Using environmental DNA analyses to assess the occurrence and abundance of the endangered amphidromous fish Plecoglossus altivelis ryukyuensis
The Ryukyu ayu Plecoglossus altivelis ryukyuensis is an endangered amphidromous fish that inhabits rivers in the Ryukyu Archipelago (Japan). Populations of the species have declined dramatically. Consequently, the Ryukyu ayu has been registered as a natural monument in Japan and monitoring surveys w...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2020-01-01
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| Series: | Biodiversity Data Journal |
| Subjects: | |
| Online Access: | https://bdj.pensoft.net/article/39679/download/pdf/ |
| _version_ | 1818754730428465152 |
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| author | Yoshihisa Akamatsu Gen Kume Masuji Gotou Takanori Kono Takuma Fujii Ryutei Inui Yoshihisa Kurita |
| author_facet | Yoshihisa Akamatsu Gen Kume Masuji Gotou Takanori Kono Takuma Fujii Ryutei Inui Yoshihisa Kurita |
| author_sort | Yoshihisa Akamatsu |
| collection | DOAJ |
| description | The Ryukyu ayu Plecoglossus altivelis ryukyuensis is an endangered amphidromous fish that inhabits rivers in the Ryukyu Archipelago (Japan). Populations of the species have declined dramatically. Consequently, the Ryukyu ayu has been registered as a natural monument in Japan and monitoring surveys with direct catching are restricted legally. This restriction, unfortunately, makes monitoring of population abundances difficult and creates a barrier to both advancing understanding of the species’ status and the development of appropriate conservation plans.We developed a non-invasive monitoring methodology using eDNA analyses. We designed a specific quantitative PCR assay for the Ryukyu ayu using the mitochondrial ND4 region. Using this primer/probe set, we conducted eDNA analyses in three rivers on Amami-Ohshima Island. The DNA fragments were amplified from the eDNA extracted from natural water in each river. The numbers of DNA fragments detected were positively correlated with individual counts of fish obtained by visual snorkelling surveys. Our method does not contravene restrictions and facilitates abundance monitoring of this endangered fish species. |
| first_indexed | 2024-12-18T05:27:54Z |
| format | Article |
| id | doaj.art-985f6b715dc441e19269b512d6338c62 |
| institution | Directory Open Access Journal |
| issn | 1314-2836 1314-2828 |
| language | English |
| last_indexed | 2024-12-18T05:27:54Z |
| publishDate | 2020-01-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Biodiversity Data Journal |
| spelling | doaj.art-985f6b715dc441e19269b512d6338c622022-12-21T21:19:31ZengPensoft PublishersBiodiversity Data Journal1314-28361314-28282020-01-01811110.3897/BDJ.8.e3967939679Using environmental DNA analyses to assess the occurrence and abundance of the endangered amphidromous fish Plecoglossus altivelis ryukyuensisYoshihisa Akamatsu0Gen Kume1Masuji Gotou2Takanori Kono3Takuma Fujii4Ryutei Inui5Yoshihisa Kurita6Yamaguchi UniversityKagoshima UniversityYamaguchi UniversityYamaguchi UniversityKagoshima UniversityFukuoka Institute of TechnologyKyushu UniversityThe Ryukyu ayu Plecoglossus altivelis ryukyuensis is an endangered amphidromous fish that inhabits rivers in the Ryukyu Archipelago (Japan). Populations of the species have declined dramatically. Consequently, the Ryukyu ayu has been registered as a natural monument in Japan and monitoring surveys with direct catching are restricted legally. This restriction, unfortunately, makes monitoring of population abundances difficult and creates a barrier to both advancing understanding of the species’ status and the development of appropriate conservation plans.We developed a non-invasive monitoring methodology using eDNA analyses. We designed a specific quantitative PCR assay for the Ryukyu ayu using the mitochondrial ND4 region. Using this primer/probe set, we conducted eDNA analyses in three rivers on Amami-Ohshima Island. The DNA fragments were amplified from the eDNA extracted from natural water in each river. The numbers of DNA fragments detected were positively correlated with individual counts of fish obtained by visual snorkelling surveys. Our method does not contravene restrictions and facilitates abundance monitoring of this endangered fish species.https://bdj.pensoft.net/article/39679/download/pdf/Monitoring methodsthreatened speciesstock mana |
| spellingShingle | Yoshihisa Akamatsu Gen Kume Masuji Gotou Takanori Kono Takuma Fujii Ryutei Inui Yoshihisa Kurita Using environmental DNA analyses to assess the occurrence and abundance of the endangered amphidromous fish Plecoglossus altivelis ryukyuensis Biodiversity Data Journal Monitoring methods threatened species stock mana |
| title | Using environmental DNA analyses to assess the occurrence and abundance of the endangered amphidromous fish Plecoglossus altivelis ryukyuensis |
| title_full | Using environmental DNA analyses to assess the occurrence and abundance of the endangered amphidromous fish Plecoglossus altivelis ryukyuensis |
| title_fullStr | Using environmental DNA analyses to assess the occurrence and abundance of the endangered amphidromous fish Plecoglossus altivelis ryukyuensis |
| title_full_unstemmed | Using environmental DNA analyses to assess the occurrence and abundance of the endangered amphidromous fish Plecoglossus altivelis ryukyuensis |
| title_short | Using environmental DNA analyses to assess the occurrence and abundance of the endangered amphidromous fish Plecoglossus altivelis ryukyuensis |
| title_sort | using environmental dna analyses to assess the occurrence and abundance of the endangered amphidromous fish plecoglossus altivelis ryukyuensis |
| topic | Monitoring methods threatened species stock mana |
| url | https://bdj.pensoft.net/article/39679/download/pdf/ |
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