Genotyping of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi from sheep and goats in China
Abstract Background Few studies have molecularly characterized the potential zoonotic protozoa, Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in sheep and goats in China, therefore total 472 fecal samples were collected from eight provinces and infection rates of three protozo...
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-022-03447-6 |
| _version_ | 1828108293102370816 |
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| author | Penglin Wang Ling Zheng Linke Liu Fuchang Yu Yichen Jian Rongjun Wang Sumei Zhang Longxian Zhang Changshen Ning Fuchun Jian |
| author_facet | Penglin Wang Ling Zheng Linke Liu Fuchang Yu Yichen Jian Rongjun Wang Sumei Zhang Longxian Zhang Changshen Ning Fuchun Jian |
| author_sort | Penglin Wang |
| collection | DOAJ |
| description | Abstract Background Few studies have molecularly characterized the potential zoonotic protozoa, Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in sheep and goats in China, therefore total 472 fecal samples were collected from eight provinces and infection rates of three protozoa were determined by PCR analysis of corresponding loci. All PCR positive samples were sequenced to identify the genotype. Results The overall infection rates for Cryptosporidium, G. duodenalis, and E. bieneusi were 1.9% (9/472), 20.6% (97/472), and 44.5% (210/472), respectively. C. xiaoi (n = 5), C. ubiquitum (n = 3), and C. anderson (n = 1) were identified in goats. 97 G. duodenalis strains were successfully detected, and assembly E (n = 96) and assembly A (n = 1) were identified. Two novel G. duodenalis multilocus genotype (MLGs) were identified, with one belonging to subgroup AI and the other to subgroup E5. Nine known genotype (BEB6, CD6, CHC8, CHG3, CHG5, Peru6, CHG1, CHG2, and COS-I) and four new genotype (CHG26, CHG27, CHG28, and CHS18) were identified in E. bieneusi, with CHG3 dominant in this group. Conclusions The present results highlight the role of sheep and goats as reservoir hosts for this three gastrointestinal pathogens. In summary, we provided a platform for more detailed research on genotyping or subtyping intestinal pathogens to better understand their risks and modes of transmission. |
| first_indexed | 2024-04-11T10:44:36Z |
| format | Article |
| id | doaj.art-986920755f2a4cda9a42ece6aa102c1b |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-11T10:44:36Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-986920755f2a4cda9a42ece6aa102c1b2022-12-22T04:29:06ZengBMCBMC Veterinary Research1746-61482022-09-0118111110.1186/s12917-022-03447-6Genotyping of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi from sheep and goats in ChinaPenglin Wang0Ling Zheng1Linke Liu2Fuchang Yu3Yichen Jian4Rongjun Wang5Sumei Zhang6Longxian Zhang7Changshen Ning8Fuchun Jian9College of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityCollege of Veterinary Medicine, Henan Agricultural UniversityAbstract Background Few studies have molecularly characterized the potential zoonotic protozoa, Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in sheep and goats in China, therefore total 472 fecal samples were collected from eight provinces and infection rates of three protozoa were determined by PCR analysis of corresponding loci. All PCR positive samples were sequenced to identify the genotype. Results The overall infection rates for Cryptosporidium, G. duodenalis, and E. bieneusi were 1.9% (9/472), 20.6% (97/472), and 44.5% (210/472), respectively. C. xiaoi (n = 5), C. ubiquitum (n = 3), and C. anderson (n = 1) were identified in goats. 97 G. duodenalis strains were successfully detected, and assembly E (n = 96) and assembly A (n = 1) were identified. Two novel G. duodenalis multilocus genotype (MLGs) were identified, with one belonging to subgroup AI and the other to subgroup E5. Nine known genotype (BEB6, CD6, CHC8, CHG3, CHG5, Peru6, CHG1, CHG2, and COS-I) and four new genotype (CHG26, CHG27, CHG28, and CHS18) were identified in E. bieneusi, with CHG3 dominant in this group. Conclusions The present results highlight the role of sheep and goats as reservoir hosts for this three gastrointestinal pathogens. In summary, we provided a platform for more detailed research on genotyping or subtyping intestinal pathogens to better understand their risks and modes of transmission.https://doi.org/10.1186/s12917-022-03447-6Cryptosporidium spp.Giardia duodenalisEnterocytozoon bieneusiSheepGoatsGenotype |
| spellingShingle | Penglin Wang Ling Zheng Linke Liu Fuchang Yu Yichen Jian Rongjun Wang Sumei Zhang Longxian Zhang Changshen Ning Fuchun Jian Genotyping of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi from sheep and goats in China BMC Veterinary Research Cryptosporidium spp. Giardia duodenalis Enterocytozoon bieneusi Sheep Goats Genotype |
| title | Genotyping of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi from sheep and goats in China |
| title_full | Genotyping of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi from sheep and goats in China |
| title_fullStr | Genotyping of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi from sheep and goats in China |
| title_full_unstemmed | Genotyping of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi from sheep and goats in China |
| title_short | Genotyping of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi from sheep and goats in China |
| title_sort | genotyping of cryptosporidium spp giardia duodenalis and enterocytozoon bieneusi from sheep and goats in china |
| topic | Cryptosporidium spp. Giardia duodenalis Enterocytozoon bieneusi Sheep Goats Genotype |
| url | https://doi.org/10.1186/s12917-022-03447-6 |
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