Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii
The polyamine putrescine (Put) is a ubiquitous small cationic amine. It plays an essential role in controlling the innate immune response. However, little is known about its function in mollusks. In this study, the Put content was observed to increase in the serum of pearl oyster Pinctada fucata mar...
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.781324/full |
| _version_ | 1818388176130015232 |
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| author | Yanfei Cao Yu Jiao Yu Jiao Yu Jiao Yu Jiao Shuzhi Zhan Xueru Liang Zhixin Li Jiayi Chen Xinwei Xiong Zefeng Gu Xiaodong Du Xiaodong Du Xiaodong Du Xiaodong Du Zhe Zheng Zhe Zheng Zhe Zheng Zhe Zheng |
| author_facet | Yanfei Cao Yu Jiao Yu Jiao Yu Jiao Yu Jiao Shuzhi Zhan Xueru Liang Zhixin Li Jiayi Chen Xinwei Xiong Zefeng Gu Xiaodong Du Xiaodong Du Xiaodong Du Xiaodong Du Zhe Zheng Zhe Zheng Zhe Zheng Zhe Zheng |
| author_sort | Yanfei Cao |
| collection | DOAJ |
| description | The polyamine putrescine (Put) is a ubiquitous small cationic amine. It plays an essential role in controlling the innate immune response. However, little is known about its function in mollusks. In this study, the Put content was observed to increase in the serum of pearl oyster Pinctada fucata martensii after 6 and 24 h of lipopolysaccharide (LPS) stimulation. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) increased, and nitric oxide synthase was downregulated in the Put group (i.e., combined treatment with Put and LPS) compared with that in the LPS group (i.e., combined treatment with phosphate-buffered saline and LPS). Furthermore, activities of alkaline phosphatase and acid phosphatase were inhibited after 6 h of LPS stimulation. The expression levels of the nuclear factor kappa B, IκB kinase, Janus kinase, and signal transducer and activator of transcription proteins genes were all significantly suppressed at 12 and 24 h in the Put group. Pseudomonas aeruginosa and Bacillus subtilis grew better after being incubated with the serum from the Put group than that from the LPS group. Additionally, the Put treatment remarkably inhibited the autophagy of hemocytes mediated by the AMP-activated protein kinase-mammalian target of rapamycin-Beclin-1 pathway. This study demonstrated that Put can effectively inhibit the inflammatory response induced by LPS in pearl oysters. These results provide useful information for further exploration of the immunoregulatory functions of polyamines in bivalves and contribute to the development of immunosuppressive agents. |
| first_indexed | 2024-12-14T04:21:40Z |
| format | Article |
| id | doaj.art-9859e02d83b94d7cbc8061037fc82264 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-14T04:21:40Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-9859e02d83b94d7cbc8061037fc822642022-12-21T23:17:19ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-12-011210.3389/fphys.2021.781324781324Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensiiYanfei Cao0Yu Jiao1Yu Jiao2Yu Jiao3Yu Jiao4Shuzhi Zhan5Xueru Liang6Zhixin Li7Jiayi Chen8Xinwei Xiong9Zefeng Gu10Xiaodong Du11Xiaodong Du12Xiaodong Du13Xiaodong Du14Zhe Zheng15Zhe Zheng16Zhe Zheng17Zhe Zheng18Fishery College, Guangdong Ocean University, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaPearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, ChinaGuangdong Science and Innovation Center for Pearl Culture, Zhanjiang, ChinaGuangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaPearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, ChinaGuangdong Science and Innovation Center for Pearl Culture, Zhanjiang, ChinaGuangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang, ChinaFishery College, Guangdong Ocean University, Zhanjiang, ChinaPearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, ChinaGuangdong Science and Innovation Center for Pearl Culture, Zhanjiang, ChinaGuangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang, ChinaThe polyamine putrescine (Put) is a ubiquitous small cationic amine. It plays an essential role in controlling the innate immune response. However, little is known about its function in mollusks. In this study, the Put content was observed to increase in the serum of pearl oyster Pinctada fucata martensii after 6 and 24 h of lipopolysaccharide (LPS) stimulation. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) increased, and nitric oxide synthase was downregulated in the Put group (i.e., combined treatment with Put and LPS) compared with that in the LPS group (i.e., combined treatment with phosphate-buffered saline and LPS). Furthermore, activities of alkaline phosphatase and acid phosphatase were inhibited after 6 h of LPS stimulation. The expression levels of the nuclear factor kappa B, IκB kinase, Janus kinase, and signal transducer and activator of transcription proteins genes were all significantly suppressed at 12 and 24 h in the Put group. Pseudomonas aeruginosa and Bacillus subtilis grew better after being incubated with the serum from the Put group than that from the LPS group. Additionally, the Put treatment remarkably inhibited the autophagy of hemocytes mediated by the AMP-activated protein kinase-mammalian target of rapamycin-Beclin-1 pathway. This study demonstrated that Put can effectively inhibit the inflammatory response induced by LPS in pearl oysters. These results provide useful information for further exploration of the immunoregulatory functions of polyamines in bivalves and contribute to the development of immunosuppressive agents.https://www.frontiersin.org/articles/10.3389/fphys.2021.781324/fullpolyaminesputrescineimmune regulationoxidative stressmolluskspearl oysters |
| spellingShingle | Yanfei Cao Yu Jiao Yu Jiao Yu Jiao Yu Jiao Shuzhi Zhan Xueru Liang Zhixin Li Jiayi Chen Xinwei Xiong Zefeng Gu Xiaodong Du Xiaodong Du Xiaodong Du Xiaodong Du Zhe Zheng Zhe Zheng Zhe Zheng Zhe Zheng Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii Frontiers in Physiology polyamines putrescine immune regulation oxidative stress mollusks pearl oysters |
| title | Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii |
| title_full | Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii |
| title_fullStr | Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii |
| title_full_unstemmed | Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii |
| title_short | Polyamine Putrescine Regulates Oxidative Stress and Autophagy of Hemocytes Induced by Lipopolysaccharides in Pearl Oyster Pinctada fucata martensii |
| title_sort | polyamine putrescine regulates oxidative stress and autophagy of hemocytes induced by lipopolysaccharides in pearl oyster pinctada fucata martensii |
| topic | polyamines putrescine immune regulation oxidative stress mollusks pearl oysters |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2021.781324/full |
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