Effects of tea garden soil on aroma components and related gene expression in tea leaves
In order to explore the effect of soil on the synthesis of aroma components in tea leaves, tea seedlings replanted in tea rhizosphere soil of different ages were used as research materials. Tea seedlings were replanted in soils aged 0, 4, 9, and 30 years, and after one year of growth, 34, 37, 29, an...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Julius Kühn-Institut
2020-06-01
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| Series: | Journal of Applied Botany and Food Quality |
| Online Access: | https://ojs.openagrar.de/index.php/JABFQ/article/view/13382 |
| _version_ | 1798017382733578240 |
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| author | Haibin Wang Xiaoting Chen Yuhua Wang Jianghua Ye Xiao-li Jia Qi Zhang Haibin He |
| author_facet | Haibin Wang Xiaoting Chen Yuhua Wang Jianghua Ye Xiao-li Jia Qi Zhang Haibin He |
| author_sort | Haibin Wang |
| collection | DOAJ |
| description | In order to explore the effect of soil on the synthesis of aroma components in tea leaves, tea seedlings replanted in tea rhizosphere soil of different ages were used as research materials. Tea seedlings were replanted in soils aged 0, 4, 9, and 30 years, and after one year of growth, 34, 37, 29, and 26 substances were detected in the tea leaves, respectively, using gas chromatography-mass spectrometry (GC-MS). The relative contents of terpenoids and alcohols in the tea leaves dropped from 66.40% to 44.52% and 5.21% to 2.61%, respectively, as the age of the rhizosphere soil increased. Aldehydes, esters, and nitrogen compounds increased from 3.80% to 22.36%, 1.33% to 12.02%, and 3.13% to 19.96%, respectively, as the age of the rhizosphere soil increased. Gene differential expression measured by fluorescence quantitative PCR (qRT-PCR) showed that the number of nerolidol synthetase and linalool synthase genes in tea leaves increased significantly, and the terpineol synthetase, phellandrene synthase, myrcene synthetase, ocimene synthase, limonene synthetase, germacrene synthase, and farnesene synthase genes declined significantly with the increase in soil age. In summary, as the number of years tea had been planted in the soil increased, the soil significantly affected the expression of terpene synthase genes in tea leaves, and then the composition and content of aroma substances in tea leaves changed. The results provide a theoretical basis for the improvement of tea quality. |
| first_indexed | 2024-04-11T16:06:23Z |
| format | Article |
| id | doaj.art-98aa29c17b3f4709802dfabdf20f06a9 |
| institution | Directory Open Access Journal |
| issn | 1613-9216 1439-040X |
| language | English |
| last_indexed | 2024-04-11T16:06:23Z |
| publishDate | 2020-06-01 |
| publisher | Julius Kühn-Institut |
| record_format | Article |
| series | Journal of Applied Botany and Food Quality |
| spelling | doaj.art-98aa29c17b3f4709802dfabdf20f06a92022-12-22T04:14:49ZengJulius Kühn-InstitutJournal of Applied Botany and Food Quality1613-92161439-040X2020-06-019310.5073/JABFQ.2020.093.013Effects of tea garden soil on aroma components and related gene expression in tea leavesHaibin Wang0Xiaoting Chen1Yuhua Wang2Jianghua Ye3Xiao-li Jia4Qi Zhang5Haibin He6College of Life Sciences, Longyan University, Longyan, ChinaCollege of Life Sciences, Longyan University, Longyan, ChinaCollege of Life Sciences, Longyan University, Longyan, ChinaCollege of Tea and Food, Wuyi University, Wuyishan, ChinaCollege of Tea and Food, Wuyi University, Wuyishan, ChinaCollege of Tea and Food, Wuyi University, Wuyishan, ChinaFujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou, ChinaIn order to explore the effect of soil on the synthesis of aroma components in tea leaves, tea seedlings replanted in tea rhizosphere soil of different ages were used as research materials. Tea seedlings were replanted in soils aged 0, 4, 9, and 30 years, and after one year of growth, 34, 37, 29, and 26 substances were detected in the tea leaves, respectively, using gas chromatography-mass spectrometry (GC-MS). The relative contents of terpenoids and alcohols in the tea leaves dropped from 66.40% to 44.52% and 5.21% to 2.61%, respectively, as the age of the rhizosphere soil increased. Aldehydes, esters, and nitrogen compounds increased from 3.80% to 22.36%, 1.33% to 12.02%, and 3.13% to 19.96%, respectively, as the age of the rhizosphere soil increased. Gene differential expression measured by fluorescence quantitative PCR (qRT-PCR) showed that the number of nerolidol synthetase and linalool synthase genes in tea leaves increased significantly, and the terpineol synthetase, phellandrene synthase, myrcene synthetase, ocimene synthase, limonene synthetase, germacrene synthase, and farnesene synthase genes declined significantly with the increase in soil age. In summary, as the number of years tea had been planted in the soil increased, the soil significantly affected the expression of terpene synthase genes in tea leaves, and then the composition and content of aroma substances in tea leaves changed. The results provide a theoretical basis for the improvement of tea quality.https://ojs.openagrar.de/index.php/JABFQ/article/view/13382 |
| spellingShingle | Haibin Wang Xiaoting Chen Yuhua Wang Jianghua Ye Xiao-li Jia Qi Zhang Haibin He Effects of tea garden soil on aroma components and related gene expression in tea leaves Journal of Applied Botany and Food Quality |
| title | Effects of tea garden soil on aroma components and related gene expression in tea leaves |
| title_full | Effects of tea garden soil on aroma components and related gene expression in tea leaves |
| title_fullStr | Effects of tea garden soil on aroma components and related gene expression in tea leaves |
| title_full_unstemmed | Effects of tea garden soil on aroma components and related gene expression in tea leaves |
| title_short | Effects of tea garden soil on aroma components and related gene expression in tea leaves |
| title_sort | effects of tea garden soil on aroma components and related gene expression in tea leaves |
| url | https://ojs.openagrar.de/index.php/JABFQ/article/view/13382 |
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