Testing of Auxotrophic Selection Markers for Use in the Moss Physcomitrella Provides New Insights into the Mechanisms of Targeted Recombination
The moss Physcomitrella patens is unique among plants in that homologous recombination can be used to knock out genes, just like in yeast. Furthermore, transformed plasmids can be rescued from Physcomitrella back into Escherichia coli, similar to yeast. In the present study, we have tested if a thir...
Main Authors: | Mikael Ulfstedt, Guo-Zhen Hu, Monika Johansson, Hans Ronne |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2017-11-01
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Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01850/full |
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