Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model
It is not known how biological changes in the lacrimal (LGs) and meibomian (MGs) glands contribute to dry eye disease (DED) in a time-dependent manner. In this study, we investigated time-sequenced changes in the inflammation, oxidative stress, and senescence of stem cells in both glands of an aging...
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Chang Ho Yoon Jin Suk Ryu Ho Sik Hwang Mee Kum Kim |
| author_facet | Chang Ho Yoon Jin Suk Ryu Ho Sik Hwang Mee Kum Kim |
| author_sort | Chang Ho Yoon |
| collection | DOAJ |
| description | It is not known how biological changes in the lacrimal (LGs) and meibomian (MGs) glands contribute to dry eye disease (DED) in a time-dependent manner. In this study, we investigated time-sequenced changes in the inflammation, oxidative stress, and senescence of stem cells in both glands of an aging-related DED mouse model. Eight-week (8W)-, one-year (1Y)-, and two-year (2Y)-old C57BL/6 male mice were used. MG areas of the upper and lower eyelids were analyzed by transillumination meibography imaging. The number of CD45<sup>+</sup>, 8-OHdG<sup>+</sup>, Ki-67<sup>+</sup>, and BrdU<sup>+</sup> cells was compared in both glands. Increased corneal staining and decreased tear secretion were observed in aged mice. The MG dropout area increased with aging, and the age-adjusted MG area in lower lids was negatively correlated with the National Eye Institute (NEI) score. Increased CD4<sup>+</sup> interferon (IFN)-γ<sup>+</sup> cells in LGs were found in both aged mice. An increase in 8-OHdG<sup>+</sup> cells in both glands was evident in 2Y-old mice. Reduced Ki-67<sup>+</sup> cells, but no change in CD45<sup>+</sup> cells, was observed in the MGs of 1Y-old mice. Increased BrdU<sup>+</sup> cells were observed in the LGs of aged mice. This suggests that age-dependent DED in C57BL/6 mice is related to inflammation of the LGs, the development of MG atrophy, and oxidative stress in both glands. |
| first_indexed | 2024-03-09T06:15:50Z |
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| language | English |
| last_indexed | 2024-03-09T06:15:50Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9858e3b3e2aa44fc806e4d318adf26cc2023-12-03T11:53:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012111416910.3390/ijms21114169Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse ModelChang Ho Yoon0Jin Suk Ryu1Ho Sik Hwang2Mee Kum Kim3Department of Ophthalmology, Seoul National University College of Medicine, Seoul 03080, KoreaLaboratory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Biomedical Research Institute, Seoul 03080, KoreaDepartment of Ophthalmology, College of Medicine, The Catholic University of Korea, Seoul 07345, KoreaDepartment of Ophthalmology, Seoul National University College of Medicine, Seoul 03080, KoreaIt is not known how biological changes in the lacrimal (LGs) and meibomian (MGs) glands contribute to dry eye disease (DED) in a time-dependent manner. In this study, we investigated time-sequenced changes in the inflammation, oxidative stress, and senescence of stem cells in both glands of an aging-related DED mouse model. Eight-week (8W)-, one-year (1Y)-, and two-year (2Y)-old C57BL/6 male mice were used. MG areas of the upper and lower eyelids were analyzed by transillumination meibography imaging. The number of CD45<sup>+</sup>, 8-OHdG<sup>+</sup>, Ki-67<sup>+</sup>, and BrdU<sup>+</sup> cells was compared in both glands. Increased corneal staining and decreased tear secretion were observed in aged mice. The MG dropout area increased with aging, and the age-adjusted MG area in lower lids was negatively correlated with the National Eye Institute (NEI) score. Increased CD4<sup>+</sup> interferon (IFN)-γ<sup>+</sup> cells in LGs were found in both aged mice. An increase in 8-OHdG<sup>+</sup> cells in both glands was evident in 2Y-old mice. Reduced Ki-67<sup>+</sup> cells, but no change in CD45<sup>+</sup> cells, was observed in the MGs of 1Y-old mice. Increased BrdU<sup>+</sup> cells were observed in the LGs of aged mice. This suggests that age-dependent DED in C57BL/6 mice is related to inflammation of the LGs, the development of MG atrophy, and oxidative stress in both glands.https://www.mdpi.com/1422-0067/21/11/4169dry eyeaginglacrimal glandsmeibomian glandsinflammationoxidative stress |
| spellingShingle | Chang Ho Yoon Jin Suk Ryu Ho Sik Hwang Mee Kum Kim Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model International Journal of Molecular Sciences dry eye aging lacrimal glands meibomian glands inflammation oxidative stress |
| title | Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model |
| title_full | Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model |
| title_fullStr | Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model |
| title_full_unstemmed | Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model |
| title_short | Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model |
| title_sort | comparative analysis of age related changes in lacrimal glands and meibomian glands of a c57bl 6 male mouse model |
| topic | dry eye aging lacrimal glands meibomian glands inflammation oxidative stress |
| url | https://www.mdpi.com/1422-0067/21/11/4169 |
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