New potential biomarkers for early chronic kidney disease diagnosis in patients with different glucose tolerance status
BackgroundThe purpose of the present study was to investigate the role of oxidative stress, platelet activation, and endocan levels in renal dysfunction in normal glucose tolerance (NGT) patients with 1-h plasma glucose values ≥155 mg/dl (NGT ≥ 155), compared to NGT < 155, impaired glucose to...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2023.1206336/full |
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| author | Velia Cassano Corrado Pelaia Giuseppe Armentaro Sofia Miceli Valeria Tallarico Daniele Dallimonti Perini Vanessa T. Fiorentino Egidio Imbalzano Raffaele Maio Elena Succurro Marta L. Hribal Francesco Andreozzi Giorgio Sesti Angela Sciacqua |
| author_facet | Velia Cassano Corrado Pelaia Giuseppe Armentaro Sofia Miceli Valeria Tallarico Daniele Dallimonti Perini Vanessa T. Fiorentino Egidio Imbalzano Raffaele Maio Elena Succurro Marta L. Hribal Francesco Andreozzi Giorgio Sesti Angela Sciacqua |
| author_sort | Velia Cassano |
| collection | DOAJ |
| description | BackgroundThe purpose of the present study was to investigate the role of oxidative stress, platelet activation, and endocan levels in renal dysfunction in normal glucose tolerance (NGT) patients with 1-h plasma glucose values ≥155 mg/dl (NGT ≥ 155), compared to NGT < 155, impaired glucose tolerance (IGT), and type 2 diabetes mellitus (T2DM) newly diagnosed subjects. We enlisted 233 patients subjected to an oral glucose tolerance test (OGTT).Materials and methodsThe serum levels of platelet activation (glycoprotein VI and sP-selectin), oxidative stress biomarkers (8-isoprostane and Nox-2), and endocan were evaluated using an ELISA test.ResultsAmong NGT < 155 patients and the T2DM group, there was a statistically significant increase in 8-isoprostane (p < 0.0001), Nox-2 (p < 0.0001), glycoprotein VI (p < 0.0001), and sP-selectin (p < 0.0001) serum levels. Higher serum endocan levels were found with the worsening of metabolic profile (p < 0.0001); specifically, NGT ≥ 155 patients presented higher serum endocan values when compared to NGT < 155 patients (p < 0.0001). From the multivariate linear regression analysis, 1-h glucose resulted in the major predictor of estimated glomerular filtration rate (e-GFR) justifying 23.6% of its variation (p < 0.0001); 8-isoprostane and Nox-2 added respectively another 6.0% (p < 0.0001) and 3.2% (p = 0.001).ConclusionOur study confirmed the link between 1-h post-load glucose ≥155 mg/dl during OGTT and the possible increased risk for chronic kidney disease (CKD) in newly diagnosed patients. The novelty is that we demonstrated a progressive increase in oxidative stress, platelet activation, and serum endocan levels with the worsening of metabolic profile, which becomes evident early during the progression of CKD. |
| first_indexed | 2024-03-13T00:58:16Z |
| format | Article |
| id | doaj.art-986b5bca16e841d9b94cbd3aedf5d26c |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-03-13T00:58:16Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-986b5bca16e841d9b94cbd3aedf5d26c2023-07-06T15:24:16ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-07-011410.3389/fendo.2023.12063361206336New potential biomarkers for early chronic kidney disease diagnosis in patients with different glucose tolerance statusVelia Cassano0Corrado Pelaia1Giuseppe Armentaro2Sofia Miceli3Valeria Tallarico4Daniele Dallimonti Perini5Vanessa T. Fiorentino6Egidio Imbalzano7Raffaele Maio8Elena Succurro9Marta L. Hribal10Francesco Andreozzi11Giorgio Sesti12Angela Sciacqua13Department of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Experimental and Clinical Medicine, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Clinical and Experimental Medicine, Polyclinic University of Messina, Messina, ItalyDepartment of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyDepartment of Clinical and Molecular Medicine, Sapienza University of Rome, Rome, ItalyDepartment of Medical and Surgical Sciences, University Magna Græcia of Catanzaro, Catanzaro, ItalyBackgroundThe purpose of the present study was to investigate the role of oxidative stress, platelet activation, and endocan levels in renal dysfunction in normal glucose tolerance (NGT) patients with 1-h plasma glucose values ≥155 mg/dl (NGT ≥ 155), compared to NGT < 155, impaired glucose tolerance (IGT), and type 2 diabetes mellitus (T2DM) newly diagnosed subjects. We enlisted 233 patients subjected to an oral glucose tolerance test (OGTT).Materials and methodsThe serum levels of platelet activation (glycoprotein VI and sP-selectin), oxidative stress biomarkers (8-isoprostane and Nox-2), and endocan were evaluated using an ELISA test.ResultsAmong NGT < 155 patients and the T2DM group, there was a statistically significant increase in 8-isoprostane (p < 0.0001), Nox-2 (p < 0.0001), glycoprotein VI (p < 0.0001), and sP-selectin (p < 0.0001) serum levels. Higher serum endocan levels were found with the worsening of metabolic profile (p < 0.0001); specifically, NGT ≥ 155 patients presented higher serum endocan values when compared to NGT < 155 patients (p < 0.0001). From the multivariate linear regression analysis, 1-h glucose resulted in the major predictor of estimated glomerular filtration rate (e-GFR) justifying 23.6% of its variation (p < 0.0001); 8-isoprostane and Nox-2 added respectively another 6.0% (p < 0.0001) and 3.2% (p = 0.001).ConclusionOur study confirmed the link between 1-h post-load glucose ≥155 mg/dl during OGTT and the possible increased risk for chronic kidney disease (CKD) in newly diagnosed patients. The novelty is that we demonstrated a progressive increase in oxidative stress, platelet activation, and serum endocan levels with the worsening of metabolic profile, which becomes evident early during the progression of CKD.https://www.frontiersin.org/articles/10.3389/fendo.2023.1206336/fulloxidative stressrenal functionglucose toleranceendocanplatelets activation |
| spellingShingle | Velia Cassano Corrado Pelaia Giuseppe Armentaro Sofia Miceli Valeria Tallarico Daniele Dallimonti Perini Vanessa T. Fiorentino Egidio Imbalzano Raffaele Maio Elena Succurro Marta L. Hribal Francesco Andreozzi Giorgio Sesti Angela Sciacqua New potential biomarkers for early chronic kidney disease diagnosis in patients with different glucose tolerance status Frontiers in Endocrinology oxidative stress renal function glucose tolerance endocan platelets activation |
| title | New potential biomarkers for early chronic kidney disease diagnosis in patients with different glucose tolerance status |
| title_full | New potential biomarkers for early chronic kidney disease diagnosis in patients with different glucose tolerance status |
| title_fullStr | New potential biomarkers for early chronic kidney disease diagnosis in patients with different glucose tolerance status |
| title_full_unstemmed | New potential biomarkers for early chronic kidney disease diagnosis in patients with different glucose tolerance status |
| title_short | New potential biomarkers for early chronic kidney disease diagnosis in patients with different glucose tolerance status |
| title_sort | new potential biomarkers for early chronic kidney disease diagnosis in patients with different glucose tolerance status |
| topic | oxidative stress renal function glucose tolerance endocan platelets activation |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2023.1206336/full |
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