High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
Abstract Background Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. Results We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification pr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-018-5299-0 |
| _version_ | 1818601435559886848 |
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| author | Charlotte Gustafsson Ayla De Paepe Christian Schmidl Robert Månsson |
| author_facet | Charlotte Gustafsson Ayla De Paepe Christian Schmidl Robert Månsson |
| author_sort | Charlotte Gustafsson |
| collection | DOAJ |
| description | Abstract Background Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. Results We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes. Conclusions The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation. |
| first_indexed | 2024-12-16T12:51:20Z |
| format | Article |
| id | doaj.art-9867ea83141d4afda198d447edd55d42 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-16T12:51:20Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-9867ea83141d4afda198d447edd55d422022-12-21T22:31:09ZengBMCBMC Genomics1471-21642019-01-012011610.1186/s12864-018-5299-0High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbersCharlotte Gustafsson0Ayla De Paepe1Christian Schmidl2Robert Månsson3Center for Hematology and Regenerative Medicine Huddinge, Karolinska InstitutetCenter for Hematology and Regenerative Medicine Huddinge, Karolinska InstitutetRegensburg Centre for Interventional Immunology (RCI) and University Medical CenterCenter for Hematology and Regenerative Medicine Huddinge, Karolinska InstitutetAbstract Background Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. Results We present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes. Conclusions The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.http://link.springer.com/article/10.1186/s12864-018-5299-0Chromatin immunoprecipitationChIP-seqChIPmentationHigh-throughput genomicsEpigenetics |
| spellingShingle | Charlotte Gustafsson Ayla De Paepe Christian Schmidl Robert Månsson High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers BMC Genomics Chromatin immunoprecipitation ChIP-seq ChIPmentation High-throughput genomics Epigenetics |
| title | High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers |
| title_full | High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers |
| title_fullStr | High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers |
| title_full_unstemmed | High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers |
| title_short | High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers |
| title_sort | high throughput chipmentation freely scalable single day chipseq data generation from very low cell numbers |
| topic | Chromatin immunoprecipitation ChIP-seq ChIPmentation High-throughput genomics Epigenetics |
| url | http://link.springer.com/article/10.1186/s12864-018-5299-0 |
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