Functional State of Rat’s Erythrocytes Under Different Stress Conditions
Background: In our early publications was shown that electrophorhetic motility of erythrocytes (EPME) is a high effective criteria of adaptation response. This correlation is based on parallel development of adaptation syndrome and activation of the main organism regulatory systems, such as sympatoa...
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| Format: | Article |
| Language: | English |
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"Vikol publishing" ST Kolesnichenko V.V.
2016-08-01
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| Series: | Journal of Stress Physiology & Biochemistry |
| Subjects: | |
| Online Access: | http://www.jspb.ru/issues/2016/N3/JSPB_2016_3_05-11.pdf |
| _version_ | 1828474343961657344 |
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| author | A.A. Martusevich A.V. Deryugina A.K. Martusevich |
| author_facet | A.A. Martusevich A.V. Deryugina A.K. Martusevich |
| author_sort | A.A. Martusevich |
| collection | DOAJ |
| description | Background: In our early publications was shown that electrophorhetic motility of erythrocytes (EPME) is a high effective criteria of adaptation response. This correlation is based on parallel development of adaptation syndrome and activation of the main organism regulatory systems, such as sympatoadrenalic and hypotalamo-hypophosial-adrenal ones.
Objective: study of the influence of physical exercises and adrenaline injections on electrophorhetic motility, membrahes phospholipids spectrum and oxidative metabolism of the rats’ erythrocytes.
Methods: Rats were divided into three equal groups. First group of animals was control (n=10; without any manipulations). Rats of second group were subjected to physical load in the form of a sailing duration of 15 minutes with a cargo amounting to 10% of animal body weight (water temperature – 26-280C). Rats of third group were intraperitoneally injected with adrenaline hydrochloride (0.1 mg/kg). Blood sampling was made from the sublingual vein in 15, 30, 60, 120 minutes and 24 hours after exposure. We estimated the dynamics of the electrophorhetic motility of erythrocytes (EPME), the phospholipid spectrum of erythrocytes membranes, the concentration of malonic dialdehyde (MDA) and the state of the glutathione system.
Results and conclusions: The study suggests that red blood cell as a biological system is capable for realization of stress response may develop a special “alarm reaction” after action of the stress agent. This response initiates activation of free radical processes and phospholipids profile in erythrocyte membranes with reducing of its electronegativity. This stage enhances the activity of the antioxidant system, is limiting the development of lipid peroxidation processes, and leads to the development of "adaptation stage" of the cellular system, coupled with the restoration of the electronegativity of the membrane and the mobilization of reserves of low molecular antioxidants, particularly glutathione, as well as "structural antioxidant", due to the content of readily oxidizable lipid (phosphoethanolamine) and lipids that are resistant to oxidation (phosphatidylcholine, sphingomyeline). |
| first_indexed | 2024-12-11T05:59:11Z |
| format | Article |
| id | doaj.art-988e9953de094dbbae00d34cac06ef28 |
| institution | Directory Open Access Journal |
| issn | 1997-0838 1997-0838 |
| language | English |
| last_indexed | 2024-12-11T05:59:11Z |
| publishDate | 2016-08-01 |
| publisher | "Vikol publishing" ST Kolesnichenko V.V. |
| record_format | Article |
| series | Journal of Stress Physiology & Biochemistry |
| spelling | doaj.art-988e9953de094dbbae00d34cac06ef282022-12-22T01:18:34Zeng"Vikol publishing" ST Kolesnichenko V.V.Journal of Stress Physiology & Biochemistry1997-08381997-08382016-08-01123511Functional State of Rat’s Erythrocytes Under Different Stress ConditionsA.A. Martusevich0A.V. Deryugina1A.K. Martusevich2Department of physiology and biochemistry of the human and animals, National Research Nizhny Novgorod State University named after N.I. Lobachevsky, 603000, Nizhny Novgorod, Gagarin st., 23, RussiaDepartment of physiology and biochemistry of the human and animals, National Research Nizhny Novgorod State University named after N.I. Lobachevsky, 603000, Nizhny Novgorod, Gagarin st., 23, RussiaExperimental medicine department, Privolzhsky Federal Medical Research Centre, 603155, Nizhny Novgorod, Verhnevolzhskaya emb., 18, RussiaBackground: In our early publications was shown that electrophorhetic motility of erythrocytes (EPME) is a high effective criteria of adaptation response. This correlation is based on parallel development of adaptation syndrome and activation of the main organism regulatory systems, such as sympatoadrenalic and hypotalamo-hypophosial-adrenal ones. Objective: study of the influence of physical exercises and adrenaline injections on electrophorhetic motility, membrahes phospholipids spectrum and oxidative metabolism of the rats’ erythrocytes. Methods: Rats were divided into three equal groups. First group of animals was control (n=10; without any manipulations). Rats of second group were subjected to physical load in the form of a sailing duration of 15 minutes with a cargo amounting to 10% of animal body weight (water temperature – 26-280C). Rats of third group were intraperitoneally injected with adrenaline hydrochloride (0.1 mg/kg). Blood sampling was made from the sublingual vein in 15, 30, 60, 120 minutes and 24 hours after exposure. We estimated the dynamics of the electrophorhetic motility of erythrocytes (EPME), the phospholipid spectrum of erythrocytes membranes, the concentration of malonic dialdehyde (MDA) and the state of the glutathione system. Results and conclusions: The study suggests that red blood cell as a biological system is capable for realization of stress response may develop a special “alarm reaction” after action of the stress agent. This response initiates activation of free radical processes and phospholipids profile in erythrocyte membranes with reducing of its electronegativity. This stage enhances the activity of the antioxidant system, is limiting the development of lipid peroxidation processes, and leads to the development of "adaptation stage" of the cellular system, coupled with the restoration of the electronegativity of the membrane and the mobilization of reserves of low molecular antioxidants, particularly glutathione, as well as "structural antioxidant", due to the content of readily oxidizable lipid (phosphoethanolamine) and lipids that are resistant to oxidation (phosphatidylcholine, sphingomyeline).http://www.jspb.ru/issues/2016/N3/JSPB_2016_3_05-11.pdfbloodglutathionemalonic dialdehydestress factorsphospholipids |
| spellingShingle | A.A. Martusevich A.V. Deryugina A.K. Martusevich Functional State of Rat’s Erythrocytes Under Different Stress Conditions Journal of Stress Physiology & Biochemistry blood glutathione malonic dialdehyde stress factors phospholipids |
| title | Functional State of Rat’s Erythrocytes Under Different Stress Conditions |
| title_full | Functional State of Rat’s Erythrocytes Under Different Stress Conditions |
| title_fullStr | Functional State of Rat’s Erythrocytes Under Different Stress Conditions |
| title_full_unstemmed | Functional State of Rat’s Erythrocytes Under Different Stress Conditions |
| title_short | Functional State of Rat’s Erythrocytes Under Different Stress Conditions |
| title_sort | functional state of rat s erythrocytes under different stress conditions |
| topic | blood glutathione malonic dialdehyde stress factors phospholipids |
| url | http://www.jspb.ru/issues/2016/N3/JSPB_2016_3_05-11.pdf |
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