In-Depth Temporal Transcriptome Profiling of an Alphaherpesvirus Using Nanopore Sequencing

In-Depth Temporal Transcriptome Profiling of an Alphaherpesvirus Using Nanopore Sequencing

In this work, a long-read sequencing (LRS) technique based on the Oxford Nanopore Technology MinION platform was used for quantifying and kinetic characterization of the poly(A) fraction of bovine alphaherpesvirus type 1 (BoHV-1) lytic transcriptome across a 12-h infection period. Amplification-base...

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Bibliographic Details
Main Authors: Dóra Tombácz, Balázs Kakuk, Gábor Torma, Zsolt Csabai, Gábor Gulyás, Vivien Tamás, Zoltán Zádori, Victoria A. Jefferson, Florencia Meyer, Zsolt Boldogkői
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:Viruses
Subjects:
herpesviruses
bovine alphaherpesvirus type 1
transcriptome
transcript isoforms
long-read sequencing
nanopore sequencing
Online Access:https://www.mdpi.com/1999-4915/14/6/1289

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