Effects of Thermal Stress on the Gut Microbiome of Juvenile Milkfish (<i>Chanos chanos</i>)
Milkfish, an important aquaculture species in Asian countries, are traditionally cultured in outdoor-based systems. There, they experience potentially stressful fluctuations in environmental conditions, such as temperature, eliciting changes in fish physiology. While the importance of the gut microb...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/1/5 |
| _version_ | 1797543861865676800 |
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| author | Christiane Hassenrück Hannes Reinwald Andreas Kunzmann Inken Tiedemann Astrid Gärdes |
| author_facet | Christiane Hassenrück Hannes Reinwald Andreas Kunzmann Inken Tiedemann Astrid Gärdes |
| author_sort | Christiane Hassenrück |
| collection | DOAJ |
| description | Milkfish, an important aquaculture species in Asian countries, are traditionally cultured in outdoor-based systems. There, they experience potentially stressful fluctuations in environmental conditions, such as temperature, eliciting changes in fish physiology. While the importance of the gut microbiome for the welfare and performance of fish has been recognized, little is known about the effects of thermal stress on the gut microbiome of milkfish and its interactions with the host’s metabolism. We investigated the gut microbiome of juvenile milkfish in a thermal stress experiment, comparing control (26 °C) and elevated temperature (33 °C) treatments over three weeks, analyzing physiological biomarkers, gut microbiome composition, and tank water microbial communities using 16S amplicon sequencing. The gut microbiome was distinct from the tank water and dominated by <i>Cetobacterium</i>, <i>Enterovibrio</i>, and <i>Vibrio</i>. We observed a parallel succession in both temperature treatments, with microbial communities at 33 °C differing more strongly from the control after the initial temperature increase and becoming more similar towards the end of the experiment. As proxy for the fish’s energy status, HSI (hepatosomatic index) was correlated with gut microbiome composition. Our study showed that thermal stress induced changes in the milkfish gut microbiome, which may contribute to the host’s habituation to elevated temperatures over time. |
| first_indexed | 2024-03-10T13:52:27Z |
| format | Article |
| id | doaj.art-9890eb7923074fb68fd9e3f6ff27951a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:52:27Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9890eb7923074fb68fd9e3f6ff27951a2023-11-21T02:02:29ZengMDPI AGMicroorganisms2076-26072020-12-0191510.3390/microorganisms9010005Effects of Thermal Stress on the Gut Microbiome of Juvenile Milkfish (<i>Chanos chanos</i>)Christiane Hassenrück0Hannes Reinwald1Andreas Kunzmann2Inken Tiedemann3Astrid Gärdes4Leibniz Centre for Tropical Marine Research (ZMT), Fahrenheitstraße 6, 28359 Bremen, GermanyMax Planck Institute for Marine Microbiology, Celsiusstraße 1, 28359 Bremen, GermanyLeibniz Centre for Tropical Marine Research (ZMT), Fahrenheitstraße 6, 28359 Bremen, GermanyLeibniz Centre for Tropical Marine Research (ZMT), Fahrenheitstraße 6, 28359 Bremen, GermanyLeibniz Centre for Tropical Marine Research (ZMT), Fahrenheitstraße 6, 28359 Bremen, GermanyMilkfish, an important aquaculture species in Asian countries, are traditionally cultured in outdoor-based systems. There, they experience potentially stressful fluctuations in environmental conditions, such as temperature, eliciting changes in fish physiology. While the importance of the gut microbiome for the welfare and performance of fish has been recognized, little is known about the effects of thermal stress on the gut microbiome of milkfish and its interactions with the host’s metabolism. We investigated the gut microbiome of juvenile milkfish in a thermal stress experiment, comparing control (26 °C) and elevated temperature (33 °C) treatments over three weeks, analyzing physiological biomarkers, gut microbiome composition, and tank water microbial communities using 16S amplicon sequencing. The gut microbiome was distinct from the tank water and dominated by <i>Cetobacterium</i>, <i>Enterovibrio</i>, and <i>Vibrio</i>. We observed a parallel succession in both temperature treatments, with microbial communities at 33 °C differing more strongly from the control after the initial temperature increase and becoming more similar towards the end of the experiment. As proxy for the fish’s energy status, HSI (hepatosomatic index) was correlated with gut microbiome composition. Our study showed that thermal stress induced changes in the milkfish gut microbiome, which may contribute to the host’s habituation to elevated temperatures over time.https://www.mdpi.com/2076-2607/9/1/5aquacultureintestinal microbial communitiestemperature stressenergy metabolism16S rRNA gene sequencing |
| spellingShingle | Christiane Hassenrück Hannes Reinwald Andreas Kunzmann Inken Tiedemann Astrid Gärdes Effects of Thermal Stress on the Gut Microbiome of Juvenile Milkfish (<i>Chanos chanos</i>) Microorganisms aquaculture intestinal microbial communities temperature stress energy metabolism 16S rRNA gene sequencing |
| title | Effects of Thermal Stress on the Gut Microbiome of Juvenile Milkfish (<i>Chanos chanos</i>) |
| title_full | Effects of Thermal Stress on the Gut Microbiome of Juvenile Milkfish (<i>Chanos chanos</i>) |
| title_fullStr | Effects of Thermal Stress on the Gut Microbiome of Juvenile Milkfish (<i>Chanos chanos</i>) |
| title_full_unstemmed | Effects of Thermal Stress on the Gut Microbiome of Juvenile Milkfish (<i>Chanos chanos</i>) |
| title_short | Effects of Thermal Stress on the Gut Microbiome of Juvenile Milkfish (<i>Chanos chanos</i>) |
| title_sort | effects of thermal stress on the gut microbiome of juvenile milkfish i chanos chanos i |
| topic | aquaculture intestinal microbial communities temperature stress energy metabolism 16S rRNA gene sequencing |
| url | https://www.mdpi.com/2076-2607/9/1/5 |
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