The effect of high-frequency neuromuscular electrical stimulation training on skeletal muscle properties in mice
The aim of this study was to analyze the effects of high-frequency neuromuscular electrical stimulation training (NMES) on the structure, function and oxidative capacity of the skeletal muscle using a mice model (C57BL/6J strain, n=8). The left tibialis anterior muscle in mice was electr...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2017-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600110V.pdf |
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| author | Valenzuela Pedro L. de Melo Aroeira Andresa E. Torrella Joan R. de la Villa Pedro |
| author_facet | Valenzuela Pedro L. de Melo Aroeira Andresa E. Torrella Joan R. de la Villa Pedro |
| author_sort | Valenzuela Pedro L. |
| collection | DOAJ |
| description | The aim of this study was to analyze the effects of high-frequency
neuromuscular electrical stimulation training (NMES) on the structure,
function and oxidative capacity of the skeletal muscle using a mice model
(C57BL/6J strain, n=8). The left tibialis anterior muscle in mice was
electro-stimulated (ST) whereas the right muscle was maintained as an
internal control (CT). The ST limb was submitted to eight surface (100 Hz)
NMES sessions in two weeks, with a minimum gap of 24 h between sessions. NMES
training increased muscle mass (42.0±3.3 vs. 36.1±5.4 mg, p<0.05, effect size
[ES] r=0.55), the mean fiber cross-sectional area (FCSA) (3318±333 vs.
2577±405 μ2, p<0.001, ES=0.71), maximal force (224.7±13.8 vs. 184.5±30.9 mN,
p<0.01, ES=0.64), and the rate of force development (1.63±0.14 vs. 1.34±0.20
mN/ms, p<0.05, ES=0.64), with no effects on the muscle oxidative profile.
These results demonstrate that surface NMES induced muscle hypertrophy and
instigated an improvement in the contractile properties of the TA muscle in
mice. Therefore, this animal model appears to be suitable for the study of
hypertrophic processes as it enables better control of the stimulus
properties (intensity, duration, frequency, etc.) than other traditionally
used animal models and does not require negative reinforcements or surgical
procedures. |
| first_indexed | 2024-12-23T05:33:21Z |
| format | Article |
| id | doaj.art-986ff74b77be438db1b12257eaf28bb1 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-23T05:33:21Z |
| publishDate | 2017-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-986ff74b77be438db1b12257eaf28bb12022-12-21T17:58:24ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392017-01-0169339139710.2298/ABS160925110V0354-46641600110VThe effect of high-frequency neuromuscular electrical stimulation training on skeletal muscle properties in miceValenzuela Pedro L.0de Melo Aroeira Andresa E.1Torrella Joan R.2de la Villa Pedro3University of Alcalá, School of Medicine, Department of Systems Biology, Physiology Unit, SpainUniversity of Alcalá, School of Medicine, Department of Systems Biology, Physiology Unit, SpainUniversity of Barcelona, Faculty of Biology, Department of Physiology and Immunology, Barselona, SpainUniversity of Alcalá, School of Medicine, Department of Systems Biology, Physiology Unit, SpainThe aim of this study was to analyze the effects of high-frequency neuromuscular electrical stimulation training (NMES) on the structure, function and oxidative capacity of the skeletal muscle using a mice model (C57BL/6J strain, n=8). The left tibialis anterior muscle in mice was electro-stimulated (ST) whereas the right muscle was maintained as an internal control (CT). The ST limb was submitted to eight surface (100 Hz) NMES sessions in two weeks, with a minimum gap of 24 h between sessions. NMES training increased muscle mass (42.0±3.3 vs. 36.1±5.4 mg, p<0.05, effect size [ES] r=0.55), the mean fiber cross-sectional area (FCSA) (3318±333 vs. 2577±405 μ2, p<0.001, ES=0.71), maximal force (224.7±13.8 vs. 184.5±30.9 mN, p<0.01, ES=0.64), and the rate of force development (1.63±0.14 vs. 1.34±0.20 mN/ms, p<0.05, ES=0.64), with no effects on the muscle oxidative profile. These results demonstrate that surface NMES induced muscle hypertrophy and instigated an improvement in the contractile properties of the TA muscle in mice. Therefore, this animal model appears to be suitable for the study of hypertrophic processes as it enables better control of the stimulus properties (intensity, duration, frequency, etc.) than other traditionally used animal models and does not require negative reinforcements or surgical procedures.http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600110V.pdfskeletal muscleneuromuscular electrical stimulationmuscle masshypertrophymuscle force |
| spellingShingle | Valenzuela Pedro L. de Melo Aroeira Andresa E. Torrella Joan R. de la Villa Pedro The effect of high-frequency neuromuscular electrical stimulation training on skeletal muscle properties in mice Archives of Biological Sciences skeletal muscle neuromuscular electrical stimulation muscle mass hypertrophy muscle force |
| title | The effect of high-frequency neuromuscular electrical stimulation training on skeletal muscle properties in mice |
| title_full | The effect of high-frequency neuromuscular electrical stimulation training on skeletal muscle properties in mice |
| title_fullStr | The effect of high-frequency neuromuscular electrical stimulation training on skeletal muscle properties in mice |
| title_full_unstemmed | The effect of high-frequency neuromuscular electrical stimulation training on skeletal muscle properties in mice |
| title_short | The effect of high-frequency neuromuscular electrical stimulation training on skeletal muscle properties in mice |
| title_sort | effect of high frequency neuromuscular electrical stimulation training on skeletal muscle properties in mice |
| topic | skeletal muscle neuromuscular electrical stimulation muscle mass hypertrophy muscle force |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600110V.pdf |
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