Use of calcium alginate/biochar microsphere immobilized bacteria Bacillus sp. for removal of phenol in water
A highly efficient phenol-degrading bacteria strain, identified as Bacillus cereus (GenBank No:MN784421) by 16S rDNA analysis, was used for phenol treatment in the water. Under the optimal degradation conditions (10% inoculation of the strain suspension into beef extract peptone medium; 35 °C; pH 7....
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Environmental Challenges |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266701002200155X |
| _version_ | 1811206985112092672 |
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| author | Jian Li Yinjuan Jia Jiaochan Zhong Qinghui Liu Han Li Igor Agranovski |
| author_facet | Jian Li Yinjuan Jia Jiaochan Zhong Qinghui Liu Han Li Igor Agranovski |
| author_sort | Jian Li |
| collection | DOAJ |
| description | A highly efficient phenol-degrading bacteria strain, identified as Bacillus cereus (GenBank No:MN784421) by 16S rDNA analysis, was used for phenol treatment in the water. Under the optimal degradation conditions (10% inoculation of the strain suspension into beef extract peptone medium; 35 °C; pH 7.0; 1% NaCl), the bacterial strain provided 97.71% degradation rate within 46 h of treatment. For improving the tolerance of the strain to phenol toxicity, water hyacinth (Eichhornia crassipes) was used as a raw material to produce a biochar under different pyrolysis temperatures. It has been shown that treatment of 1400 mg/L phenol by a combination of a bacterial strain and 0.6% (w/v) biochar prepared at 550 °C can achieve a degradation rate of 99.5% within 60 h of treatment. It has also been shown that inoculated biochar, embedded and immobilized by calcium alginate to form calcium alginate/biochar microbial microsphere (CABMM with 3-4 mm diameter), could greatly improve the tolerance of bacteria to phenol toxicity. At the initial phenol concentration of 1400 mg/L, using of CABMM enables to achieve 99.1% of the phenol degradation over 40 h of treatment. |
| first_indexed | 2024-04-12T03:57:20Z |
| format | Article |
| id | doaj.art-987719118b1d4a9c9c5378302ad00f55 |
| institution | Directory Open Access Journal |
| issn | 2667-0100 |
| language | English |
| last_indexed | 2024-04-12T03:57:20Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environmental Challenges |
| spelling | doaj.art-987719118b1d4a9c9c5378302ad00f552022-12-22T03:48:48ZengElsevierEnvironmental Challenges2667-01002022-12-019100599Use of calcium alginate/biochar microsphere immobilized bacteria Bacillus sp. for removal of phenol in waterJian Li0Yinjuan Jia1Jiaochan Zhong2Qinghui Liu3Han Li4Igor Agranovski5School of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang 330063, China; School of Engineering and Built Environment, Griffith University, Brisbane, QLD 4111, AustraliaSchool of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang 330063, ChinaSchool of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang 330063, ChinaSchool of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang 330063, ChinaSchool of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang 330063, ChinaSchool of Engineering and Built Environment, Griffith University, Brisbane, QLD 4111, Australia; Corresponding author.A highly efficient phenol-degrading bacteria strain, identified as Bacillus cereus (GenBank No:MN784421) by 16S rDNA analysis, was used for phenol treatment in the water. Under the optimal degradation conditions (10% inoculation of the strain suspension into beef extract peptone medium; 35 °C; pH 7.0; 1% NaCl), the bacterial strain provided 97.71% degradation rate within 46 h of treatment. For improving the tolerance of the strain to phenol toxicity, water hyacinth (Eichhornia crassipes) was used as a raw material to produce a biochar under different pyrolysis temperatures. It has been shown that treatment of 1400 mg/L phenol by a combination of a bacterial strain and 0.6% (w/v) biochar prepared at 550 °C can achieve a degradation rate of 99.5% within 60 h of treatment. It has also been shown that inoculated biochar, embedded and immobilized by calcium alginate to form calcium alginate/biochar microbial microsphere (CABMM with 3-4 mm diameter), could greatly improve the tolerance of bacteria to phenol toxicity. At the initial phenol concentration of 1400 mg/L, using of CABMM enables to achieve 99.1% of the phenol degradation over 40 h of treatment.http://www.sciencedirect.com/science/article/pii/S266701002200155XWater treatmentPhenolBiocharCalcium alginateBacillus cereus |
| spellingShingle | Jian Li Yinjuan Jia Jiaochan Zhong Qinghui Liu Han Li Igor Agranovski Use of calcium alginate/biochar microsphere immobilized bacteria Bacillus sp. for removal of phenol in water Environmental Challenges Water treatment Phenol Biochar Calcium alginate Bacillus cereus |
| title | Use of calcium alginate/biochar microsphere immobilized bacteria Bacillus sp. for removal of phenol in water |
| title_full | Use of calcium alginate/biochar microsphere immobilized bacteria Bacillus sp. for removal of phenol in water |
| title_fullStr | Use of calcium alginate/biochar microsphere immobilized bacteria Bacillus sp. for removal of phenol in water |
| title_full_unstemmed | Use of calcium alginate/biochar microsphere immobilized bacteria Bacillus sp. for removal of phenol in water |
| title_short | Use of calcium alginate/biochar microsphere immobilized bacteria Bacillus sp. for removal of phenol in water |
| title_sort | use of calcium alginate biochar microsphere immobilized bacteria bacillus sp for removal of phenol in water |
| topic | Water treatment Phenol Biochar Calcium alginate Bacillus cereus |
| url | http://www.sciencedirect.com/science/article/pii/S266701002200155X |
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