Identification and Validation of <i>Toxoplasma gondii</i> Mitoribosomal Large Subunit Components
Mitochondrial ribosomes are fundamental to mitochondrial function, and thus survival, of nearly all eukaryotes. Despite their common ancestry, mitoribosomes have evolved divergent features in different eukaryotic lineages. In apicomplexans, the mitochondrial rRNA is extremely fragmented raising ques...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/5/863 |
| _version_ | 1797497721022578688 |
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| author | Shikha Shikha Mariana Ferreira Silva Lilach Sheiner |
| author_facet | Shikha Shikha Mariana Ferreira Silva Lilach Sheiner |
| author_sort | Shikha Shikha |
| collection | DOAJ |
| description | Mitochondrial ribosomes are fundamental to mitochondrial function, and thus survival, of nearly all eukaryotes. Despite their common ancestry, mitoribosomes have evolved divergent features in different eukaryotic lineages. In apicomplexans, the mitochondrial rRNA is extremely fragmented raising questions about its evolution, protein composition and structure. Apicomplexan mitochondrial translation and the mitoribosomes are essential in all parasites and life stages studied, highlighting mitoribosomes as a promising target for drugs. Still, the apicomplexan mitoribosome is understudied, with one of the obstacles being that its composition is unknown. Here, to facilitate the study of apicomplexan mitoribosomes, we identified and validated components of the mitoribosomal large subunit in the model apicomplexan <i>Toxoplasma gondii</i>. |
| first_indexed | 2024-03-10T03:23:17Z |
| format | Article |
| id | doaj.art-989467379c36460982b42a771f059da3 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:23:17Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-989467379c36460982b42a771f059da32023-11-23T12:14:06ZengMDPI AGMicroorganisms2076-26072022-04-0110586310.3390/microorganisms10050863Identification and Validation of <i>Toxoplasma gondii</i> Mitoribosomal Large Subunit ComponentsShikha Shikha0Mariana Ferreira Silva1Lilach Sheiner2Wellcome Centre for Integrative Parasitology, University of Glasgow, 120 University Place, Glasgow G12 8TA, UKWellcome Centre for Integrative Parasitology, University of Glasgow, 120 University Place, Glasgow G12 8TA, UKWellcome Centre for Integrative Parasitology, University of Glasgow, 120 University Place, Glasgow G12 8TA, UKMitochondrial ribosomes are fundamental to mitochondrial function, and thus survival, of nearly all eukaryotes. Despite their common ancestry, mitoribosomes have evolved divergent features in different eukaryotic lineages. In apicomplexans, the mitochondrial rRNA is extremely fragmented raising questions about its evolution, protein composition and structure. Apicomplexan mitochondrial translation and the mitoribosomes are essential in all parasites and life stages studied, highlighting mitoribosomes as a promising target for drugs. Still, the apicomplexan mitoribosome is understudied, with one of the obstacles being that its composition is unknown. Here, to facilitate the study of apicomplexan mitoribosomes, we identified and validated components of the mitoribosomal large subunit in the model apicomplexan <i>Toxoplasma gondii</i>.https://www.mdpi.com/2076-2607/10/5/863<i>Toxoplasma</i>mitochondrionribosomemitoribosomeapicomplexaparasite |
| spellingShingle | Shikha Shikha Mariana Ferreira Silva Lilach Sheiner Identification and Validation of <i>Toxoplasma gondii</i> Mitoribosomal Large Subunit Components Microorganisms <i>Toxoplasma</i> mitochondrion ribosome mitoribosome apicomplexa parasite |
| title | Identification and Validation of <i>Toxoplasma gondii</i> Mitoribosomal Large Subunit Components |
| title_full | Identification and Validation of <i>Toxoplasma gondii</i> Mitoribosomal Large Subunit Components |
| title_fullStr | Identification and Validation of <i>Toxoplasma gondii</i> Mitoribosomal Large Subunit Components |
| title_full_unstemmed | Identification and Validation of <i>Toxoplasma gondii</i> Mitoribosomal Large Subunit Components |
| title_short | Identification and Validation of <i>Toxoplasma gondii</i> Mitoribosomal Large Subunit Components |
| title_sort | identification and validation of i toxoplasma gondii i mitoribosomal large subunit components |
| topic | <i>Toxoplasma</i> mitochondrion ribosome mitoribosome apicomplexa parasite |
| url | https://www.mdpi.com/2076-2607/10/5/863 |
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