Anti-bacterial effects of the essential oil of Teucrium polium L. on human pathogenic bacteria
Background: Pathogenic bacteria are important causes of disease in humans and agricultural products contamination. Side effects of chemical preservatives necessitate research on the use of a special blend of natural oils to prevent the growth of bacteria. The aim of this study was elucidating the an...
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| Format: | Article |
| Language: | English |
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Farname
2013-09-01
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| Series: | Iranian Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://www.ijmm.ir/browse.php?a_code=A-10-1-192&slc_lang=en&sid=1 |
| _version_ | 1818585632933412864 |
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| author | Mohammad Mohammad Hassan Salari Armita Farahmand |
| author_facet | Mohammad Mohammad Hassan Salari Armita Farahmand |
| author_sort | Mohammad Mohammad |
| collection | DOAJ |
| description | Background: Pathogenic bacteria are important causes of disease in humans and agricultural products contamination. Side effects of chemical preservatives necessitate research on the use of a special blend of natural oils to prevent the growth of bacteria. The aim of this study was elucidating the antibacterial effect of the essential oil of Teucrium polium on human pathogenic bacteria in the Kerman province.
Materials and Methods: In order to investigate the chemical composition and antibacterial activity of essential oil of Teucrium polium, the leaves with young branches of this plants which grows naturally in a village in Kerman province at full flowering stage in May 2010 were collected. The samples were cleaned and then dried in the shade. The plants essential oil was prepared by hydro-distillation method. Essential oil was analyzed by capillary gas chromatography (GC) using flame ionization (FID) and capillary gas chromatography coupled mass spectrometry (GC/MS). The antibacterial activity of the essential oil was determined against Gram negative and Gram positive bacteria by disc diffusion method.
Results: The total oil content of Teucrium polium plant was 0.75%. Twenty eight compounds were identified in the essential oil that included 99.75% of the total oil. The major components were α-pinene (12.52%), Linalool (10.63%) and Caryophyllene oxide (9.69%). For study of antimicrobial activity of the oil sample, the essential oil was tested against 9 bacteria by disc diffusion method. The antimicrobial effects of this essential oil was determined against three Gram positive bacteria Staphylococcus areous (PTCC 1431), Staphylococcus epidermidis (PTCC 1436), Streptococcus faecalis (PTCC 1237); as well as six Gram negative bacteria Pseudomonas aeroginosa (PTCC 11430), Shigella flexneri (PTCC 1716), Kellebsiella pneuomonae(PTCC=1053), Salmonella typhi (PTCC=1609), Serratia marcescens (PTCC 1187) and Escherichia coli (PTCC 1533). The antimicrobial effects of this essential oil on the Gram positive bacteria ( Staphylococcus aureus and Staphylococcus epidermidis ) and on all the Gram negative bacteria tested was much higher than those observed by tetracycline.
Conclusions: The results showed the essential oil of Teucrium polium had strong anti-bacterial effects. The relatively high contents of α-pinene and Linalool in the essential oil may be the cause of its potential medicinal effects |
| first_indexed | 2024-12-16T08:40:10Z |
| format | Article |
| id | doaj.art-9881710118374b9a9d6766f081e57861 |
| institution | Directory Open Access Journal |
| issn | 1735-8612 2345-4342 |
| language | English |
| last_indexed | 2024-12-16T08:40:10Z |
| publishDate | 2013-09-01 |
| publisher | Farname |
| record_format | Article |
| series | Iranian Journal of Medical Microbiology |
| spelling | doaj.art-9881710118374b9a9d6766f081e578612022-12-21T22:37:42ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422013-09-017217Anti-bacterial effects of the essential oil of Teucrium polium L. on human pathogenic bacteriaMohammad Mohammad0Hassan Salari1Armita Farahmand2 Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran Department of Ecology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran Background: Pathogenic bacteria are important causes of disease in humans and agricultural products contamination. Side effects of chemical preservatives necessitate research on the use of a special blend of natural oils to prevent the growth of bacteria. The aim of this study was elucidating the antibacterial effect of the essential oil of Teucrium polium on human pathogenic bacteria in the Kerman province. Materials and Methods: In order to investigate the chemical composition and antibacterial activity of essential oil of Teucrium polium, the leaves with young branches of this plants which grows naturally in a village in Kerman province at full flowering stage in May 2010 were collected. The samples were cleaned and then dried in the shade. The plants essential oil was prepared by hydro-distillation method. Essential oil was analyzed by capillary gas chromatography (GC) using flame ionization (FID) and capillary gas chromatography coupled mass spectrometry (GC/MS). The antibacterial activity of the essential oil was determined against Gram negative and Gram positive bacteria by disc diffusion method. Results: The total oil content of Teucrium polium plant was 0.75%. Twenty eight compounds were identified in the essential oil that included 99.75% of the total oil. The major components were α-pinene (12.52%), Linalool (10.63%) and Caryophyllene oxide (9.69%). For study of antimicrobial activity of the oil sample, the essential oil was tested against 9 bacteria by disc diffusion method. The antimicrobial effects of this essential oil was determined against three Gram positive bacteria Staphylococcus areous (PTCC 1431), Staphylococcus epidermidis (PTCC 1436), Streptococcus faecalis (PTCC 1237); as well as six Gram negative bacteria Pseudomonas aeroginosa (PTCC 11430), Shigella flexneri (PTCC 1716), Kellebsiella pneuomonae(PTCC=1053), Salmonella typhi (PTCC=1609), Serratia marcescens (PTCC 1187) and Escherichia coli (PTCC 1533). The antimicrobial effects of this essential oil on the Gram positive bacteria ( Staphylococcus aureus and Staphylococcus epidermidis ) and on all the Gram negative bacteria tested was much higher than those observed by tetracycline. Conclusions: The results showed the essential oil of Teucrium polium had strong anti-bacterial effects. The relatively high contents of α-pinene and Linalool in the essential oil may be the cause of its potential medicinal effectshttp://www.ijmm.ir/browse.php?a_code=A-10-1-192&slc_lang=en&sid=1Teucrium polium L. antibacterial activity essential oil analytical chemistry |
| spellingShingle | Mohammad Mohammad Hassan Salari Armita Farahmand Anti-bacterial effects of the essential oil of Teucrium polium L. on human pathogenic bacteria Iranian Journal of Medical Microbiology Teucrium polium L. antibacterial activity essential oil analytical chemistry |
| title | Anti-bacterial effects of the essential oil of Teucrium polium L. on human pathogenic bacteria |
| title_full | Anti-bacterial effects of the essential oil of Teucrium polium L. on human pathogenic bacteria |
| title_fullStr | Anti-bacterial effects of the essential oil of Teucrium polium L. on human pathogenic bacteria |
| title_full_unstemmed | Anti-bacterial effects of the essential oil of Teucrium polium L. on human pathogenic bacteria |
| title_short | Anti-bacterial effects of the essential oil of Teucrium polium L. on human pathogenic bacteria |
| title_sort | anti bacterial effects of the essential oil of teucrium polium l on human pathogenic bacteria |
| topic | Teucrium polium L. antibacterial activity essential oil analytical chemistry |
| url | http://www.ijmm.ir/browse.php?a_code=A-10-1-192&slc_lang=en&sid=1 |
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