Isolation of four carbapenem-resistant gram-negative species from a single fly
Abstract The widespread occurrence of carbapenem-resistant organisms has garnered significant public attention. Arthropods, including flies, are important vectors of multidrug-resistant bacteria. In this study, we reported the simultaneous carriage of four carbapenem-resistant isolates from differen...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Animal Diseases |
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| Online Access: | https://doi.org/10.1186/s44149-024-00110-5 |
| _version_ | 1797350075228225536 |
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| author | Hanyu Wang Hongwei Zhou Gongxiang Chen Ning Dong |
| author_facet | Hanyu Wang Hongwei Zhou Gongxiang Chen Ning Dong |
| author_sort | Hanyu Wang |
| collection | DOAJ |
| description | Abstract The widespread occurrence of carbapenem-resistant organisms has garnered significant public attention. Arthropods, including flies, are important vectors of multidrug-resistant bacteria. In this study, we reported the simultaneous carriage of four carbapenem-resistant isolates from different species, namely, Escherichia coli (E. coli), Providencia manganoxydans (P. manganoxydan), Myroides odoratimimus (M. odoratimimus) and Proteus mirabilis (P. mirabilis), from a single fly in China. These isolates were characterized through antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing, and bioinformatics analysis. M. odoratimimus showed intrinsic resistance to carbapenems. The mechanisms of carbapenem resistance in E. coli, P. manganoxydans, and P. mirabilis were due to the production of NDM-5, NDM-1 and NDM-1, respectively. Genetic context of the bla NDM genes in these three isolates varied. The bla NDM-5 gene in E. coli was located on an IncHI2/HI2A multidrug-resistant plasmid, which was conjugatively transferable. The bla NDM-1 gene in P. mirabilis resided on the pPM14-NDM_123k-like nonconjugative plasmid. The bla NDM-1 gene in P. manganoxydans was found in a nonconjugatively transferable, multidrug-resistant region. The results of this study enhance our understanding of the dissemination of carbapenem-resistant organisms and suggest the need for a more comprehensive approach to antibiotic resistance research encompassing humans, animals, and the environment. |
| first_indexed | 2024-03-08T12:39:35Z |
| format | Article |
| id | doaj.art-987b81705d2a45caba4f17aeb2ce42b6 |
| institution | Directory Open Access Journal |
| issn | 2731-0442 |
| language | English |
| last_indexed | 2024-03-08T12:39:35Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Animal Diseases |
| spelling | doaj.art-987b81705d2a45caba4f17aeb2ce42b62024-01-21T12:15:50ZengBMCAnimal Diseases2731-04422024-01-01411510.1186/s44149-024-00110-5Isolation of four carbapenem-resistant gram-negative species from a single flyHanyu Wang0Hongwei Zhou1Gongxiang Chen2Ning Dong3Department of Clinical Laboratory Medicine, Second Affiliated Hospital of Zhejiang UniversityDepartment of Clinical Laboratory Medicine, Second Affiliated Hospital of Zhejiang UniversityDepartment of Clinical Laboratory Medicine, Second Affiliated Hospital of Zhejiang UniversityDepartment of Clinical Laboratory Medicine, Second Affiliated Hospital of Zhejiang UniversityAbstract The widespread occurrence of carbapenem-resistant organisms has garnered significant public attention. Arthropods, including flies, are important vectors of multidrug-resistant bacteria. In this study, we reported the simultaneous carriage of four carbapenem-resistant isolates from different species, namely, Escherichia coli (E. coli), Providencia manganoxydans (P. manganoxydan), Myroides odoratimimus (M. odoratimimus) and Proteus mirabilis (P. mirabilis), from a single fly in China. These isolates were characterized through antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing, and bioinformatics analysis. M. odoratimimus showed intrinsic resistance to carbapenems. The mechanisms of carbapenem resistance in E. coli, P. manganoxydans, and P. mirabilis were due to the production of NDM-5, NDM-1 and NDM-1, respectively. Genetic context of the bla NDM genes in these three isolates varied. The bla NDM-5 gene in E. coli was located on an IncHI2/HI2A multidrug-resistant plasmid, which was conjugatively transferable. The bla NDM-1 gene in P. mirabilis resided on the pPM14-NDM_123k-like nonconjugative plasmid. The bla NDM-1 gene in P. manganoxydans was found in a nonconjugatively transferable, multidrug-resistant region. The results of this study enhance our understanding of the dissemination of carbapenem-resistant organisms and suggest the need for a more comprehensive approach to antibiotic resistance research encompassing humans, animals, and the environment.https://doi.org/10.1186/s44149-024-00110-5FlyCarbapenem-resistant organismWhole-genome sequencingPhenotypic characterizationbla NDM |
| spellingShingle | Hanyu Wang Hongwei Zhou Gongxiang Chen Ning Dong Isolation of four carbapenem-resistant gram-negative species from a single fly Animal Diseases Fly Carbapenem-resistant organism Whole-genome sequencing Phenotypic characterization bla NDM |
| title | Isolation of four carbapenem-resistant gram-negative species from a single fly |
| title_full | Isolation of four carbapenem-resistant gram-negative species from a single fly |
| title_fullStr | Isolation of four carbapenem-resistant gram-negative species from a single fly |
| title_full_unstemmed | Isolation of four carbapenem-resistant gram-negative species from a single fly |
| title_short | Isolation of four carbapenem-resistant gram-negative species from a single fly |
| title_sort | isolation of four carbapenem resistant gram negative species from a single fly |
| topic | Fly Carbapenem-resistant organism Whole-genome sequencing Phenotypic characterization bla NDM |
| url | https://doi.org/10.1186/s44149-024-00110-5 |
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