Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides folium
The lyophilized venom of the spider Larinioides folium (Araneidae) was analyzed by high-performance liquid chromatography, coupled on-line to electrospray ionization mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (HPLC-ESI-MS/MS), as well as UV-DAD analysis. In combination wi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | deu |
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Swiss Chemical Society
2007-04-01
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| Series: | CHIMIA |
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| Online Access: | https://chimia.ch/chimia/article/view/4300 |
| _version_ | 1828125725342826496 |
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| author | Silvan Eichenberger Laurent Bigler Stefan Bienz |
| author_facet | Silvan Eichenberger Laurent Bigler Stefan Bienz |
| author_sort | Silvan Eichenberger |
| collection | DOAJ |
| description |
The lyophilized venom of the spider Larinioides folium (Araneidae) was analyzed by high-performance liquid chromatography, coupled on-line to electrospray ionization mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (HPLC-ESI-MS/MS), as well as UV-DAD analysis. In
combination with amino acid analysis, high-resolution mass spectrometry and on-column H/D-exchange experiments, the structures of 41 new acyl-polyamines contained in the complex venom of the spider L. folium were elucidated. Data interpretation in detail and the efficiency of this combined
set of analytical method is exemplified by the structural elucidation of one of the toxins, namely of LF503a.
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| first_indexed | 2024-04-11T15:24:53Z |
| format | Article |
| id | doaj.art-987c8417f19e407780aacccb1513d63f |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-04-11T15:24:53Z |
| publishDate | 2007-04-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-987c8417f19e407780aacccb1513d63f2022-12-22T04:16:17ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242007-04-0161410.2533/chimia.2007.161Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides foliumSilvan EichenbergerLaurent BiglerStefan Bienz The lyophilized venom of the spider Larinioides folium (Araneidae) was analyzed by high-performance liquid chromatography, coupled on-line to electrospray ionization mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (HPLC-ESI-MS/MS), as well as UV-DAD analysis. In combination with amino acid analysis, high-resolution mass spectrometry and on-column H/D-exchange experiments, the structures of 41 new acyl-polyamines contained in the complex venom of the spider L. folium were elucidated. Data interpretation in detail and the efficiency of this combined set of analytical method is exemplified by the structural elucidation of one of the toxins, namely of LF503a. https://chimia.ch/chimia/article/view/4300Liquid chromatographyPolyamine alkaloidsSpider venomStructure elucidationTandem mass spectrometry |
| spellingShingle | Silvan Eichenberger Laurent Bigler Stefan Bienz Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides folium CHIMIA Liquid chromatography Polyamine alkaloids Spider venom Structure elucidation Tandem mass spectrometry |
| title | Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides folium |
| title_full | Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides folium |
| title_fullStr | Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides folium |
| title_full_unstemmed | Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides folium |
| title_short | Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides folium |
| title_sort | structure elucidation of polyamine toxins in the venom of the spider larinioides folium |
| topic | Liquid chromatography Polyamine alkaloids Spider venom Structure elucidation Tandem mass spectrometry |
| url | https://chimia.ch/chimia/article/view/4300 |
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