Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma.
Tetraspanin CD151 has been implicated in metastasis through forming complexes with different molecular partners. In this study, we mapped tetraspanin web proteins centered on CD151, in order to explore the role of CD151 complexes in the progression of hepatocellular carcinoma (HCC). Immunoprecipitat...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3178554?pdf=render |
| _version_ | 1818039369378824192 |
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| author | Ranjan Prasad Devbhandari Guo-Ming Shi Ai-Wu Ke Fei-Zhen Wu Xiao-Yong Huang Xiao-Ying Wang Ying-Hong Shi Zhen-Bin Ding Yang Xu Zhi Dai Jia Fan Jian Zhou |
| author_facet | Ranjan Prasad Devbhandari Guo-Ming Shi Ai-Wu Ke Fei-Zhen Wu Xiao-Yong Huang Xiao-Ying Wang Ying-Hong Shi Zhen-Bin Ding Yang Xu Zhi Dai Jia Fan Jian Zhou |
| author_sort | Ranjan Prasad Devbhandari |
| collection | DOAJ |
| description | Tetraspanin CD151 has been implicated in metastasis through forming complexes with different molecular partners. In this study, we mapped tetraspanin web proteins centered on CD151, in order to explore the role of CD151 complexes in the progression of hepatocellular carcinoma (HCC). Immunoprecipitation was used to isolate tetraspanin complexes from HCCLM3 cells using a CD151 antibody, and associated proteins were identified by mass spectrometry. The interaction of CD151 and its molecular partners, and their roles in invasiveness and metastasis of HCC cells were assayed through disruption of the CD151 network. Finally, the clinical implication of CD151 complexes in HCC patients was also examined. In this study, we identified 58 proteins, characterized the tetraspanin CD151 web, and chose integrin β1 as a main partner to further investigate. When the CD151/integrin β1 complex in HCC cells was disrupted, migration, invasiveness, secretion of matrix metalloproteinase 9, and metastasis were markedly influenced. However, both CD151 and integrin β1 expression were untouched. HCC patients with high expression of CD151/integrin β1 complex had the poorest prognosis of the whole cohort of patients. Together, our data show that CD151 acts as an important player in the progression of HCC in an integrin β1-dependent manner. |
| first_indexed | 2024-12-10T07:57:32Z |
| format | Article |
| id | doaj.art-989e070ac71244bd810bc869d9d172fb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T07:57:32Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-989e070ac71244bd810bc869d9d172fb2022-12-22T01:56:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2490110.1371/journal.pone.0024901Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma.Ranjan Prasad DevbhandariGuo-Ming ShiAi-Wu KeFei-Zhen WuXiao-Yong HuangXiao-Ying WangYing-Hong ShiZhen-Bin DingYang XuZhi DaiJia FanJian ZhouTetraspanin CD151 has been implicated in metastasis through forming complexes with different molecular partners. In this study, we mapped tetraspanin web proteins centered on CD151, in order to explore the role of CD151 complexes in the progression of hepatocellular carcinoma (HCC). Immunoprecipitation was used to isolate tetraspanin complexes from HCCLM3 cells using a CD151 antibody, and associated proteins were identified by mass spectrometry. The interaction of CD151 and its molecular partners, and their roles in invasiveness and metastasis of HCC cells were assayed through disruption of the CD151 network. Finally, the clinical implication of CD151 complexes in HCC patients was also examined. In this study, we identified 58 proteins, characterized the tetraspanin CD151 web, and chose integrin β1 as a main partner to further investigate. When the CD151/integrin β1 complex in HCC cells was disrupted, migration, invasiveness, secretion of matrix metalloproteinase 9, and metastasis were markedly influenced. However, both CD151 and integrin β1 expression were untouched. HCC patients with high expression of CD151/integrin β1 complex had the poorest prognosis of the whole cohort of patients. Together, our data show that CD151 acts as an important player in the progression of HCC in an integrin β1-dependent manner.http://europepmc.org/articles/PMC3178554?pdf=render |
| spellingShingle | Ranjan Prasad Devbhandari Guo-Ming Shi Ai-Wu Ke Fei-Zhen Wu Xiao-Yong Huang Xiao-Ying Wang Ying-Hong Shi Zhen-Bin Ding Yang Xu Zhi Dai Jia Fan Jian Zhou Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma. PLoS ONE |
| title | Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma. |
| title_full | Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma. |
| title_fullStr | Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma. |
| title_full_unstemmed | Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma. |
| title_short | Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma. |
| title_sort | profiling of the tetraspanin cd151 web and conspiracy of cd151 integrin β1 complex in the progression of hepatocellular carcinoma |
| url | http://europepmc.org/articles/PMC3178554?pdf=render |
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