Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein

Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein

Abstract Guanidinocalix[5]arene and fluorescein reporter pair has been chosen to set up a supramolecular tandem assay principle based on the differential recognition of pyridoxal‐5′‐phosphate (the substrate of alkaline phosphatase, ALP), pyridoxal (the product of ALP) and phosphate (the product of A...

Full description

Bibliographic Details
Main Authors: Yu‐Xin Yue, Yong Kong, Fan Yang, Zhe Zheng, Xin‐Yue Hu, Dong‐Sheng Guo
Format: Article
Language:English
Published: Wiley-VCH 2019-12-01
Series:ChemistryOpen
Subjects:
macrocyclic chemistry
calixarenes
fluorescence sensing
supramolecular tandem assay
pyridoxal-5′-phosphate
Online Access:https://doi.org/10.1002/open.201900316
Description
Summary:Abstract Guanidinocalix[5]arene and fluorescein reporter pair has been chosen to set up a supramolecular tandem assay principle based on the differential recognition of pyridoxal‐5′‐phosphate (the substrate of alkaline phosphatase, ALP), pyridoxal (the product of ALP) and phosphate (the product of ALP). This supramolecular tandem assay system offers an opportunity to monitor the activity of ALP in a label‐free, continuous, and real‐time manner. More importantly, a calibration curve can be given for selective and quantitative detection of pyridoxal‐5′‐phosphate (biomarker for several diseases).
ISSN:2191-1363

Similar Items