Combination of Meropenem and Zinc Oxide Nanoparticles; Antimicrobial Synergism, Exaggerated Antibiofilm Activity, and Efficient Therapeutic Strategy against Bacterial Keratitis
<i>Pseudomonas aeruginosa</i> is an opportunistic gram-negative human pathogen that causes a wide range of infections, including nosocomial infections. Aside from the intrinsic and acquired antimicrobial resistance against many classes of antibiotics, <i>P. aeruginosa</i> can...
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MDPI AG
2022-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/10/1374 |
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| author | Mohamed El-Telbany Alzhraa Ali Mohamed Galal Yahya Aliaa Abdelghafar Mahmoud Saad Abdel-Halim Sameh Saber Mohamed A. Alfaleh Asmaa H. Mohamed Fatma Abdelrahman Hoda A. Fathey Gehad H. Ali Mohamed Abdel-Haleem |
| author_facet | Mohamed El-Telbany Alzhraa Ali Mohamed Galal Yahya Aliaa Abdelghafar Mahmoud Saad Abdel-Halim Sameh Saber Mohamed A. Alfaleh Asmaa H. Mohamed Fatma Abdelrahman Hoda A. Fathey Gehad H. Ali Mohamed Abdel-Haleem |
| author_sort | Mohamed El-Telbany |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> is an opportunistic gram-negative human pathogen that causes a wide range of infections, including nosocomial infections. Aside from the intrinsic and acquired antimicrobial resistance against many classes of antibiotics, <i>P. aeruginosa</i> can produce an extracellular polymeric matrix called “biofilm” that protects bacteria from antibiotics and harmful factors. Biofilm enables <i>P. aeruginosa</i> to develop chronic infections. This study assessed the inhibitory action of ZnO-nanoparticles against biofilms formed by multidrug-resistant <i>P. aeruginosa</i> strains. A collection of 24 clinical strains of <i>P. aeruginosa</i> were tested for their antimicrobial resistance against different antibiotics using the disk diffusion method. The antibiofilm activity of ZnO-NPs was assessed using the microtiter plate biofilm assay. The application of ZnO-NPs dramatically modulated the resistance profile and biofilm activity of <i>P. aeruginosa</i>. The combination of ZnO-NPs and meropenem showed synergistic antipseudomonal activity with lower MICs. The scanning electron microscope (SEM) micrographs revealed complete inhibition of biofilms treated with the meropenem–ZnO-NPs combination. Reduced expression of biofilm regulating genes <i>lasR</i>, <i>pslA,</i> and <i>fliC</i> was detected, reflecting the enhanced antibiofilm effect of ZnO-NPs. In vivo application of this antimicrobial mixture completely cured <i>P. aeruginosa</i>-induced keratitis in rats. Our findings represent a dual enhancement of antibacterial and antibiofilm activity via the use of meropenem–ZnO-NPs combination against carbapenem-resistant <i>P. aeruginosa</i> infections. |
| first_indexed | 2024-03-09T20:50:49Z |
| format | Article |
| id | doaj.art-989d18b2a77740e0903439cc5e295505 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T20:50:49Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-989d18b2a77740e0903439cc5e2955052023-11-23T22:35:23ZengMDPI AGAntibiotics2079-63822022-10-011110137410.3390/antibiotics11101374Combination of Meropenem and Zinc Oxide Nanoparticles; Antimicrobial Synergism, Exaggerated Antibiofilm Activity, and Efficient Therapeutic Strategy against Bacterial KeratitisMohamed El-Telbany0Alzhraa Ali Mohamed1Galal Yahya2Aliaa Abdelghafar3Mahmoud Saad Abdel-Halim4Sameh Saber5Mohamed A. Alfaleh6Asmaa H. Mohamed7Fatma Abdelrahman8Hoda A. Fathey9Gehad H. Ali10Mohamed Abdel-Haleem11Microbiology and Botany Department, Faculty of Science, Zagazig University, Zagazig 44519, EgyptMicrobiology and Botany Department, Faculty of Science, Zagazig University, Zagazig 44519, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, EgyptDepartment of Pharmacology, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa 11152, EgyptDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaMicrobiology and Botany Department, Faculty of Science, Zagazig University, Zagazig 44519, EgyptCenter for