No Evidence for Light-Induced Embolism Repair in Cut Stems of Drought-Resistant Mediterranean Species under Soaking
(1) Recent studies suggested that stem photosynthesis could favor bark water uptake and embolism recovery when stem segments are soaked in water under light conditions, but evidence for this phenomenon in drought-resistant Mediterranean species with photosynthetic stems is missing. (2) Embolism reco...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/3/307 |
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| author | Martina Tomasella Sara Natale Francesco Petruzzellis Sara Di Bert Lorenzo D’Amico Giuliana Tromba Andrea Nardini |
| author_facet | Martina Tomasella Sara Natale Francesco Petruzzellis Sara Di Bert Lorenzo D’Amico Giuliana Tromba Andrea Nardini |
| author_sort | Martina Tomasella |
| collection | DOAJ |
| description | (1) Recent studies suggested that stem photosynthesis could favor bark water uptake and embolism recovery when stem segments are soaked in water under light conditions, but evidence for this phenomenon in drought-resistant Mediterranean species with photosynthetic stems is missing. (2) Embolism recovery upon immersion in water for 2 h–4 h under light was assessed (i) via a classical hydraulic method in leafless <i>Fraxinus ornus</i> and <i>Olea europaea</i> branch segments stressed to xylem water potentials (Y<sub>xyl</sub>) inducing ca. 50% loss of hydraulic conductivity (PLC) and (ii) via X-ray micro-CT imaging of the stem segments of drought-stressed potted <i>F. ornus</i> saplings. Hydraulic recovery was also assessed in vivo in intact drought-stressed <i>F. ornus</i> saplings upon soil re-irrigation. (3) Intact <i>F. ornus</i> plants recovered hydraulic function through root water uptake. Conversely, the soaked stem segments of both species did not refill embolized conduits, although Y<sub>xyl </sub>recovered to pre-stress levels (between −0.5 MPa and −0.2 MPa). (4) We hypothesize that xylem embolism recovery through bark water uptake, even in light conditions, may not be a common phenomenon in woody plants and/or that wounds caused by cutting short stem segments might inhibit the refilling process upon soaking. |
| first_indexed | 2024-03-09T23:19:42Z |
| format | Article |
| id | doaj.art-98a3b24515984315b61418e7863302f0 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T23:19:42Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-98a3b24515984315b61418e7863302f02023-11-23T17:29:46ZengMDPI AGPlants2223-77472022-01-0111330710.3390/plants11030307No Evidence for Light-Induced Embolism Repair in Cut Stems of Drought-Resistant Mediterranean Species under SoakingMartina Tomasella0Sara Natale1Francesco Petruzzellis2Sara Di Bert3Lorenzo D’Amico4Giuliana Tromba5Andrea Nardini6Dipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127 Trieste, ItalyDipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127 Trieste, ItalyDipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127 Trieste, ItalyDipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127 Trieste, ItalyElettra-Sincrotrone Trieste, Area Science Park, 34149, Basovizza, ItalyElettra-Sincrotrone Trieste, Area Science Park, 34149, Basovizza, ItalyDipartimento di Scienze della Vita, Università di Trieste, Via L. Giorgieri 10, 34127 Trieste, Italy(1) Recent studies suggested that stem photosynthesis could favor bark water uptake and embolism recovery when stem segments are soaked in water under light conditions, but evidence for this phenomenon in drought-resistant Mediterranean species with photosynthetic stems is missing. (2) Embolism recovery upon immersion in water for 2 h–4 h under light was assessed (i) via a classical hydraulic method in leafless <i>Fraxinus ornus</i> and <i>Olea europaea</i> branch segments stressed to xylem water potentials (Y<sub>xyl</sub>) inducing ca. 50% loss of hydraulic conductivity (PLC) and (ii) via X-ray micro-CT imaging of the stem segments of drought-stressed potted <i>F. ornus</i> saplings. Hydraulic recovery was also assessed in vivo in intact drought-stressed <i>F. ornus</i> saplings upon soil re-irrigation. (3) Intact <i>F. ornus</i> plants recovered hydraulic function through root water uptake. Conversely, the soaked stem segments of both species did not refill embolized conduits, although Y<sub>xyl </sub>recovered to pre-stress levels (between −0.5 MPa and −0.2 MPa). (4) We hypothesize that xylem embolism recovery through bark water uptake, even in light conditions, may not be a common phenomenon in woody plants and/or that wounds caused by cutting short stem segments might inhibit the refilling process upon soaking.https://www.mdpi.com/2223-7747/11/3/307stem photosynthesishydraulic recoverysoakingX-ray micro-CTbark water uptakeembolism |
| spellingShingle | Martina Tomasella Sara Natale Francesco Petruzzellis Sara Di Bert Lorenzo D’Amico Giuliana Tromba Andrea Nardini No Evidence for Light-Induced Embolism Repair in Cut Stems of Drought-Resistant Mediterranean Species under Soaking Plants stem photosynthesis hydraulic recovery soaking X-ray micro-CT bark water uptake embolism |
| title | No Evidence for Light-Induced Embolism Repair in Cut Stems of Drought-Resistant Mediterranean Species under Soaking |
| title_full | No Evidence for Light-Induced Embolism Repair in Cut Stems of Drought-Resistant Mediterranean Species under Soaking |
| title_fullStr | No Evidence for Light-Induced Embolism Repair in Cut Stems of Drought-Resistant Mediterranean Species under Soaking |
| title_full_unstemmed | No Evidence for Light-Induced Embolism Repair in Cut Stems of Drought-Resistant Mediterranean Species under Soaking |
| title_short | No Evidence for Light-Induced Embolism Repair in Cut Stems of Drought-Resistant Mediterranean Species under Soaking |
| title_sort | no evidence for light induced embolism repair in cut stems of drought resistant mediterranean species under soaking |
| topic | stem photosynthesis hydraulic recovery soaking X-ray micro-CT bark water uptake embolism |
| url | https://www.mdpi.com/2223-7747/11/3/307 |
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