Organoid assessment technologies
Abstract Despite enormous advances in the generation of organoids, robust and stable protocols of organoids are still a major challenge to researchers. Research for assessing structures of organoids and the evaluations of their functions on in vitro or in vivo is often limited by precision strategie...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-12-01
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| Series: | Clinical and Translational Medicine |
| Online Access: | https://doi.org/10.1002/ctm2.1499 |
| _version_ | 1797374989589020672 |
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| author | Yuyuan Gu Wencai Zhang Xianmin Wu Yuanwei Zhang Ke Xu Jiacan Su |
| author_facet | Yuyuan Gu Wencai Zhang Xianmin Wu Yuanwei Zhang Ke Xu Jiacan Su |
| author_sort | Yuyuan Gu |
| collection | DOAJ |
| description | Abstract Despite enormous advances in the generation of organoids, robust and stable protocols of organoids are still a major challenge to researchers. Research for assessing structures of organoids and the evaluations of their functions on in vitro or in vivo is often limited by precision strategies. A growing interest in assessing organoids has arisen, aimed at standardizing the process of obtaining organoids to accurately resemble human‐derived tissue. The complex microenvironment of organoids, intricate cellular crosstalk, organ‐specific architectures and further complicate functions urgently quest for high‐through schemes. By utilizing multi‐omics analysis and single‐cell analysis, cell‐cell interaction mechanisms can be deciphered, and their structures can be investigated in a detailed view by histological analysis. In this review, we will conclude the novel approaches to study the molecular mechanism and cell heterogeneity of organoids and discuss the histological and morphological similarity of organoids in comparison to the human body. Future perspectives on functional analysis will be developed and the organoids will become mature models. |
| first_indexed | 2024-03-08T19:15:43Z |
| format | Article |
| id | doaj.art-986502aadd72426b8526ccb10ce5741a |
| institution | Directory Open Access Journal |
| issn | 2001-1326 |
| language | English |
| last_indexed | 2024-03-08T19:15:43Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Medicine |
| spelling | doaj.art-986502aadd72426b8526ccb10ce5741a2023-12-27T07:30:35ZengWileyClinical and Translational Medicine2001-13262023-12-011312n/an/a10.1002/ctm2.1499Organoid assessment technologiesYuyuan Gu0Wencai Zhang1Xianmin Wu2Yuanwei Zhang3Ke Xu4Jiacan Su5Institute of Translational Medicine Shanghai University Shanghai ChinaDepartment of Orthopedics First Affiliated Hospital Jinan University Guangzhou ChinaDepartment of Orthopedics Shanghai Zhongye Hospital Shanghai ChinaInstitute of Translational Medicine Shanghai University Shanghai ChinaInstitute of Translational Medicine Shanghai University Shanghai ChinaInstitute of Translational Medicine Shanghai University Shanghai ChinaAbstract Despite enormous advances in the generation of organoids, robust and stable protocols of organoids are still a major challenge to researchers. Research for assessing structures of organoids and the evaluations of their functions on in vitro or in vivo is often limited by precision strategies. A growing interest in assessing organoids has arisen, aimed at standardizing the process of obtaining organoids to accurately resemble human‐derived tissue. The complex microenvironment of organoids, intricate cellular crosstalk, organ‐specific architectures and further complicate functions urgently quest for high‐through schemes. By utilizing multi‐omics analysis and single‐cell analysis, cell‐cell interaction mechanisms can be deciphered, and their structures can be investigated in a detailed view by histological analysis. In this review, we will conclude the novel approaches to study the molecular mechanism and cell heterogeneity of organoids and discuss the histological and morphological similarity of organoids in comparison to the human body. Future perspectives on functional analysis will be developed and the organoids will become mature models.https://doi.org/10.1002/ctm2.1499 |
| spellingShingle | Yuyuan Gu Wencai Zhang Xianmin Wu Yuanwei Zhang Ke Xu Jiacan Su Organoid assessment technologies Clinical and Translational Medicine |
| title | Organoid assessment technologies |
| title_full | Organoid assessment technologies |
| title_fullStr | Organoid assessment technologies |
| title_full_unstemmed | Organoid assessment technologies |
| title_short | Organoid assessment technologies |
| title_sort | organoid assessment technologies |
| url | https://doi.org/10.1002/ctm2.1499 |
| work_keys_str_mv | AT yuyuangu organoidassessmenttechnologies AT wencaizhang organoidassessmenttechnologies AT xianminwu organoidassessmenttechnologies AT yuanweizhang organoidassessmenttechnologies AT kexu organoidassessmenttechnologies AT jiacansu organoidassessmenttechnologies |