Immunohistochemical and ultrastructural analysis of the effect of omega-3 on embryonic implantation in an experimental mouse model
Objective: Implantation is the first step to a healthy pregnancy. Omega-3 supplementation is common to use during pregnancy, for its antioxidant and membrane stabilising effect. In this study we have aimed to study the effect of Omega-3 supplementation on implantation in a mouse model by immunohisto...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-06-01
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| Series: | Taiwanese Journal of Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S102845591630033X |
| _version_ | 1811316049725882368 |
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| author | Kemal Sarsmaz Asli Goker Serap Cilaker Micili Bekir Ugur Ergur Naci Kemal Kuscu |
| author_facet | Kemal Sarsmaz Asli Goker Serap Cilaker Micili Bekir Ugur Ergur Naci Kemal Kuscu |
| author_sort | Kemal Sarsmaz |
| collection | DOAJ |
| description | Objective: Implantation is the first step to a healthy pregnancy. Omega-3 supplementation is common to use during pregnancy, for its antioxidant and membrane stabilising effect. In this study we have aimed to study the effect of Omega-3 supplementation on implantation in a mouse model by immunohistochemical methods and electron microscopic evaluation.
Materials and methods: Mice were randomized into three groups to receive standard food, Omega-3 400 mg/kg and Omega-3 1000 mg/kg one menstrual cycle before mating. Mice were sacrificed on third day of estimated implantation and uterine horns were evaluated immunohistochemically for staining of Laminin and Leukemia Inhibitory Factor (LIF) and ultrastructural morphology.
Results: Laminin and LIF immunoreactivity were increased signifcantly in the high dose group when compared to the control and low-dose groups in lumen epithelium basal membrane, gland epithelium basal membrane and endometrial stroma. Electron-microscopic evaluation showed a decrease in epithelial height and microvilli loss in the high dose groups.
Conclusion: Omega-3 supplementation increased implantation markers Laminin and LIF and decreased epithelial height and microvilli thus seems to prepare the endometrium for a favorable environment of implantation. |
| first_indexed | 2024-04-13T11:42:04Z |
| format | Article |
| id | doaj.art-989d0213c2fb49ba9e3ac362b055a504 |
| institution | Directory Open Access Journal |
| issn | 1028-4559 |
| language | English |
| last_indexed | 2024-04-13T11:42:04Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Taiwanese Journal of Obstetrics & Gynecology |
| spelling | doaj.art-989d0213c2fb49ba9e3ac362b055a5042022-12-22T02:48:18ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592016-06-0155335135610.1016/j.tjog.2016.04.011Immunohistochemical and ultrastructural analysis of the effect of omega-3 on embryonic implantation in an experimental mouse modelKemal Sarsmaz0Asli Goker1Serap Cilaker Micili2Bekir Ugur Ergur3Naci Kemal Kuscu4Department of Obstetrics and Gynecology, Celal Bayar University Faculty of Medicine, Manisa, TurkeyDepartment of Obstetrics and Gynecology, Celal Bayar University Faculty of Medicine, Manisa, TurkeyDepartment of Histology and Embryology, Dokuz Eylul University Faculty of Medicine, Izmir, TurkeyDepartment of Histology and Embryology, Dokuz Eylul University Faculty of Medicine, Izmir, TurkeyDepartment of Obstetrics and Gynecology, Celal Bayar University Faculty of Medicine, Manisa, TurkeyObjective: Implantation is the first step to a healthy pregnancy. Omega-3 supplementation is common to use during pregnancy, for its antioxidant and membrane stabilising effect. In this study we have aimed to study the effect of Omega-3 supplementation on implantation in a mouse model by immunohistochemical methods and electron microscopic evaluation. Materials and methods: Mice were randomized into three groups to receive standard food, Omega-3 400 mg/kg and Omega-3 1000 mg/kg one menstrual cycle before mating. Mice were sacrificed on third day of estimated implantation and uterine horns were evaluated immunohistochemically for staining of Laminin and Leukemia Inhibitory Factor (LIF) and ultrastructural morphology. Results: Laminin and LIF immunoreactivity were increased signifcantly in the high dose group when compared to the control and low-dose groups in lumen epithelium basal membrane, gland epithelium basal membrane and endometrial stroma. Electron-microscopic evaluation showed a decrease in epithelial height and microvilli loss in the high dose groups. Conclusion: Omega-3 supplementation increased implantation markers Laminin and LIF and decreased epithelial height and microvilli thus seems to prepare the endometrium for a favorable environment of implantation.http://www.sciencedirect.com/science/article/pii/S102845591630033Xelectron microscopeimplantationlamininleukemia inhibitory factoromega-3 |
| spellingShingle | Kemal Sarsmaz Asli Goker Serap Cilaker Micili Bekir Ugur Ergur Naci Kemal Kuscu Immunohistochemical and ultrastructural analysis of the effect of omega-3 on embryonic implantation in an experimental mouse model Taiwanese Journal of Obstetrics & Gynecology electron microscope implantation laminin leukemia inhibitory factor omega-3 |
| title | Immunohistochemical and ultrastructural analysis of the effect of omega-3 on embryonic implantation in an experimental mouse model |
| title_full | Immunohistochemical and ultrastructural analysis of the effect of omega-3 on embryonic implantation in an experimental mouse model |
| title_fullStr | Immunohistochemical and ultrastructural analysis of the effect of omega-3 on embryonic implantation in an experimental mouse model |
| title_full_unstemmed | Immunohistochemical and ultrastructural analysis of the effect of omega-3 on embryonic implantation in an experimental mouse model |
| title_short | Immunohistochemical and ultrastructural analysis of the effect of omega-3 on embryonic implantation in an experimental mouse model |
| title_sort | immunohistochemical and ultrastructural analysis of the effect of omega 3 on embryonic implantation in an experimental mouse model |
| topic | electron microscope implantation laminin leukemia inhibitory factor omega-3 |
| url | http://www.sciencedirect.com/science/article/pii/S102845591630033X |
| work_keys_str_mv | AT kemalsarsmaz immunohistochemicalandultrastructuralanalysisoftheeffectofomega3onembryonicimplantationinanexperimentalmousemodel AT asligoker immunohistochemicalandultrastructuralanalysisoftheeffectofomega3onembryonicimplantationinanexperimentalmousemodel AT serapcilakermicili immunohistochemicalandultrastructuralanalysisoftheeffectofomega3onembryonicimplantationinanexperimentalmousemodel AT bekirugurergur immunohistochemicalandultrastructuralanalysisoftheeffectofomega3onembryonicimplantationinanexperimentalmousemodel AT nacikemalkuscu immunohistochemicalandultrastructuralanalysisoftheeffectofomega3onembryonicimplantationinanexperimentalmousemodel |