Microbiology and Phage Therapy, Zewail City of Science and Technology, Giza 12578, EgyptMicrobiology and Botany Department, Faculty of Science, Zagazig University, Zagazig 44519, EgyptMicrobiology and Botany Department, Faculty of Science, Zagazig University, Zagazig 44519, EgyptMicrobiology and Botany Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt<i>Pseudomonas aeruginosa</i> is an opportunistic gram-negative human pathogen that causes a wide range of infections, including nosocomial infections. Aside from the intrinsic and acquired antimicrobial resistance against many classes of antibiotics, <i>P. aeruginosa</i> can produce an extracellular polymeric matrix called “biofilm” that protects bacteria from antibiotics and harmful factors. Biofilm enables <i>P. aeruginosa</i> to develop chronic infections. This study assessed the inhibitory action of ZnO-nanoparticles against biofilms formed by multidrug-resistant <i>P. aeruginosa</i> strains. A collection of 24 clinical strains of <i>P. aeruginosa</i> were tested for their antimicrobial resistance against different antibiotics using the disk diffusion method. The antibiofilm activity of ZnO-NPs was assessed using the microtiter plate biofilm assay. The application of ZnO-NPs dramatically modulated the resistance profile and biofilm activity of <i>P. aeruginosa</i>. The combination of ZnO-NPs and meropenem showed synergistic antipseudomonal activity with lower MICs. The scanning electron microscope (SEM) micrographs revealed complete inhibition of biofilms treated with the meropenem–ZnO-NPs combination. Reduced expression of biofilm regulating genes <i>lasR</i>, <i>pslA,</i> and <i>fliC</i> was detected, reflecting the enhanced antibiofilm effect of ZnO-NPs. In vivo application of this antimicrobial mixture completely cured <i>P. aeruginosa</i>-induced keratitis in rats. Our findings represent a dual enhancement of antibacterial and antibiofilm activity via the use of meropenem–ZnO-NPs combination against carbapenem-resistant <i>P. aeruginosa</i> infections.https://www.mdpi.com/2079-6382/11/10/1374<i>Pseudomonas aeruginosa</i>biofilmsantimicrobial resistanceZnO-NPsgene expressionmeropenem |
| spellingShingle | Mohamed El-Telbany Alzhraa Ali Mohamed Galal Yahya Aliaa Abdelghafar Mahmoud Saad Abdel-Halim Sameh Saber Mohamed A. Alfaleh Asmaa H. Mohamed Fatma Abdelrahman Hoda A. Fathey Gehad H. Ali Mohamed Abdel-Haleem Combination of Meropenem and Zinc Oxide Nanoparticles; Antimicrobial Synergism, Exaggerated Antibiofilm Activity, and Efficient Therapeutic Strategy against Bacterial Keratitis Antibiotics <i>Pseudomonas aeruginosa</i> biofilms antimicrobial resistance ZnO-NPs gene expression meropenem |
| title | Combination of Meropenem and Zinc Oxide Nanoparticles; Antimicrobial Synergism, Exaggerated Antibiofilm Activity, and Efficient Therapeutic Strategy against Bacterial Keratitis |
| title_full | Combination of Meropenem and Zinc Oxide Nanoparticles; Antimicrobial Synergism, Exaggerated Antibiofilm Activity, and Efficient Therapeutic Strategy against Bacterial Keratitis |
| title_fullStr | Combination of Meropenem and Zinc Oxide Nanoparticles; Antimicrobial Synergism, Exaggerated Antibiofilm Activity, and Efficient Therapeutic Strategy against Bacterial Keratitis |
| title_full_unstemmed | Combination of Meropenem and Zinc Oxide Nanoparticles; Antimicrobial Synergism, Exaggerated Antibiofilm Activity, and Efficient Therapeutic Strategy against Bacterial Keratitis |
| title_short | Combination of Meropenem and Zinc Oxide Nanoparticles; Antimicrobial Synergism, Exaggerated Antibiofilm Activity, and Efficient Therapeutic Strategy against Bacterial Keratitis |
| title_sort | combination of meropenem and zinc oxide nanoparticles antimicrobial synergism exaggerated antibiofilm activity and efficient therapeutic strategy against bacterial keratitis |
| topic | <i>Pseudomonas aeruginosa</i> biofilms antimicrobial resistance ZnO-NPs gene expression meropenem |
| url | https://www.mdpi.com/2079-6382/11/10/1374 |
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