Comparison of Antibacterial Activities of ProRoot MTA, OrthoMTA, and RetroMTA Against Three Anaerobic Endodontic Bacteria
Objectives: The aim of this study was to assess the antibacterial activities of OrthoMTA, RetroMTA, and ProRoot MTA against Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), and Prevotella intermedia (Pi). Materials and Methods: Each material was mixed on a glass slab using a spatula and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2018-10-01
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| Series: | Frontiers in Dentistry |
| Subjects: | |
| Online Access: | https://jdt.tums.ac.ir/index.php/jdt/article/view/2404 |
| _version_ | 1811295958305079296 |
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| author | Sedigheh Khedmat Mohammad Aminipor Maryam Pourhajibagher Mohammad Javad Kharazifar Abbas Bahador |
| author_facet | Sedigheh Khedmat Mohammad Aminipor Maryam Pourhajibagher Mohammad Javad Kharazifar Abbas Bahador |
| author_sort | Sedigheh Khedmat |
| collection | DOAJ |
| description | Objectives: The aim of this study was to assess the antibacterial activities of OrthoMTA, RetroMTA, and ProRoot MTA against Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), and Prevotella intermedia (Pi).
Materials and Methods: Each material was mixed on a glass slab using a spatula and was placed in columns containing the filter membrane of the modified membrane-enclosed immersion test (MEIT) system. The materials were sterilized after setting. The columns containing the sterilized test materials were placed in microcentrifuge tubes containing 500 µl of bacterial suspension. The systems were then incubated at 37°C under anaerobic conditions. After 72 hours, the bacterial growth and concentration (colony-forming unit (CFU)/ml) were assessed. The results were analyzed using one-way analysis of variance (ANOVA) and Tukey's post-hoc test in SPSS 22 software. In all analyses, the differences were considered significant at P<0.05.
Results: OrthoMTA had the highest antibacterial activity against Pi. The mean number of CFU/ml of Fn in the presence of ProRoot MTA and RetroMTA was significantly lower than that in positive controls. There were significant differences between the antibacterial activities of ProRoot MTA and OrthoMTA against Pg compared to positive controls.
Conclusions: ProRoot MTA, OrthoMTA, and RetroMTA had similar antibacterial activities against the three evaluated anaerobic endodontic bacteria, except RetroMTA against Pg. |
| first_indexed | 2024-04-13T05:41:17Z |
| format | Article |
| id | doaj.art-989d39c718d9447e931de6f8c643f259 |
| institution | Directory Open Access Journal |
| issn | 2676-296X |
| language | English |
| last_indexed | 2024-04-13T05:41:17Z |
| publishDate | 2018-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Frontiers in Dentistry |
| spelling | doaj.art-989d39c718d9447e931de6f8c643f2592022-12-22T03:00:05ZengTehran University of Medical SciencesFrontiers in Dentistry2676-296X2018-10-011552404Comparison of Antibacterial Activities of ProRoot MTA, OrthoMTA, and RetroMTA Against Three Anaerobic Endodontic BacteriaSedigheh Khedmat0Mohammad Aminipor1Maryam Pourhajibagher2Mohammad Javad Kharazifar3Abbas Bahador4Professor, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranDentist, Dental School, Tehran University of Medical Sciences, Tehran, IranResearcher, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, IranStatistical Advisor, Dental Research Center, Tehran University of Medical Sciences, Tehran, IranAssociate Professor, Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranObjectives: The aim of this study was to assess the antibacterial activities of OrthoMTA, RetroMTA, and ProRoot MTA against Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), and Prevotella intermedia (Pi). Materials and Methods: Each material was mixed on a glass slab using a spatula and was placed in columns containing the filter membrane of the modified membrane-enclosed immersion test (MEIT) system. The materials were sterilized after setting. The columns containing the sterilized test materials were placed in microcentrifuge tubes containing 500 µl of bacterial suspension. The systems were then incubated at 37°C under anaerobic conditions. After 72 hours, the bacterial growth and concentration (colony-forming unit (CFU)/ml) were assessed. The results were analyzed using one-way analysis of variance (ANOVA) and Tukey's post-hoc test in SPSS 22 software. In all analyses, the differences were considered significant at P<0.05. Results: OrthoMTA had the highest antibacterial activity against Pi. The mean number of CFU/ml of Fn in the presence of ProRoot MTA and RetroMTA was significantly lower than that in positive controls. There were significant differences between the antibacterial activities of ProRoot MTA and OrthoMTA against Pg compared to positive controls. Conclusions: ProRoot MTA, OrthoMTA, and RetroMTA had similar antibacterial activities against the three evaluated anaerobic endodontic bacteria, except RetroMTA against Pg.https://jdt.tums.ac.ir/index.php/jdt/article/view/2404Bacterial Sensitivity TestMineral Trioxide AggregateOrthoMTAProRoot MTARetroMTA |
| spellingShingle | Sedigheh Khedmat Mohammad Aminipor Maryam Pourhajibagher Mohammad Javad Kharazifar Abbas Bahador Comparison of Antibacterial Activities of ProRoot MTA, OrthoMTA, and RetroMTA Against Three Anaerobic Endodontic Bacteria Frontiers in Dentistry Bacterial Sensitivity Test Mineral Trioxide Aggregate OrthoMTA ProRoot MTA RetroMTA |
| title | Comparison of Antibacterial Activities of ProRoot MTA, OrthoMTA, and RetroMTA Against Three Anaerobic Endodontic Bacteria |
| title_full | Comparison of Antibacterial Activities of ProRoot MTA, OrthoMTA, and RetroMTA Against Three Anaerobic Endodontic Bacteria |
| title_fullStr | Comparison of Antibacterial Activities of ProRoot MTA, OrthoMTA, and RetroMTA Against Three Anaerobic Endodontic Bacteria |
| title_full_unstemmed | Comparison of Antibacterial Activities of ProRoot MTA, OrthoMTA, and RetroMTA Against Three Anaerobic Endodontic Bacteria |
| title_short | Comparison of Antibacterial Activities of ProRoot MTA, OrthoMTA, and RetroMTA Against Three Anaerobic Endodontic Bacteria |
| title_sort | comparison of antibacterial activities of proroot mta orthomta and retromta against three anaerobic endodontic bacteria |
| topic | Bacterial Sensitivity Test Mineral Trioxide Aggregate OrthoMTA ProRoot MTA RetroMTA |
| url | https://jdt.tums.ac.ir/index.php/jdt/article/view/2404 |
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