Asymmetry of Sagittal Otolith Shape Based on Inner Ear Side Tested on Mediterranean Red Mullet (<i>Mullus barbatus</i> Linnaeus, 1758): Comparative Analysis of 2D and 3D Otolith Shape Data
Sagittal otolith shape analysis is one of the most widespread techniques worldwide to discriminate fish stock units, as this proxy integrates both environmental and genetic factors. All previous otolith shape studies have been carried out using two-dimensional (2D) images, a partial representation o...
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MDPI AG
2023-05-01
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| Series: | Symmetry |
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| Online Access: | https://www.mdpi.com/2073-8994/15/5/1067 |
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| author | Nicolas Andrialovanirina Émilie Poisson Caillault Sébastien Couette Rémi Laffont Lauriane Poloni Camille Lutet-Toti Kélig Mahé |
| author_facet | Nicolas Andrialovanirina Émilie Poisson Caillault Sébastien Couette Rémi Laffont Lauriane Poloni Camille Lutet-Toti Kélig Mahé |
| author_sort | Nicolas Andrialovanirina |
| collection | DOAJ |
| description | Sagittal otolith shape analysis is one of the most widespread techniques worldwide to discriminate fish stock units, as this proxy integrates both environmental and genetic factors. All previous otolith shape studies have been carried out using two-dimensional (2D) images, a partial representation of the whole shape of the otolith. However, prior to the identification of stock unit boundaries, the influence of other potential drivers controlling the otolith shape must be analysed to limit their bias. In this study, the presence of asymmetry in otolith shape depending on the inner ear side (i.e., left versus right inner ears) was tested by comparing the approaches of 2D and three-dimensional (3D) sagittal otolith shape analyses. Eighty-two red mullet adults (<i>Mullus barbatus</i>) from three locations in the eastern part of the Mediterranean Sea were studied. Fourier harmonic descriptors computed from 2D outlines and spherical harmonic descriptors computed from 3D meshes were used to evaluate otolith shape variation. The results of a multivariate mixed-effects model from 2D images showed that there was no asymmetry effect of inner ear side on the otolith shape in any location. There was, however, a significant geographical effect for the 2D otolith shape between the Adriatic Sea and the Levantine Sea. In contrast, 3D information showed that both side effects and geographical differences were significant. This is the first study comparing 2D and 3D data showing different results on the same sample of red mullet. These results demonstrate the importance of 3D otolith shape analysis for stock discrimination. |
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| last_indexed | 2024-03-11T03:16:33Z |
| publishDate | 2023-05-01 |
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| series | Symmetry |
| spelling | doaj.art-985ddd7ef4fd47fa9074c1f9a9e55efa2023-11-18T03:30:28ZengMDPI AGSymmetry2073-89942023-05-01155106710.3390/sym15051067Asymmetry of Sagittal Otolith Shape Based on Inner Ear Side Tested on Mediterranean Red Mullet (<i>Mullus barbatus</i> Linnaeus, 1758): Comparative Analysis of 2D and 3D Otolith Shape DataNicolas Andrialovanirina0Émilie Poisson Caillault1Sébastien Couette2Rémi Laffont3Lauriane Poloni4Camille Lutet-Toti5Kélig Mahé6UR 4491, LISIC, Laboratoire d’Informatique Signal et Image de la Côte d’Opale, University of the Littoral Côte d’Opale, 62100 Calais, FranceUR 4491, LISIC, Laboratoire d’Informatique Signal et Image de la Côte d’Opale, University of the Littoral Côte d’Opale, 62100 Calais, FranceEcole Pratique des Hautes Etudes, PSL Université, 75014 Paris, FranceUMR CNRS 6282 Biogéosciences, Université