Superoxide Radical Generation Mediated Plasmodium berghei Infection in Swiss Mice
Aim: This study was aimed to evaluate the duration dependent study to develop Plasmodium berghei infection in in vivo system. Methods: 200µl of infected blood containing 1X105 parasites was challenged in Swiss mice intraperitoneally for 5, 10 and 15 days. After sacrifice, parasitemia, O2.- generatio...
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| Format: | Article |
| Language: | English |
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Al Ameen Medical College
2012-01-01
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| Series: | Al Ameen Journal of Medical Sciences |
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| Online Access: | http://ajms.alameenmedical.org/article_vol05-1-jan-mar-2012/AJMS.5.1.2012%20P%2069-81.pdf |
| _version_ | 1818985818750976000 |
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| author | Satyajit Tripathy, Subhankari Prasad Chakraborty and Somenath Roy |
| author_facet | Satyajit Tripathy, Subhankari Prasad Chakraborty and Somenath Roy |
| author_sort | Satyajit Tripathy, Subhankari Prasad Chakraborty and Somenath Roy |
| collection | DOAJ |
| description | Aim: This study was aimed to evaluate the duration dependent study to develop Plasmodium berghei infection in in vivo system. Methods: 200µl of infected blood containing 1X105 parasites was challenged in Swiss mice intraperitoneally for 5, 10 and 15 days. After sacrifice, parasitemia, O2.- generation, NADPH oxidase activity, NO generation of lymphocyte; MDA level, TrxR activity and haemolytic activity of infected RBC; activities of LDH, CRP level, SGOT and SGPT, NO release of serum; the MPO activity of serum, liver, spleen and kidney were studied. Results: O2.- generation, NADPH oxidase activity, NO generation of lymphocyte; LDH activity, CRP, SGOT, SGPT level and NO release of serum and TrxR, haemolytic activity were significantly increased than control; and also MDA level of infected RBC significantly increased as compared to control. These effects were the maximum after 10 days of challenge. Conclusion: The findings suggest that, P. berghei NK65 infection maximally develop after 10 days infection in Swiss mice. |
| first_indexed | 2024-12-20T18:40:57Z |
| format | Article |
| id | doaj.art-9865ee4611614975bacad811ed7e9e3d |
| institution | Directory Open Access Journal |
| issn | 0974-1143 |
| language | English |
| last_indexed | 2024-12-20T18:40:57Z |
| publishDate | 2012-01-01 |
| publisher | Al Ameen Medical College |
| record_format | Article |
| series | Al Ameen Journal of Medical Sciences |
| spelling | doaj.art-9865ee4611614975bacad811ed7e9e3d2022-12-21T19:29:48ZengAl Ameen Medical CollegeAl Ameen Journal of Medical Sciences0974-11432012-01-0105016981Superoxide Radical Generation Mediated Plasmodium berghei Infection in Swiss MiceSatyajit Tripathy, Subhankari Prasad Chakraborty and Somenath RoyAim: This study was aimed to evaluate the duration dependent study to develop Plasmodium berghei infection in in vivo system. Methods: 200µl of infected blood containing 1X105 parasites was challenged in Swiss mice intraperitoneally for 5, 10 and 15 days. After sacrifice, parasitemia, O2.- generation, NADPH oxidase activity, NO generation of lymphocyte; MDA level, TrxR activity and haemolytic activity of infected RBC; activities of LDH, CRP level, SGOT and SGPT, NO release of serum; the MPO activity of serum, liver, spleen and kidney were studied. Results: O2.- generation, NADPH oxidase activity, NO generation of lymphocyte; LDH activity, CRP, SGOT, SGPT level and NO release of serum and TrxR, haemolytic activity were significantly increased than control; and also MDA level of infected RBC significantly increased as compared to control. These effects were the maximum after 10 days of challenge. Conclusion: The findings suggest that, P. berghei NK65 infection maximally develop after 10 days infection in Swiss mice.http://ajms.alameenmedical.org/article_vol05-1-jan-mar-2012/AJMS.5.1.2012%20P%2069-81.pdfMalariaPlasmodium bergheiLDHsuperoxide radicalNADPH oxidasethioredoxin reductase |
| spellingShingle | Satyajit Tripathy, Subhankari Prasad Chakraborty and Somenath Roy Superoxide Radical Generation Mediated Plasmodium berghei Infection in Swiss Mice Al Ameen Journal of Medical Sciences Malaria Plasmodium berghei LDH superoxide radical NADPH oxidase thioredoxin reductase |
| title | Superoxide Radical Generation Mediated Plasmodium berghei Infection in Swiss Mice |
| title_full | Superoxide Radical Generation Mediated Plasmodium berghei Infection in Swiss Mice |
| title_fullStr | Superoxide Radical Generation Mediated Plasmodium berghei Infection in Swiss Mice |
| title_full_unstemmed | Superoxide Radical Generation Mediated Plasmodium berghei Infection in Swiss Mice |
| title_short | Superoxide Radical Generation Mediated Plasmodium berghei Infection in Swiss Mice |
| title_sort | superoxide radical generation mediated plasmodium berghei infection in swiss mice |
| topic | Malaria Plasmodium berghei LDH superoxide radical NADPH oxidase thioredoxin reductase |
| url | http://ajms.alameenmedical.org/article_vol05-1-jan-mar-2012/AJMS.5.1.2012%20P%2069-81.pdf |
| work_keys_str_mv | AT satyajittripathysubhankariprasadchakrabortyandsomenathroy superoxideradicalgenerationmediatedplasmodiumbergheiinfectioninswissmice |