 10 chromosome preparations and recycled at least 10 times to efficiently dye more than 100 slides. The A, B, K, F, E, and H genomes showed unique staining karyotype patterns and signal colors. Conclusions Based on the karyotype patterns of Arachis genomes, we revealed the relationships among the A, B, K, F, E, and H genomes in genus Arachis, and demonstrated the potential for adoption of this oligonucleotide dye solution in practice.http://link.springer.com/article/10.1186/s13007-019-0451-7Arachis speciesOligonucleotide dye solutionFISHKaryotype |
spellingShingle | Pei Du Caihong Cui Hua Liu Liuyang Fu Lina Li Xiaodong Dai Li Qin Siyu Wang Suoyi Han Jing Xu Bing Liu Bingyan Huang Fengshou Tang Wenzhao Dong Zengjun Qi Xinyou Zhang Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut Plant Methods Arachis species Oligonucleotide dye solution FISH Karyotype |
title | Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut |
title_full | Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut |
title_fullStr | Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut |
title_full_unstemmed | Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut |
title_short | Development of an oligonucleotide dye solution facilitates high throughput and cost-efficient chromosome identification in peanut |
title_sort | development of an oligonucleotide dye solution facilitates high throughput and cost efficient chromosome identification in peanut |
topic | Arachis species Oligonucleotide dye solution FISH Karyotype |
url | http://link.springer.com/article/10.1186/s13007-019-0451-7 |
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