de Bourgogne, 6 Bld Gabriel, 21000 Dijon, FranceEcole Pratique des Hautes Etudes, PSL Université, 75014 Paris, FranceUMR CNRS 6282 Biogéosciences, Université de Bourgogne, 6 Bld Gabriel, 21000 Dijon, FranceIFREMER, Fisheries Laboratory, 150 quai Gambetta, 62321 Boulogne-sur-Mer, FranceSagittal otolith shape analysis is one of the most widespread techniques worldwide to discriminate fish stock units, as this proxy integrates both environmental and genetic factors. All previous otolith shape studies have been carried out using two-dimensional (2D) images, a partial representation of the whole shape of the otolith. However, prior to the identification of stock unit boundaries, the influence of other potential drivers controlling the otolith shape must be analysed to limit their bias. In this study, the presence of asymmetry in otolith shape depending on the inner ear side (i.e., left versus right inner ears) was tested by comparing the approaches of 2D and three-dimensional (3D) sagittal otolith shape analyses. Eighty-two red mullet adults (<i>Mullus barbatus</i>) from three locations in the eastern part of the Mediterranean Sea were studied. Fourier harmonic descriptors computed from 2D outlines and spherical harmonic descriptors computed from 3D meshes were used to evaluate otolith shape variation. The results of a multivariate mixed-effects model from 2D images showed that there was no asymmetry effect of inner ear side on the otolith shape in any location. There was, however, a significant geographical effect for the 2D otolith shape between the Adriatic Sea and the Levantine Sea. In contrast, 3D information showed that both side effects and geographical differences were significant. This is the first study comparing 2D and 3D data showing different results on the same sample of red mullet. These results demonstrate the importance of 3D otolith shape analysis for stock discrimination.https://www.mdpi.com/2073-8994/15/5/1067otolith shapeFourier analysis2D3Deffect of inner ear side |
| spellingShingle | Nicolas Andrialovanirina Émilie Poisson Caillault Sébastien Couette Rémi Laffont Lauriane Poloni Camille Lutet-Toti Kélig Mahé Asymmetry of Sagittal Otolith Shape Based on Inner Ear Side Tested on Mediterranean Red Mullet (<i>Mullus barbatus</i> Linnaeus, 1758): Comparative Analysis of 2D and 3D Otolith Shape Data Symmetry otolith shape Fourier analysis 2D 3D effect of inner ear side |
| title | Asymmetry of Sagittal Otolith Shape Based on Inner Ear Side Tested on Mediterranean Red Mullet (<i>Mullus barbatus</i> Linnaeus, 1758): Comparative Analysis of 2D and 3D Otolith Shape Data |
| title_full | Asymmetry of Sagittal Otolith Shape Based on Inner Ear Side Tested on Mediterranean Red Mullet (<i>Mullus barbatus</i> Linnaeus, 1758): Comparative Analysis of 2D and 3D Otolith Shape Data |
| title_fullStr | Asymmetry of Sagittal Otolith Shape Based on Inner Ear Side Tested on Mediterranean Red Mullet (<i>Mullus barbatus</i> Linnaeus, 1758): Comparative Analysis of 2D and 3D Otolith Shape Data |
| title_full_unstemmed | Asymmetry of Sagittal Otolith Shape Based on Inner Ear Side Tested on Mediterranean Red Mullet (<i>Mullus barbatus</i> Linnaeus, 1758): Comparative Analysis of 2D and 3D Otolith Shape Data |
| title_short | Asymmetry of Sagittal Otolith Shape Based on Inner Ear Side Tested on Mediterranean Red Mullet (<i>Mullus barbatus</i> Linnaeus, 1758): Comparative Analysis of 2D and 3D Otolith Shape Data |
| title_sort | asymmetry of sagittal otolith shape based on inner ear side tested on mediterranean red mullet i mullus barbatus i linnaeus 1758 comparative analysis of 2d and 3d otolith shape data |
| topic | otolith shape Fourier analysis 2D 3D effect of inner ear side |
| url | https://www.mdpi.com/2073-8994/15/5/1067 |
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