Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi
Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2000-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100016&tlng=en |
| _version_ | 1797715724776505344 |
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| author | Rosalia Agusti Alicia S Couto Maria Júlia M Alves Walter Colli Rosa M de Lederkremer |
| author_facet | Rosalia Agusti Alicia S Couto Maria Júlia M Alves Walter Colli Rosa M de Lederkremer |
| author_sort | Rosalia Agusti |
| collection | DOAJ |
| description | Trypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites. |
| first_indexed | 2024-03-12T08:10:55Z |
| format | Article |
| id | doaj.art-98787dcd87294518bfec7abd55f615ae |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:10:55Z |
| publishDate | 2000-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-98787dcd87294518bfec7abd55f615ae2023-09-02T19:07:01ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602000-01-019519710210.1590/S0074-02762000000100016Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruziRosalia Agusti0Alicia S Couto1Maria Júlia M Alves2Walter Colli3Rosa M de Lederkremer4Universidad de Buenos AiresUniversidad de Buenos AiresUniversidade de São PauloUniversidade de São PauloUniversidad de Buenos AiresTrypomastigote forms of Trypanosoma cruzi were metabolically labeled with [14C]-ethanolamine and [3H]-palmitic acid. Lipids shed to the culture medium were analyzed and compared with the parasite components. Phosphatidylcholine and lysophosphatidylcholine accounted for 53% of the total incorporated precursor. Interestingly, phosphatidylethanolamine and its lyso derivative lysophosphatidylethanolamine, although present in significant amounts in the parasites, could not be detected in the shed material. Shed lipids were highly enriched in the desaturated fatty acids C16:1 and C18:1 when compared to the total fatty acid pool isolated from the parasites.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100016&tlng=en[14C]-ethanolamine and [3H]-palmitic acid incorporationphospholipidsdesaturated fatty acids |
| spellingShingle | Rosalia Agusti Alicia S Couto Maria Júlia M Alves Walter Colli Rosa M de Lederkremer Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi Memorias do Instituto Oswaldo Cruz [14C]-ethanolamine and [3H]-palmitic acid incorporation phospholipids desaturated fatty acids |
| title | Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi |
| title_full | Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi |
| title_fullStr | Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi |
| title_full_unstemmed | Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi |
| title_short | Lipids shed into the culture medium by trypomastigotes of Trypanosoma cruzi |
| title_sort | lipids shed into the culture medium by trypomastigotes of trypanosoma cruzi |
| topic | [14C]-ethanolamine and [3H]-palmitic acid incorporation phospholipids desaturated fatty acids |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100016&tlng=en |
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