Organic-Solvent-Resistant Polyimide/Hydroxyapatite Mixed Matrix Membranes for Lysozyme Adsorption
This work reports new mixed matrix membranes (MMMs) for the adsorption of enzymes from organic solvents. In this work, polyimide/hydroxyapatite (PI/HAP) MMMs were prepared via phase inversion method and further crosslinked with 3-aminopropyl triethoxysilane (APTES). The chemical and structural stabi...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/1996-1944/16/22/7210 |
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| author | Junfen Sun Hao Pang Long Chen |
| author_facet | Junfen Sun Hao Pang Long Chen |
| author_sort | Junfen Sun |
| collection | DOAJ |
| description | This work reports new mixed matrix membranes (MMMs) for the adsorption of enzymes from organic solvents. In this work, polyimide/hydroxyapatite (PI/HAP) MMMs were prepared via phase inversion method and further crosslinked with 3-aminopropyl triethoxysilane (APTES). The chemical and structural stability of the crosslinked PI/HAP MMMs were improved and applied for lysozyme (LZ) adsorption in organic solvent. PI/HAP MMMs were crosslinked by changing the 3-aminopropyltriethoxysilane (APTES) concentration and crosslinking time. The optimal APTES crosslinking condition for PI/HAP MMMs is 6% of concentration for 8 h. The LZ adsorption performance was studied by changing solvent types. PI/HAP MMMs possessed a high LZ adsorption in organic-solvent-aqueous solutions, and the LZ adsorption capacity reached 34.1 mg/g. The MMMs had a high desorption capacity and recovery ability. The MMMs maintained 60% of their adsorption capacity and 58% of their desorption at the fourth cycle of adsorption and desorption. The MMMs provided a new technology for the purification and separation of enzymes or proteins by MMMs in organic solvents. |
| first_indexed | 2024-03-09T16:39:18Z |
| format | Article |
| id | doaj.art-9884c3a7ae484d2c8cbb149ba292cc3a |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T16:39:18Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-9884c3a7ae484d2c8cbb149ba292cc3a2023-11-24T14:53:49ZengMDPI AGMaterials1996-19442023-11-011622721010.3390/ma16227210Organic-Solvent-Resistant Polyimide/Hydroxyapatite Mixed Matrix Membranes for Lysozyme AdsorptionJunfen Sun0Hao Pang1Long Chen2State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, North People Road 2999, Shanghai 201620, ChinaState Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, North People Road 2999, Shanghai 201620, ChinaState Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, North People Road 2999, Shanghai 201620, ChinaThis work reports new mixed matrix membranes (MMMs) for the adsorption of enzymes from organic solvents. In this work, polyimide/hydroxyapatite (PI/HAP) MMMs were prepared via phase inversion method and further crosslinked with 3-aminopropyl triethoxysilane (APTES). The chemical and structural stability of the crosslinked PI/HAP MMMs were improved and applied for lysozyme (LZ) adsorption in organic solvent. PI/HAP MMMs were crosslinked by changing the 3-aminopropyltriethoxysilane (APTES) concentration and crosslinking time. The optimal APTES crosslinking condition for PI/HAP MMMs is 6% of concentration for 8 h. The LZ adsorption performance was studied by changing solvent types. PI/HAP MMMs possessed a high LZ adsorption in organic-solvent-aqueous solutions, and the LZ adsorption capacity reached 34.1 mg/g. The MMMs had a high desorption capacity and recovery ability. The MMMs maintained 60% of their adsorption capacity and 58% of their desorption at the fourth cycle of adsorption and desorption. The MMMs provided a new technology for the purification and separation of enzymes or proteins by MMMs in organic solvents.https://www.mdpi.com/1996-1944/16/22/7210polyimidehydroxyapatitemixed matrix membranes (MMMs)crosslinkinglysozyme (LZ) adsorption |
| spellingShingle | Junfen Sun Hao Pang Long Chen Organic-Solvent-Resistant Polyimide/Hydroxyapatite Mixed Matrix Membranes for Lysozyme Adsorption Materials polyimide hydroxyapatite mixed matrix membranes (MMMs) crosslinking lysozyme (LZ) adsorption |
| title | Organic-Solvent-Resistant Polyimide/Hydroxyapatite Mixed Matrix Membranes for Lysozyme Adsorption |
| title_full | Organic-Solvent-Resistant Polyimide/Hydroxyapatite Mixed Matrix Membranes for Lysozyme Adsorption |
| title_fullStr | Organic-Solvent-Resistant Polyimide/Hydroxyapatite Mixed Matrix Membranes for Lysozyme Adsorption |
| title_full_unstemmed | Organic-Solvent-Resistant Polyimide/Hydroxyapatite Mixed Matrix Membranes for Lysozyme Adsorption |
| title_short | Organic-Solvent-Resistant Polyimide/Hydroxyapatite Mixed Matrix Membranes for Lysozyme Adsorption |
| title_sort | organic solvent resistant polyimide hydroxyapatite mixed matrix membranes for lysozyme adsorption |
| topic | polyimide hydroxyapatite mixed matrix membranes (MMMs) crosslinking lysozyme (LZ) adsorption |
| url | https://www.mdpi.com/1996-1944/16/22/7210 |
| work_keys_str_mv | AT junfensun organicsolventresistantpolyimidehydroxyapatitemixedmatrixmembranesforlysozymeadsorption AT haopang organicsolventresistantpolyimidehydroxyapatitemixedmatrixmembranesforlysozymeadsorption AT longchen organicsolventresistantpolyimidehydroxyapatitemixedmatrixmembranesforlysozymeadsorption |