Detecção de inibidores de proteases em sementes de Punica granatum
The use of plants as a way to prevent and treat disease comes from ancient times. With the increasing return on consumption of plants for medicinal purposes, the plant-based medicines have gained greater appreciation. Protease inhibitors are compounds that can decrease activity of an enzyme. They ar...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270&lng=en&tlng=en |
| _version_ | 1811276588387401728 |
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| author | Lara Franca Colares Antero Ricardo Santos Neto Isac Henrique Cordeiro Sandra Bertelli Ribeiro de Castro Rodrigo Moreira Verly Caio Cesar de Souza Alves Alessandra de Paula Carli |
| author_facet | Lara Franca Colares Antero Ricardo Santos Neto Isac Henrique Cordeiro Sandra Bertelli Ribeiro de Castro Rodrigo Moreira Verly Caio Cesar de Souza Alves Alessandra de Paula Carli |
| author_sort | Lara Franca Colares |
| collection | DOAJ |
| description | The use of plants as a way to prevent and treat disease comes from ancient times. With the increasing return on consumption of plants for medicinal purposes, the plant-based medicines have gained greater appreciation. Protease inhibitors are compounds that can decrease activity of an enzyme. They are found in plants, especially in Fabaceae, Poaceae, and Solanaceae families, and show satisfactory use to treat diseases due to their regulatory activities and selective proteolytic degradation of target substrates. This study aimed to obtain protease inhibitors in the seeds of Punica granatum, due its use in traditional medicine. The alcohol-acid solution was used to extract and isolate the protease inhibitors in P. granatum seeds and Glycine max grains. The extract of P. granatum seeds showed similar concentration of protease inhibitors when compared to G. max. A 14 kDa band was detected by electrophoresis on polyacrylamide gel, which was characterized by high-performance liquid chromatography as protease inhibitors. The extract of P. granatum seeds showed a potent trypsin inhibitor activity (EC50 18.2 ng mL-1). This study showed for the first time the extraction, purification, and identification of protease inhibitors in Punica granatum seeds. |
| first_indexed | 2024-04-13T00:00:30Z |
| format | Article |
| id | doaj.art-989dfb4d09b147059f27bb2118df6a08 |
| institution | Directory Open Access Journal |
| issn | 1678-7064 |
| language | English |
| last_indexed | 2024-04-13T00:00:30Z |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-989dfb4d09b147059f27bb2118df6a082022-12-22T03:11:23ZengSociedade Brasileira de QuímicaQuímica Nova1678-706440327027410.21577/0100-4042.20160189S0100-40422017000300270Detecção de inibidores de proteases em sementes de Punica granatumLara Franca ColaresAntero Ricardo Santos NetoIsac Henrique CordeiroSandra Bertelli Ribeiro de CastroRodrigo Moreira VerlyCaio Cesar de Souza AlvesAlessandra de Paula CarliThe use of plants as a way to prevent and treat disease comes from ancient times. With the increasing return on consumption of plants for medicinal purposes, the plant-based medicines have gained greater appreciation. Protease inhibitors are compounds that can decrease activity of an enzyme. They are found in plants, especially in Fabaceae, Poaceae, and Solanaceae families, and show satisfactory use to treat diseases due to their regulatory activities and selective proteolytic degradation of target substrates. This study aimed to obtain protease inhibitors in the seeds of Punica granatum, due its use in traditional medicine. The alcohol-acid solution was used to extract and isolate the protease inhibitors in P. granatum seeds and Glycine max grains. The extract of P. granatum seeds showed similar concentration of protease inhibitors when compared to G. max. A 14 kDa band was detected by electrophoresis on polyacrylamide gel, which was characterized by high-performance liquid chromatography as protease inhibitors. The extract of P. granatum seeds showed a potent trypsin inhibitor activity (EC50 18.2 ng mL-1). This study showed for the first time the extraction, purification, and identification of protease inhibitors in Punica granatum seeds.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270&lng=en&tlng=enprotease inhibitorsPunica granatum seedstraditional medicine |
| spellingShingle | Lara Franca Colares Antero Ricardo Santos Neto Isac Henrique Cordeiro Sandra Bertelli Ribeiro de Castro Rodrigo Moreira Verly Caio Cesar de Souza Alves Alessandra de Paula Carli Detecção de inibidores de proteases em sementes de Punica granatum Química Nova protease inhibitors Punica granatum seeds traditional medicine |
| title | Detecção de inibidores de proteases em sementes de Punica granatum |
| title_full | Detecção de inibidores de proteases em sementes de Punica granatum |
| title_fullStr | Detecção de inibidores de proteases em sementes de Punica granatum |
| title_full_unstemmed | Detecção de inibidores de proteases em sementes de Punica granatum |
| title_short | Detecção de inibidores de proteases em sementes de Punica granatum |
| title_sort | deteccao de inibidores de proteases em sementes de punica granatum |
| topic | protease inhibitors Punica granatum seeds traditional medicine |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000300270&lng=en&tlng=en |
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