pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces
Adipose-derived stem cells (ASCs) have raised significant interest for their potential therapeutic applications in regenerative medicine. However, ASCs usually suffer from decreased pluripotency and functional plasticity during in vitro expansion. Herein, this study sought to develop a continuous ce...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S259000642200312X |
| _version_ | 1828048271793192960 |
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| author | Chia-Hsiang Yen Nai-Chen Cheng Hao-Ying Hsieh Ching-Wen Tsai An-Li Lee Chien-Yi Lu Yin-Tzu Chen Tai-Horng Young |
| author_facet | Chia-Hsiang Yen Nai-Chen Cheng Hao-Ying Hsieh Ching-Wen Tsai An-Li Lee Chien-Yi Lu Yin-Tzu Chen Tai-Horng Young |
| author_sort | Chia-Hsiang Yen |
| collection | DOAJ |
| description | Adipose-derived stem cells (ASCs) have raised significant interest for their potential therapeutic applications in regenerative medicine. However, ASCs usually suffer from decreased pluripotency and functional plasticity during in vitro expansion. Herein, this study sought to develop a continuous cell production system that can mass-produce ASCs with sustained regenerative capacity. The strategy was blending pH-responsive chitosan (CS) with polyamide-66 (PA) to generate combined surface properties with controllable cell growth/detachment ability to achieve a repeated cell production process. From the collected data, all the polymer blends were capable of completing a minimum of four consecutive production cycles, wherein the PA17CS blend (PA:CS = 1:7) outperformed with respect to the working effectiveness (average cell detachment ratio= 88%) and the cell viability. Compared to the trypsin-based method, ASCs harvested from PA17CS exhibited superior stemness characteristics along with SDF-1-mediated CXCR4 chemotactic response for stem cell homing. Moreover, injection of ASCs generated from PA17CS blend could more effectively induce neovascularization and protect skin flaps during an ischemic injury in a rat model. |
| first_indexed | 2024-04-10T18:54:53Z |
| format | Article |
| id | doaj.art-9861acae02a2460bad99d106d46a1852 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-10T18:54:53Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-9861acae02a2460bad99d106d46a18522023-02-01T04:27:18ZengElsevierMaterials Today Bio2590-00642023-02-0118100514pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfacesChia-Hsiang Yen0Nai-Chen Cheng1Hao-Ying Hsieh2Ching-Wen Tsai3An-Li Lee4Chien-Yi Lu5Yin-Tzu Chen6Tai-Horng Young7Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, TaiwanDepartment of Surgery, National Taiwan University Hospital and College of Medicine, No. 7, Chung-Shan S Rd., Taipei, 100, TaiwanDepartment of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan; Department of Dentistry, National Taiwan University Hospital, No. 7, Chung-Shan S Rd., Taipei, 100, TaiwanTaiwan Instrument Research Institute, National Applied Research Laboratories, No. 20, R&D Rd. VI, Hsinchu Science Park, Hsinchu, 300, TaiwanDepartment of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan; Department of Plastic and Reconstructive Surgery, MacKay Memorial Hospital, No. 92, Sec. 2, Chung-Shan N Rd., Taipei, 104, Taiwan; Department of Medicine, MacKay Medical College, No. 46, Sec. 3, Zhong-Zheng Rd., New Taipei City, 252, TaiwanDepartment of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, TaiwanDepartment of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, TaiwanDepartment of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan; Department of Biomedical Engineering, National Taiwan University Hospital, No. 7, Chung-Shan S Rd., Taipei, 100, Taiwan; Corresponding author. Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Rd., Taipei, 100, Taiwan.Adipose-derived stem cells (ASCs) have raised significant interest for their potential therapeutic applications in regenerative medicine. However, ASCs usually suffer from decreased pluripotency and functional plasticity during in vitro expansion. Herein, this study sought to develop a continuous cell production system that can mass-produce ASCs with sustained regenerative capacity. The strategy was blending pH-responsive chitosan (CS) with polyamide-66 (PA) to generate combined surface properties with controllable cell growth/detachment ability to achieve a repeated cell production process. From the collected data, all the polymer blends were capable of completing a minimum of four consecutive production cycles, wherein the PA17CS blend (PA:CS = 1:7) outperformed with respect to the working effectiveness (average cell detachment ratio= 88%) and the cell viability. Compared to the trypsin-based method, ASCs harvested from PA17CS exhibited superior stemness characteristics along with SDF-1-mediated CXCR4 chemotactic response for stem cell homing. Moreover, injection of ASCs generated from PA17CS blend could more effectively induce neovascularization and protect skin flaps during an ischemic injury in a rat model.http://www.sciencedirect.com/science/article/pii/S259000642200312XpH-responsive surfacePolyamide-66/chitosanContinuous cell productionSDF-1/CXCR4 axisCell therapy |
| spellingShingle | Chia-Hsiang Yen Nai-Chen Cheng Hao-Ying Hsieh Ching-Wen Tsai An-Li Lee Chien-Yi Lu Yin-Tzu Chen Tai-Horng Young pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces Materials Today Bio pH-responsive surface Polyamide-66/chitosan Continuous cell production SDF-1/CXCR4 axis Cell therapy |
| title | pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces |
| title_full | pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces |
| title_fullStr | pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces |
| title_full_unstemmed | pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces |
| title_short | pH-driven continuous stem cell production with enhanced regenerative capacity from polyamide/chitosan surfaces |
| title_sort | ph driven continuous stem cell production with enhanced regenerative capacity from polyamide chitosan surfaces |
| topic | pH-responsive surface Polyamide-66/chitosan Continuous cell production SDF-1/CXCR4 axis Cell therapy |
| url | http://www.sciencedirect.com/science/article/pii/S259000642200312X |
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