NG-Nitro-L-arginine Methyl Ester-Induced Potentiaton of the Effect of Aminophylline on Rat Diaphragm: the Role of Extracellular Calcium
The role of extracellular calcium in the interaction between intracellular cAMP and nitric oxide (NO)/cGMP on the contractility of rat diaphragm pretreated with cumulative concentrations of aminophylline (0.36 – 3.60 mM) was investigated. In a Ca2+-free medium, NG-nitro-L-arginine methyl ester (L-NA...
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| Format: | Article |
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Elsevier
2004-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319323291 |
| _version_ | 1818059161314787328 |
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| author | Radan Stojanović Zoran Todorović Zorica Nešić Sonja Vućković Nataša Cerovac-ćosić Milica Prostran |
| author_facet | Radan Stojanović Zoran Todorović Zorica Nešić Sonja Vućković Nataša Cerovac-ćosić Milica Prostran |
| author_sort | Radan Stojanović |
| collection | DOAJ |
| description | The role of extracellular calcium in the interaction between intracellular cAMP and nitric oxide (NO)/cGMP on the contractility of rat diaphragm pretreated with cumulative concentrations of aminophylline (0.36 – 3.60 mM) was investigated. In a Ca2+-free medium, NG-nitro-L-arginine methyl ester (L-NAME) (1 and 3 mM) depressed tension developed (Td) and also aminophylline-induced potentiation of Td in a concentration-dependent manner. Verapamil (2.5 μM) or nicardipine (20 μM) significantly antagonized the potentiating effect of L-NAME on Td in a calcium-containing medium. However, in the presence of verapamil or nicardipine, L-NAME still produced statistically significant potentiation of the cumulative concentrations of aminophylline (0.36 – 3.60 mM), given in the second series. Keywords:: aminophylline, calcium, NG-nitro-L-arginine methyl ester |
| first_indexed | 2024-12-10T13:12:07Z |
| format | Article |
| id | doaj.art-98b346b060994d18807163fab63ba9cc |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-10T13:12:07Z |
| publishDate | 2004-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-98b346b060994d18807163fab63ba9cc2022-12-22T01:47:38ZengElsevierJournal of Pharmacological Sciences1347-86132004-01-01964493498NG-Nitro-L-arginine Methyl Ester-Induced Potentiaton of the Effect of Aminophylline on Rat Diaphragm: the Role of Extracellular CalciumRadan Stojanović0Zoran Todorović1Zorica Nešić2Sonja Vućković3Nataša Cerovac-ćosić4Milica Prostran5Department of Clinical Pharmacology, Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, Serbia and MontenegroDepartment of Clinical Pharmacology, Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, Serbia and MontenegroDepartment of Clinical Pharmacology, Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, Serbia and MontenegroDepartment of Clinical Pharmacology, Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, Serbia and MontenegroDepartment of Clinical Pharmacology, Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, Serbia and MontenegroDepartment of Clinical Pharmacology, Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, Serbia and Montenegro; Corresponding author. FAX: +381-11-686-025 E-mail: mprostran@doctor.comThe role of extracellular calcium in the interaction between intracellular cAMP and nitric oxide (NO)/cGMP on the contractility of rat diaphragm pretreated with cumulative concentrations of aminophylline (0.36 – 3.60 mM) was investigated. In a Ca2+-free medium, NG-nitro-L-arginine methyl ester (L-NAME) (1 and 3 mM) depressed tension developed (Td) and also aminophylline-induced potentiation of Td in a concentration-dependent manner. Verapamil (2.5 μM) or nicardipine (20 μM) significantly antagonized the potentiating effect of L-NAME on Td in a calcium-containing medium. However, in the presence of verapamil or nicardipine, L-NAME still produced statistically significant potentiation of the cumulative concentrations of aminophylline (0.36 – 3.60 mM), given in the second series. Keywords:: aminophylline, calcium, NG-nitro-L-arginine methyl esterhttp://www.sciencedirect.com/science/article/pii/S1347861319323291 |
| spellingShingle | Radan Stojanović Zoran Todorović Zorica Nešić Sonja Vućković Nataša Cerovac-ćosić Milica Prostran NG-Nitro-L-arginine Methyl Ester-Induced Potentiaton of the Effect of Aminophylline on Rat Diaphragm: the Role of Extracellular Calcium Journal of Pharmacological Sciences |
| title | NG-Nitro-L-arginine Methyl Ester-Induced Potentiaton of the Effect of Aminophylline on Rat Diaphragm: the Role of Extracellular Calcium |
| title_full | NG-Nitro-L-arginine Methyl Ester-Induced Potentiaton of the Effect of Aminophylline on Rat Diaphragm: the Role of Extracellular Calcium |
| title_fullStr | NG-Nitro-L-arginine Methyl Ester-Induced Potentiaton of the Effect of Aminophylline on Rat Diaphragm: the Role of Extracellular Calcium |
| title_full_unstemmed | NG-Nitro-L-arginine Methyl Ester-Induced Potentiaton of the Effect of Aminophylline on Rat Diaphragm: the Role of Extracellular Calcium |
| title_short | NG-Nitro-L-arginine Methyl Ester-Induced Potentiaton of the Effect of Aminophylline on Rat Diaphragm: the Role of Extracellular Calcium |
| title_sort | ng nitro l arginine methyl ester induced potentiaton of the effect of aminophylline on rat diaphragm the role of extracellular calcium |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319323291 |
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