Fermentative L-Lactic Acid Production Using <i>Bacillus coagulans</i> from Corn Stalk Deconstructed by an Anaerobic Microbial Community
This study investigated the feasibility of producing L-lactic acid (LA) from dry corn stalk (DCS) that was pretreated by ensiling by an anaerobic microbial community consisting of <i>Bacillus coagulans</i>, <i>Lactobacillus fermentum</i>, and <i>Enterococcus durans</...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/7/611 |
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| author | Xu Yang Zhiyuan Shi Tongyu Wang Xiangyu Meng Lili Song Zhiping Zhang Jingnan Zhang Tao Wei |
| author_facet | Xu Yang Zhiyuan Shi Tongyu Wang Xiangyu Meng Lili Song Zhiping Zhang Jingnan Zhang Tao Wei |
| author_sort | Xu Yang |
| collection | DOAJ |
| description | This study investigated the feasibility of producing L-lactic acid (LA) from dry corn stalk (DCS) that was pretreated by ensiling by an anaerobic microbial community consisting of <i>Bacillus coagulans</i>, <i>Lactobacillus fermentum</i>, and <i>Enterococcus durans</i>. After 28 days of ensiling, the LA and acetic acid content in the microsilage was 2.04 ± 0.08% and 0.38 ± 0.01%, respectively, and the pH was 4.47 ± 0.13. <i>Enterococcus and Lactobacillus</i> became the dominant microbiota during the ensiling process. Twenty-eight-day-old microsilage was then subjected to fermentation by <i>B. coagulans</i> to produce LA in a simultaneous saccharification and co-fermentation process. The enzymatic hydrolysis yield reached >96%. The maximal concentration of LA reached 18.54 ± 0.52 g/L with a substrate concentration of 5%, where the yield of LA was 0.31 ± 0.01 g/g DCS and the optical purity of the product LA was >97%. Anaerobic ensiling is viable for the pretreatment of biomass for the production of value-added chemicals. |
| first_indexed | 2024-03-11T01:05:50Z |
| format | Article |
| id | doaj.art-98911d75402f4294ade02c730c939875 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T01:05:50Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-98911d75402f4294ade02c730c9398752023-11-18T19:15:51ZengMDPI AGFermentation2311-56372023-06-019761110.3390/fermentation9070611Fermentative L-Lactic Acid Production Using <i>Bacillus coagulans</i> from Corn Stalk Deconstructed by an Anaerobic Microbial CommunityXu Yang0Zhiyuan Shi1Tongyu Wang2Xiangyu Meng3Lili Song4Zhiping Zhang5Jingnan Zhang6Tao Wei7School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaThis study investigated the feasibility of producing L-lactic acid (LA) from dry corn stalk (DCS) that was pretreated by ensiling by an anaerobic microbial community consisting of <i>Bacillus coagulans</i>, <i>Lactobacillus fermentum</i>, and <i>Enterococcus durans</i>. After 28 days of ensiling, the LA and acetic acid content in the microsilage was 2.04 ± 0.08% and 0.38 ± 0.01%, respectively, and the pH was 4.47 ± 0.13. <i>Enterococcus and Lactobacillus</i> became the dominant microbiota during the ensiling process. Twenty-eight-day-old microsilage was then subjected to fermentation by <i>B. coagulans</i> to produce LA in a simultaneous saccharification and co-fermentation process. The enzymatic hydrolysis yield reached >96%. The maximal concentration of LA reached 18.54 ± 0.52 g/L with a substrate concentration of 5%, where the yield of LA was 0.31 ± 0.01 g/g DCS and the optical purity of the product LA was >97%. Anaerobic ensiling is viable for the pretreatment of biomass for the production of value-added chemicals.https://www.mdpi.com/2311-5637/9/7/611dry corn stalkensilingmicrobial communityhigh-throughput sequencinglactic acid |
| spellingShingle | Xu Yang Zhiyuan Shi Tongyu Wang Xiangyu Meng Lili Song Zhiping Zhang Jingnan Zhang Tao Wei Fermentative L-Lactic Acid Production Using <i>Bacillus coagulans</i> from Corn Stalk Deconstructed by an Anaerobic Microbial Community Fermentation dry corn stalk ensiling microbial community high-throughput sequencing lactic acid |
| title | Fermentative L-Lactic Acid Production Using <i>Bacillus coagulans</i> from Corn Stalk Deconstructed by an Anaerobic Microbial Community |
| title_full | Fermentative L-Lactic Acid Production Using <i>Bacillus coagulans</i> from Corn Stalk Deconstructed by an Anaerobic Microbial Community |
| title_fullStr | Fermentative L-Lactic Acid Production Using <i>Bacillus coagulans</i> from Corn Stalk Deconstructed by an Anaerobic Microbial Community |
| title_full_unstemmed | Fermentative L-Lactic Acid Production Using <i>Bacillus coagulans</i> from Corn Stalk Deconstructed by an Anaerobic Microbial Community |
| title_short | Fermentative L-Lactic Acid Production Using <i>Bacillus coagulans</i> from Corn Stalk Deconstructed by an Anaerobic Microbial Community |
| title_sort | fermentative l lactic acid production using i bacillus coagulans i from corn stalk deconstructed by an anaerobic microbial community |
| topic | dry corn stalk ensiling microbial community high-throughput sequencing lactic acid |
| url | https://www.mdpi.com/2311-5637/9/7/611 |
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