Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica
Abstract Background Many studies have been carried out on the growth-modulating efficiency of plants by the colonization of an endophytic fungus Piriformospora indica. However, studies involving the co-culture of alga with endophytic fungal strains for enhanced biodiesel production are rare. In this...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-019-1516-6 |
| _version_ | 1818264699148435456 |
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| author | Vipul Swarup Bhatnagar Prasun Bandyopadhyay Girish H. Rajacharya Sharanya Sarkar Krishna Mohan Poluri Shashi Kumar |
| author_facet | Vipul Swarup Bhatnagar Prasun Bandyopadhyay Girish H. Rajacharya Sharanya Sarkar Krishna Mohan Poluri Shashi Kumar |
| author_sort | Vipul Swarup Bhatnagar |
| collection | DOAJ |
| description | Abstract Background Many studies have been carried out on the growth-modulating efficiency of plants by the colonization of an endophytic fungus Piriformospora indica. However, studies involving the co-culture of alga with endophytic fungal strains for enhanced biodiesel production are rare. In this study, the interaction between P. indica and Parachlorella kessleri-I, a marine algal strain, was assessed at metabolic level. Results In association with an endophytic fungus, the algal biomass enhanced from 471.6 to 704 mg/L, and the fatty acid methyl ester (FAME) profile of P. kessleri-I increased substantially. In case of FAME profile of co-cultured P. kessleri-I, two essential components of biodiesel, i.e. elaidic acid and oleic acid, increased by 1.4- and 1.8-fold, respectively. To ascertain changes in the metabolic profile of P. kessleri-I by P. indica co-culture, gas chromatography–mass spectrometry (GC–MS)-based untargeted metabolomics study was performed to identify the metabolites involved; and differential nature of the essential metabolites was also confirmed using HPLC and LC–MS. Significant modulation of the bioactive metabolites such as succinate, oxo-propanoate, l-alanine, glutamate, acetate and 1,2 propanediol, hydroxy butane was observed. Conclusion The metabolites like glutamate and succinate that usually belong to the GABA shunt pathway were observed to be upregulated. The pathway links nitrogen metabolism and carbon metabolism, thus influencing the growth and lipid profile of the algae. These differential metabolites thus indicated the important commensal association between the endophytic fungus and autotrophic marine alga, and established that endophytic fungus can be handy for the sustainability of algal biofuel industries. |
| first_indexed | 2024-12-12T19:39:03Z |
| format | Article |
| id | doaj.art-987be4d095c442fea32371992b48efa5 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T19:39:03Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-987be4d095c442fea32371992b48efa52022-12-22T00:14:14ZengBMCBiotechnology for Biofuels1754-68342019-07-0112111910.1186/s13068-019-1516-6Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indicaVipul Swarup Bhatnagar0Prasun Bandyopadhyay1Girish H. Rajacharya2Sharanya Sarkar3Krishna Mohan Poluri4Shashi Kumar5International Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyInternational Centre for Genetic Engineering and BiotechnologyDepartment of Biotechnology & Centre for Transportation Systems (CTRANS), Indian Institute of Technology RoorkeeDepartment of Biotechnology & Centre for Transportation Systems (CTRANS), Indian Institute of Technology RoorkeeInternational Centre for Genetic Engineering and BiotechnologyAbstract Background Many studies have been carried out on the growth-modulating efficiency of plants by the colonization of an endophytic fungus Piriformospora indica. However, studies involving the co-culture of alga with endophytic fungal strains for enhanced biodiesel production are rare. In this study, the interaction between P. indica and Parachlorella kessleri-I, a marine algal strain, was assessed at metabolic level. Results In association with an endophytic fungus, the algal biomass enhanced from 471.6 to 704 mg/L, and the fatty acid methyl ester (FAME) profile of P. kessleri-I increased substantially. In case of FAME profile of co-cultured P. kessleri-I, two essential components of biodiesel, i.e. elaidic acid and oleic acid, increased by 1.4- and 1.8-fold, respectively. To ascertain changes in the metabolic profile of P. kessleri-I by P. indica co-culture, gas chromatography–mass spectrometry (GC–MS)-based untargeted metabolomics study was performed to identify the metabolites involved; and differential nature of the essential metabolites was also confirmed using HPLC and LC–MS. Significant modulation of the bioactive metabolites such as succinate, oxo-propanoate, l-alanine, glutamate, acetate and 1,2 propanediol, hydroxy butane was observed. Conclusion The metabolites like glutamate and succinate that usually belong to the GABA shunt pathway were observed to be upregulated. The pathway links nitrogen metabolism and carbon metabolism, thus influencing the growth and lipid profile of the algae. These differential metabolites thus indicated the important commensal association between the endophytic fungus and autotrophic marine alga, and established that endophytic fungus can be handy for the sustainability of algal biofuel industries.http://link.springer.com/article/10.1186/s13068-019-1516-6CommensalismMetabolomicsEndophytic fungusBiofuelCo-culture |
| spellingShingle | Vipul Swarup Bhatnagar Prasun Bandyopadhyay Girish H. Rajacharya Sharanya Sarkar Krishna Mohan Poluri Shashi Kumar Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica Biotechnology for Biofuels Commensalism Metabolomics Endophytic fungus Biofuel Co-culture |
| title | Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica |
| title_full | Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica |
| title_fullStr | Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica |
| title_full_unstemmed | Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica |
| title_short | Amelioration of biomass and lipid in marine alga by an endophytic fungus Piriformospora indica |
| title_sort | amelioration of biomass and lipid in marine alga by an endophytic fungus piriformospora indica |
| topic | Commensalism Metabolomics Endophytic fungus Biofuel Co-culture |
| url | http://link.springer.com/article/10.1186/s13068-019-1516-6 |
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