Dependency of B-1 Cells in the Maintenance of Splenic Interleukin-10 Producing Cells and Impairment of Macrophage Resistance in Visceral Leishmaniasis
Visceral leishmaniasis is a neglected disease caused by Leishmania protozoa parasites transmitted by infected sand fly vectors. This disease represents the second in mortality among tropical infections and is associated to a profound immunosuppression state of the host. The hallmark of this infectio...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00978/full |
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| author | Angélica Fernandes Arcanjo Dirlei Nico Gabriellen Menezes Migliani de Castro Yasmin da Silva Fontes Paula Saltarelli Debora Decote-Ricardo Marise P. Nunes Antônio Ferreira-Pereira Clarisa B. Palatnik-de-Sousa Célio G. Freire-de-Lima Alexandre Morrot Alexandre Morrot |
| author_facet | Angélica Fernandes Arcanjo Dirlei Nico Gabriellen Menezes Migliani de Castro Yasmin da Silva Fontes Paula Saltarelli Debora Decote-Ricardo Marise P. Nunes Antônio Ferreira-Pereira Clarisa B. Palatnik-de-Sousa Célio G. Freire-de-Lima Alexandre Morrot Alexandre Morrot |
| author_sort | Angélica Fernandes Arcanjo |
| collection | DOAJ |
| description | Visceral leishmaniasis is a neglected disease caused by Leishmania protozoa parasites transmitted by infected sand fly vectors. This disease represents the second in mortality among tropical infections and is associated to a profound immunosuppression state of the host. The hallmark of this infection-induced host immunodeviation is the characteristic high levels of the regulatory interleukin-10 (IL-10) cytokine. In the present study, we investigated the role of B-1 cells in the maintenance of splenic IL-10 levels that could interfere with resistance to parasite infection. Using an experimental murine infection model with Leishmania (L.) infantum chagasi we demonstrated an improved resistance of B-1 deficient BALB/XID mice to infection. BALB/XID mice developed a reduced splenomegaly with diminished splenic parasite burden and lower levels of IL-10 secretion of purified splenocytes at 30 days post-infection, as compared to BALB/c wild-type control mice. Interestingly, we found that resident peritoneal macrophages isolated from BALB/XID mice were more effective to control the parasite load in comparison to cells isolated from BALB/c wild-type mice. Our findings point to a role of B-1 cells in the host susceptibility to visceral leishmaniasis. |
| first_indexed | 2024-12-11T00:53:55Z |
| format | Article |
| id | doaj.art-98a3c68a99a849fea83f28554a3785ba |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T00:53:55Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-98a3c68a99a849fea83f28554a3785ba2022-12-22T01:26:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-06-01810.3389/fmicb.2017.00978221453Dependency of B-1 Cells in the Maintenance of Splenic Interleukin-10 Producing Cells and Impairment of Macrophage Resistance in Visceral LeishmaniasisAngélica Fernandes Arcanjo0Dirlei Nico1Gabriellen Menezes Migliani de Castro2Yasmin da Silva Fontes3Paula Saltarelli4Debora Decote-Ricardo5Marise P. Nunes6Antônio Ferreira-Pereira7Clarisa B. Palatnik-de-Sousa8Célio G. Freire-de-Lima9Alexandre Morrot10Alexandre Morrot11Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilDepartamento de Microbiologia Geral, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilDepartamento de Microbiologia Geral, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilDepartamento de Microbiologia Geral, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilDepartamento de Microbiologia Geral, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilInstituto de Veterinária, Universidade Federal Rural do Rio de JaneiroRio de Janeiro, BrazilInstituto Oswaldo Cruz, FiocruzRio de Janeiro, BrazilDepartamento de Microbiologia Geral, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilDepartamento de Microbiologia Geral, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilInstituto Oswaldo Cruz, FiocruzRio de Janeiro, BrazilDepartamento de Imunologia, Instituto de Microbiologia, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilVisceral leishmaniasis is a neglected disease caused by Leishmania protozoa parasites transmitted by infected sand fly vectors. This disease represents the second in mortality among tropical infections and is associated to a profound immunosuppression state of the host. The hallmark of this infection-induced host immunodeviation is the characteristic high levels of the regulatory interleukin-10 (IL-10) cytokine. In the present study, we investigated the role of B-1 cells in the maintenance of splenic IL-10 levels that could interfere with resistance to parasite infection. Using an experimental murine infection model with Leishmania (L.) infantum chagasi we demonstrated an improved resistance of B-1 deficient BALB/XID mice to infection. BALB/XID mice developed a reduced splenomegaly with diminished splenic parasite burden and lower levels of IL-10 secretion of purified splenocytes at 30 days post-infection, as compared to BALB/c wild-type control mice. Interestingly, we found that resident peritoneal macrophages isolated from BALB/XID mice were more effective to control the parasite load in comparison to cells isolated from BALB/c wild-type mice. Our findings point to a role of B-1 cells in the host susceptibility to visceral leishmaniasis.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00978/fullvisceral leishmaniasisLeishmania (L.) infantum chagasiB-1 cellshost protective responses |
| spellingShingle | Angélica Fernandes Arcanjo Dirlei Nico Gabriellen Menezes Migliani de Castro Yasmin da Silva Fontes Paula Saltarelli Debora Decote-Ricardo Marise P. Nunes Antônio Ferreira-Pereira Clarisa B. Palatnik-de-Sousa Célio G. Freire-de-Lima Alexandre Morrot Alexandre Morrot Dependency of B-1 Cells in the Maintenance of Splenic Interleukin-10 Producing Cells and Impairment of Macrophage Resistance in Visceral Leishmaniasis Frontiers in Microbiology visceral leishmaniasis Leishmania (L.) infantum chagasi B-1 cells host protective responses |
| title | Dependency of B-1 Cells in the Maintenance of Splenic Interleukin-10 Producing Cells and Impairment of Macrophage Resistance in Visceral Leishmaniasis |
| title_full | Dependency of B-1 Cells in the Maintenance of Splenic Interleukin-10 Producing Cells and Impairment of Macrophage Resistance in Visceral Leishmaniasis |
| title_fullStr | Dependency of B-1 Cells in the Maintenance of Splenic Interleukin-10 Producing Cells and Impairment of Macrophage Resistance in Visceral Leishmaniasis |
| title_full_unstemmed | Dependency of B-1 Cells in the Maintenance of Splenic Interleukin-10 Producing Cells and Impairment of Macrophage Resistance in Visceral Leishmaniasis |
| title_short | Dependency of B-1 Cells in the Maintenance of Splenic Interleukin-10 Producing Cells and Impairment of Macrophage Resistance in Visceral Leishmaniasis |
| title_sort | dependency of b 1 cells in the maintenance of splenic interleukin 10 producing cells and impairment of macrophage resistance in visceral leishmaniasis |
| topic | visceral leishmaniasis Leishmania (L.) infantum chagasi B-1 cells host protective responses |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00978/full |
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