Transcriptomic profile of epileptic children treated with ketogenic therapies
Background: Ketogenic dietary therapies (KDT) are used as a treatment in childhood epilepsy. However, their mechanism has not yet been established. The main objective of this study was to determine the changes in the transcriptomic profile induced by KDT in children with epilepsy in order to shed...
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| Format: | Article |
| Language: | English |
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IMR Press
2022-01-01
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| Series: | Journal of Integrative Neuroscience |
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| Online Access: | https://article.imrpress.com/journal/JIN/21/1/10.31083/j.jin2101031/1757-448X-21-1-031.pdf |
| _version_ | 1811291395266183168 |
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| author | Jana Ruiz-Herrero Gloria Olaso-Gonzalez Eva Serna Elvira Cañedo-Villarroya Angela G. Correas Juan Gambini Mari Carmen Gomez-Cabrera Consuelo Pedrón-Giner Jose Vina |
| author_facet | Jana Ruiz-Herrero Gloria Olaso-Gonzalez Eva Serna Elvira Cañedo-Villarroya Angela G. Correas Juan Gambini Mari Carmen Gomez-Cabrera Consuelo Pedrón-Giner Jose Vina |
| author_sort | Jana Ruiz-Herrero |
| collection | DOAJ |
| description | Background: Ketogenic dietary therapies (KDT) are used as a treatment
in childhood epilepsy. However, their mechanism has not yet been established. The
main objective of this study was to determine the changes in the transcriptomic
profile induced by KDT in children with epilepsy in order to shed light on its
possible mechanisms. Methods: Eight children with refractory epilepsy
were enrolled in the study. Peripheral blood mononuclear cells were obtained
before and after the children were treated with KDT for a minimum of 6 months.
RNA was extracted and mRNA and miRNA profiling were performed and analyzed.
Results: Our intervention with KDT significantly reduced the seizure
number in seven of the eight paediatric patients treated and caused important
changes in their gene expression profile. Our study reveals modifications in the
transcription of 4630 genes and 230 miRNAs. We found that the genes involved in
the protection against epileptic crises were among those mainly changed. These
genes collectively encode for ion channels, neurotransmitter receptors, and
synapse structural proteins. Conclusions: Together our results explain
the possible mechanisms of KDT and reinforce its clinical importance in the
treatment of epilepsy. |
| first_indexed | 2024-04-13T04:28:12Z |
| format | Article |
| id | doaj.art-985b98d3bec042bc816164a1321ed42c |
| institution | Directory Open Access Journal |
| issn | 0219-6352 |
| language | English |
| last_indexed | 2024-04-13T04:28:12Z |
| publishDate | 2022-01-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Journal of Integrative Neuroscience |
| spelling | doaj.art-985b98d3bec042bc816164a1321ed42c2022-12-22T03:02:25ZengIMR PressJournal of Integrative Neuroscience0219-63522022-01-0121103110.31083/j.jin2101031S0219-6352(22)00291-1Transcriptomic profile of epileptic children treated with ketogenic therapiesJana Ruiz-Herrero0Gloria Olaso-Gonzalez1Eva Serna2Elvira Cañedo-Villarroya3Angela G. Correas4Juan Gambini5Mari Carmen Gomez-Cabrera6Consuelo Pedrón-Giner7Jose Vina8Department of Pediatric Gastroenterology, Pediatric Service, San Rafael Hospital, 2016 Madrid, SpainFreshage Research Group, Department of Physiology, Faculty of Medicine, University of Valencia, CIBERFES, Fundación Investigación Hospital Clínico Universitario/INCLIVA, 46010 Valencia, SpainFreshage Research Group, Department of Physiology, Faculty of Medicine, University of Valencia, CIBERFES, Fundación Investigación Hospital Clínico Universitario/INCLIVA, 46010 Valencia, SpainDepartment of Gastroenterology and Nutrition, Niño Jesús Pediatric Hospital, 28009 Madrid, SpainFreshage Research Group, Department of Physiology, Faculty of Medicine, University of Valencia, CIBERFES, Fundación Investigación Hospital Clínico Universitario/INCLIVA, 46010 Valencia, SpainFreshage Research Group, Department of Physiology, Faculty of Medicine, University of Valencia, CIBERFES, Fundación Investigación Hospital Clínico Universitario/INCLIVA, 46010 Valencia, SpainFreshage Research Group, Department of Physiology, Faculty of Medicine, University of Valencia, CIBERFES, Fundación Investigación Hospital Clínico Universitario/INCLIVA, 46010 Valencia, SpainDepartment of Gastroenterology and Nutrition, Niño Jesús Pediatric Hospital, 28009 Madrid, SpainFreshage Research Group, Department of Physiology, Faculty of Medicine, University of Valencia, CIBERFES, Fundación Investigación Hospital Clínico Universitario/INCLIVA, 46010 Valencia, SpainBackground: Ketogenic dietary therapies (KDT) are used as a treatment in childhood epilepsy. However, their mechanism has not yet been established. The main objective of this study was to determine the changes in the transcriptomic profile induced by KDT in children with epilepsy in order to shed light on its possible mechanisms. Methods: Eight children with refractory epilepsy were enrolled in the study. Peripheral blood mononuclear cells were obtained before and after the children were treated with KDT for a minimum of 6 months. RNA was extracted and mRNA and miRNA profiling were performed and analyzed. Results: Our intervention with KDT significantly reduced the seizure number in seven of the eight paediatric patients treated and caused important changes in their gene expression profile. Our study reveals modifications in the transcription of 4630 genes and 230 miRNAs. We found that the genes involved in the protection against epileptic crises were among those mainly changed. These genes collectively encode for ion channels, neurotransmitter receptors, and synapse structural proteins. Conclusions: Together our results explain the possible mechanisms of KDT and reinforce its clinical importance in the treatment of epilepsy.https://article.imrpress.com/journal/JIN/21/1/10.31083/j.jin2101031/1757-448X-21-1-031.pdfepilepsyketogenic dietketogenic dietary therapiestranscriptomeanticonvulsantsynapsismirnome |
| spellingShingle | Jana Ruiz-Herrero Gloria Olaso-Gonzalez Eva Serna Elvira Cañedo-Villarroya Angela G. Correas Juan Gambini Mari Carmen Gomez-Cabrera Consuelo Pedrón-Giner Jose Vina Transcriptomic profile of epileptic children treated with ketogenic therapies Journal of Integrative Neuroscience epilepsy ketogenic diet ketogenic dietary therapies transcriptome anticonvulsant synapsis mirnome |
| title | Transcriptomic profile of epileptic children treated with ketogenic therapies |
| title_full | Transcriptomic profile of epileptic children treated with ketogenic therapies |
| title_fullStr | Transcriptomic profile of epileptic children treated with ketogenic therapies |
| title_full_unstemmed | Transcriptomic profile of epileptic children treated with ketogenic therapies |
| title_short | Transcriptomic profile of epileptic children treated with ketogenic therapies |
| title_sort | transcriptomic profile of epileptic children treated with ketogenic therapies |
| topic | epilepsy ketogenic diet ketogenic dietary therapies transcriptome anticonvulsant synapsis mirnome |
| url | https://article.imrpress.com/journal/JIN/21/1/10.31083/j.jin2101031/1757-448X-21-1-031.pdf |
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