Effect of ZnO-doped magnesium phosphate cements on osteogenic differentiation of mBMSCs in vitro
The insufficient osteogenesis of magnesium phosphate cements (MPCs) limits its further application. It is significant to develop a bioactive MPC with osteogenic properties. In this work, MPCs were reinforced by zinc oxide nanoparticles (ZnO-NPs). The composition, microstructure, setting time, compre...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2022-11-01
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| Series: | Journal of Applied Biomaterials & Functional Materials |
| Online Access: | https://doi.org/10.1177/22808000221136369 |
| _version_ | 1811179989610004480 |
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| author | Xiaomei Wang Feng Shi Dechuan Zhao Yonggang Yan |
| author_facet | Xiaomei Wang Feng Shi Dechuan Zhao Yonggang Yan |
| author_sort | Xiaomei Wang |
| collection | DOAJ |
| description | The insufficient osteogenesis of magnesium phosphate cements (MPCs) limits its further application. It is significant to develop a bioactive MPC with osteogenic properties. In this work, MPCs were reinforced by zinc oxide nanoparticles (ZnO-NPs). The composition, microstructure, setting time, compressive strength and degradation of ZnO-NPs/MPCs (ZNMPCs) were evaluated. The results showed that the setting times of MPCs were prolonged from 8.2 to 25.3 min (5.0ZNMPC). The exothermic temperatures were reduced from 45.8 ± 0.4℃ (MPCs) to 39.3 ± 0.5℃ (1.0ZNMPC). The compressive strength of ZNMPC composite cement with 1 wt. % ZnO-NPs (1.0ZNMPC) was the highest (42.9 MPa) among all the composite cements. Furthermore, the ZNMPCs were cultured with mouse bone marrow mesenchymal stem cells (mBMSCs). The results yielded that the ZNMPCs exhibited good cytocompatibility with enhanced differentiation, proliferation, and mineralization on mBMSCs, and it also pronouncedly elevated the expressions of genes and proteins involving osteogenesis. These findings suggested that ZNMPCs could drive the differentiation toward osteogenesis and mineralization of mBMSCs, providing a simple way to the MPC with enhanced osteogenesis for further orthopedic applications. |
| first_indexed | 2024-04-11T06:43:54Z |
| format | Article |
| id | doaj.art-988740f98b03422f8dae812d455c71b6 |
| institution | Directory Open Access Journal |
| issn | 2280-8000 |
| language | English |
| last_indexed | 2024-04-11T06:43:54Z |
| publishDate | 2022-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Applied Biomaterials & Functional Materials |
| spelling | doaj.art-988740f98b03422f8dae812d455c71b62022-12-22T04:39:25ZengSAGE PublishingJournal of Applied Biomaterials & Functional Materials2280-80002022-11-012010.1177/22808000221136369Effect of ZnO-doped magnesium phosphate cements on osteogenic differentiation of mBMSCs in vitroXiaomei Wang0Feng Shi1Dechuan Zhao2Yonggang Yan3Key Laboratory of Clay Mineral Applied Research of Gansu Province, Center of Eco-Materials and Green Chemistry, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, P. R. ChinaCollaborative Innovation Center of Tissue Repair Material of Sichuan Province, College of Life Sciences, China West Normal University, Nanchong, P. R. ChinaCollaborative Innovation Center of Tissue Repair Material of Sichuan Province, College of Life Sciences, China West Normal University, Nanchong, P. R. ChinaCollege of Physics, Sichuan University, Chengdu, Sichuan, ChinaThe insufficient osteogenesis of magnesium phosphate cements (MPCs) limits its further application. It is significant to develop a bioactive MPC with osteogenic properties. In this work, MPCs were reinforced by zinc oxide nanoparticles (ZnO-NPs). The composition, microstructure, setting time, compressive strength and degradation of ZnO-NPs/MPCs (ZNMPCs) were evaluated. The results showed that the setting times of MPCs were prolonged from 8.2 to 25.3 min (5.0ZNMPC). The exothermic temperatures were reduced from 45.8 ± 0.4℃ (MPCs) to 39.3 ± 0.5℃ (1.0ZNMPC). The compressive strength of ZNMPC composite cement with 1 wt. % ZnO-NPs (1.0ZNMPC) was the highest (42.9 MPa) among all the composite cements. Furthermore, the ZNMPCs were cultured with mouse bone marrow mesenchymal stem cells (mBMSCs). The results yielded that the ZNMPCs exhibited good cytocompatibility with enhanced differentiation, proliferation, and mineralization on mBMSCs, and it also pronouncedly elevated the expressions of genes and proteins involving osteogenesis. These findings suggested that ZNMPCs could drive the differentiation toward osteogenesis and mineralization of mBMSCs, providing a simple way to the MPC with enhanced osteogenesis for further orthopedic applications.https://doi.org/10.1177/22808000221136369 |
| spellingShingle | Xiaomei Wang Feng Shi Dechuan Zhao Yonggang Yan Effect of ZnO-doped magnesium phosphate cements on osteogenic differentiation of mBMSCs in vitro Journal of Applied Biomaterials & Functional Materials |
| title | Effect of ZnO-doped magnesium phosphate cements on osteogenic differentiation of mBMSCs in vitro |
| title_full | Effect of ZnO-doped magnesium phosphate cements on osteogenic differentiation of mBMSCs in vitro |
| title_fullStr | Effect of ZnO-doped magnesium phosphate cements on osteogenic differentiation of mBMSCs in vitro |
| title_full_unstemmed | Effect of ZnO-doped magnesium phosphate cements on osteogenic differentiation of mBMSCs in vitro |
| title_short | Effect of ZnO-doped magnesium phosphate cements on osteogenic differentiation of mBMSCs in vitro |
| title_sort | effect of zno doped magnesium phosphate cements on osteogenic differentiation of mbmscs in vitro |
| url | https://doi.org/10.1177/22808000221136369 |
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