Targeted microbubbles combined with low-power focused ultrasound promote the thrombolysis of acute deep vein thrombosis
Introduction: The side effects of conventional therapy for acute deep vein thrombosis (DVT) are severe, with inflammatory reactions playing a pivotal role. It is particularly important to explore new ways of treatment thrombosis by targeting inflammatory factors.Methods: A targeted microbubble contr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1163405/full |
| _version_ | 1797869636420632576 |
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| author | Jianfu Chen Yuan Yang Yunyan Li Lirong Xu Chun Zhao Qi Chen Yongping Lu |
| author_facet | Jianfu Chen Yuan Yang Yunyan Li Lirong Xu Chun Zhao Qi Chen Yongping Lu |
| author_sort | Jianfu Chen |
| collection | DOAJ |
| description | Introduction: The side effects of conventional therapy for acute deep vein thrombosis (DVT) are severe, with inflammatory reactions playing a pivotal role. It is particularly important to explore new ways of treatment thrombosis by targeting inflammatory factors.Methods: A targeted microbubble contrast agent was prepared using the biotin-avidin method. The 40 DVT model rabbits were established and divided into four groups according to different treatment regimens. The four coagulation indexes, TNF-α, and D-dimer content of experimental animals were measured before modeling and before and after treatment, and the thrombolysis was assessed by ultrasound imaging. Finally, the results were verified by pathology.Results and Discussion: Fluorescence microscopy verified the successful preparation of targeted microbubbles. Among the groups, PT, APTT, and TT in Group II-IV were longer than those in Group I (all p < 0.05). FIB and D-dimer content were lower than those in Group I (all p < 0.05), and TNF-α content in Group IV was lower than that in Group I-III (all p < 0.05). Pairwise comparison before modeling and before treatment and after treatment showed that, after treatment, the PT, APTT, and TT in Group II-IV were longer than those before modeling (all p < 0.05). The contents of FIB and D-dimer were lower than those before modeling and before treatment (all p < 0.05). The content of TNF-α decreased significantly only in Group IV, but increased in the other three groups. Targeted microbubbles combined with Low-power focused ultrasound can reduce inflammation, significantly promote thrombolysis, and provide new ideas and methods for the diagnosis and treatment of acute DVT. |
| first_indexed | 2024-04-10T00:14:39Z |
| format | Article |
| id | doaj.art-9876bd11d51e484b853d71607bd2b97d |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T00:14:39Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-9876bd11d51e484b853d71607bd2b97d2023-03-16T06:06:46ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-03-011110.3389/fbioe.2023.11634051163405Targeted microbubbles combined with low-power focused ultrasound promote the thrombolysis of acute deep vein thrombosisJianfu Chen0Yuan Yang1Yunyan Li2Lirong Xu3Chun Zhao4Qi Chen5Yongping Lu6Kunming Medical University, Kunming, Yunnan, ChinaKunming Medical University, Kunming, Yunnan, ChinaDepartment of Ultrasound, The Affiliated Hospital of Yunnan University (The Second People’s Hospital of Yunnan Province), Kunming, Yunnan, ChinaDepartment of Ultrasound, The Affiliated Hospital of Yunnan University (The Second People’s Hospital of Yunnan Province), Kunming, Yunnan, ChinaDepartment of Ultrasound, The Affiliated Hospital of Yunnan University (The Second People’s Hospital of Yunnan Province), Kunming, Yunnan, ChinaSchool of Clinical Medicine, Dali University, Dali, Yunnan, ChinaDepartment of Ultrasound, The Affiliated Hospital of Yunnan University (The Second People’s Hospital of Yunnan Province), Kunming, Yunnan, ChinaIntroduction: The side effects of conventional therapy for acute deep vein thrombosis (DVT) are severe, with inflammatory reactions playing a pivotal role. It is particularly important to explore new ways of treatment thrombosis by targeting inflammatory factors.Methods: A targeted microbubble contrast agent was prepared using the biotin-avidin method. The 40 DVT model rabbits were established and divided into four groups according to different treatment regimens. The four coagulation indexes, TNF-α, and D-dimer content of experimental animals were measured before modeling and before and after treatment, and the thrombolysis was assessed by ultrasound imaging. Finally, the results were verified by pathology.Results and Discussion: Fluorescence microscopy verified the successful preparation of targeted microbubbles. Among the groups, PT, APTT, and TT in Group II-IV were longer than those in Group I (all p < 0.05). FIB and D-dimer content were lower than those in Group I (all p < 0.05), and TNF-α content in Group IV was lower than that in Group I-III (all p < 0.05). Pairwise comparison before modeling and before treatment and after treatment showed that, after treatment, the PT, APTT, and TT in Group II-IV were longer than those before modeling (all p < 0.05). The contents of FIB and D-dimer were lower than those before modeling and before treatment (all p < 0.05). The content of TNF-α decreased significantly only in Group IV, but increased in the other three groups. Targeted microbubbles combined with Low-power focused ultrasound can reduce inflammation, significantly promote thrombolysis, and provide new ideas and methods for the diagnosis and treatment of acute DVT.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1163405/fulltargeted microbubbleslow-power focused ultrasoundultrasonic irradiationacute deep vein thrombosisthrombolysis |
| spellingShingle | Jianfu Chen Yuan Yang Yunyan Li Lirong Xu Chun Zhao Qi Chen Yongping Lu Targeted microbubbles combined with low-power focused ultrasound promote the thrombolysis of acute deep vein thrombosis Frontiers in Bioengineering and Biotechnology targeted microbubbles low-power focused ultrasound ultrasonic irradiation acute deep vein thrombosis thrombolysis |
| title | Targeted microbubbles combined with low-power focused ultrasound promote the thrombolysis of acute deep vein thrombosis |
| title_full | Targeted microbubbles combined with low-power focused ultrasound promote the thrombolysis of acute deep vein thrombosis |
| title_fullStr | Targeted microbubbles combined with low-power focused ultrasound promote the thrombolysis of acute deep vein thrombosis |
| title_full_unstemmed | Targeted microbubbles combined with low-power focused ultrasound promote the thrombolysis of acute deep vein thrombosis |
| title_short | Targeted microbubbles combined with low-power focused ultrasound promote the thrombolysis of acute deep vein thrombosis |
| title_sort | targeted microbubbles combined with low power focused ultrasound promote the thrombolysis of acute deep vein thrombosis |
| topic | targeted microbubbles low-power focused ultrasound ultrasonic irradiation acute deep vein thrombosis thrombolysis |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1163405/full |
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