Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of Deoxynivalenol
Immunoassay has the advantages of high sensitivity, high specificity, and simple operation, and has been widely used in the detection of mycotoxins. For several years, time-resolved fluorescence immunochromatography (TRFIA) paper-based sensors have attracted much attention as a simple and low-cost f...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/22/6577 |
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| author | Haowei Dong Xingshuang An Yaodong Xiang Fukai Guan Qi Zhang Qingqing Yang Xia Sun Yemin Guo |
| author_facet | Haowei Dong Xingshuang An Yaodong Xiang Fukai Guan Qi Zhang Qingqing Yang Xia Sun Yemin Guo |
| author_sort | Haowei Dong |
| collection | DOAJ |
| description | Immunoassay has the advantages of high sensitivity, high specificity, and simple operation, and has been widely used in the detection of mycotoxins. For several years, time-resolved fluorescence immunochromatography (TRFIA) paper-based sensors have attracted much attention as a simple and low-cost field detection technology. However, a traditional TRFIA paper-based sensor is based on antibody labeling, which cannot easily meet the current detection requirements. A second antibody labeling method was used to amplify the fluorescence signal and improve the detection sensitivity. Polystyrene fluorescent microspheres were combined with sheep anti-mouse IgG to prepare fluorescent probes (Eu-IgGs). After the probe fully reacted with the antibody (Eu-IgGs-Abs) in the sample cell, it was deployed on the paper-based sensor using chromatography. Eu-IgGs-Abs that were not bound to the target were captured on the T-line, while those that were bound were captured on the C-line. The paper-based sensor reflected the corresponding fluorescence intensity change. Because a single molecule of the deoxynivalenol antibody could bind to multiple Eu-IgGs, this method could amplify the fluorescence signal intensity on the unit antibody and improve the detection sensitivity. The working standard curve of the sensor was established under the optimum working conditions. It showed the lower limit of detection and higher recovery rate when it was applied to actual samples and compared with other methods. This sensor has the advantages of high sensitivity, good accuracy, and good specificity, saving the amount of antibody consumed and being suitable for rapid field detection of deoxynivalenol. |
| first_indexed | 2024-03-10T14:46:57Z |
| format | Article |
| id | doaj.art-9897bd5b676d41669351956533e986c9 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T14:46:57Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-9897bd5b676d41669351956533e986c92023-11-20T21:20:28ZengMDPI AGSensors1424-82202020-11-012022657710.3390/s20226577Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of DeoxynivalenolHaowei Dong0Xingshuang An1Yaodong Xiang2Fukai Guan3Qi Zhang4Qingqing Yang5Xia Sun6Yemin Guo7School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, ChinaOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, ChinaSchool of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun West Road, Zibo 255049, ChinaImmunoassay has the advantages of high sensitivity, high specificity, and simple operation, and has been widely used in the detection of mycotoxins. For several years, time-resolved fluorescence immunochromatography (TRFIA) paper-based sensors have attracted much attention as a simple and low-cost field detection technology. However, a traditional TRFIA paper-based sensor is based on antibody labeling, which cannot easily meet the current detection requirements. A second antibody labeling method was used to amplify the fluorescence signal and improve the detection sensitivity. Polystyrene fluorescent microspheres were combined with sheep anti-mouse IgG to prepare fluorescent probes (Eu-IgGs). After the probe fully reacted with the antibody (Eu-IgGs-Abs) in the sample cell, it was deployed on the paper-based sensor using chromatography. Eu-IgGs-Abs that were not bound to the target were captured on the T-line, while those that were bound were captured on the C-line. The paper-based sensor reflected the corresponding fluorescence intensity change. Because a single molecule of the deoxynivalenol antibody could bind to multiple Eu-IgGs, this method could amplify the fluorescence signal intensity on the unit antibody and improve the detection sensitivity. The working standard curve of the sensor was established under the optimum working conditions. It showed the lower limit of detection and higher recovery rate when it was applied to actual samples and compared with other methods. This sensor has the advantages of high sensitivity, good accuracy, and good specificity, saving the amount of antibody consumed and being suitable for rapid field detection of deoxynivalenol.https://www.mdpi.com/1424-8220/20/22/6577deoxynivalenolsecondary antibody labelingtime-resolved fluorescence immunochromatographyfield detection methodpaper-based sensor |
| spellingShingle | Haowei Dong Xingshuang An Yaodong Xiang Fukai Guan Qi Zhang Qingqing Yang Xia Sun Yemin Guo Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of Deoxynivalenol Sensors deoxynivalenol secondary antibody labeling time-resolved fluorescence immunochromatography field detection method paper-based sensor |
| title | Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of Deoxynivalenol |
| title_full | Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of Deoxynivalenol |
| title_fullStr | Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of Deoxynivalenol |
| title_full_unstemmed | Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of Deoxynivalenol |
| title_short | Novel Time-Resolved Fluorescence Immunochromatography Paper-Based Sensor with Signal Amplification Strategy for Detection of Deoxynivalenol |
| title_sort | novel time resolved fluorescence immunochromatography paper based sensor with signal amplification strategy for detection of deoxynivalenol |
| topic | deoxynivalenol secondary antibody labeling time-resolved fluorescence immunochromatography field detection method paper-based sensor |
| url | https://www.mdpi.com/1424-8220/20/22/6577 |
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