Prognostic Significance of DNA Topoisomerase II Alpha (TOP2A) in Cholangiocarcinoma
Background: Cholangiocarcinoma (CCA) is a malignant tumor with an increasing incidence worldwide. Although radiation therapy has improved the therapeutic efficiency of CCA treatment, differential expression of genes among cholangiocarcinoma subtypes has been revealed through precise sequencing. Howe...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IMR Press
2023-04-01
|
| Series: | Frontiers in Bioscience-Landmark |
| Subjects: | |
| Online Access: | https://www.imrpress.com/journal/FBL/28/4/10.31083/j.fbl2804075 |
| _version_ | 1797837652063420416 |
|---|---|
| author | Khaa Hoo Ong Hong-Yue Lai Ding-Ping Sun Tzu-Ju Chen Steven Kuan-Hua Huang Yu-Feng Tian Chia-Lin Chou Yow-Ling Shiue Ti-Chun Chan Chien-Feng Li Yu-Hsuan Kuo |
| author_facet | Khaa Hoo Ong Hong-Yue Lai Ding-Ping Sun Tzu-Ju Chen Steven Kuan-Hua Huang Yu-Feng Tian Chia-Lin Chou Yow-Ling Shiue Ti-Chun Chan Chien-Feng Li Yu-Hsuan Kuo |
| author_sort | Khaa Hoo Ong |
| collection | DOAJ |
| description | Background: Cholangiocarcinoma (CCA) is a malignant tumor with an increasing incidence worldwide. Although radiation therapy has improved the therapeutic efficiency of CCA treatment, differential expression of genes among cholangiocarcinoma subtypes has been revealed through precise sequencing. However, no specific molecular therapeutic targets or biomarkers have been figured out for use in precision medicine, and the exact mechanism by which antitumorigenic effects occur is still unclear. Therefore, it is necessary to conduct further studies on the development and mechanisms associated with CCA. Methods: We examined the clinical data and pathological features of patients with cholangiocarcinomas. We investigated the associations between DNA Topoisomerase II Alpha (TOP2A) expression and patient outcomes, such as metastasis-free survival (MFS) and disease-specific survival (DSS), as well as clinical characteristics and pathological results. Results: TOP2A expression was shown to be upregulated in CCA tissue sections by immunohistochemistry staining and data mining. Moreover, we observed that the TOP2A expression correlated with clinical features, such as the primary tumor stage, histological variants, and patients with hepatitis. Furthermore, high expression of TOP2A was associated with worse survival outcomes in terms of the overall survival (p < 0.0001), disease-specific survival (p < 0.0001), and metastasis-free survival (p < 0.0001) compared with patients in the low TOP2A expression group. This indicates that a high level of TOP2A expression is related to an unfavorable prognosis. Conclusions: Our results show that TOP2A is highly expressed in CCA tissues, and its upregulation is correlated with the primary disease stage and poor prognosis significantly. Consequently, TOP2A is a prognostic biomarker and a novel therapeutic target for the treatment of CCA. |
| first_indexed | 2024-04-09T15:29:14Z |
| format | Article |
| id | doaj.art-98b7e14fc15147e293eede3e99fbbc6f |
| institution | Directory Open Access Journal |
| issn | 2768-6701 |
| language | English |
| last_indexed | 2024-04-09T15:29:14Z |
| publishDate | 2023-04-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj.art-98b7e14fc15147e293eede3e99fbbc6f2023-04-28T09:24:06ZengIMR PressFrontiers in Bioscience-Landmark2768-67012023-04-012847510.31083/j.fbl2804075S2768-6701(23)00851-1Prognostic Significance of DNA Topoisomerase II Alpha (TOP2A) in CholangiocarcinomaKhaa Hoo Ong0Hong-Yue Lai1Ding-Ping Sun2Tzu-Ju Chen3Steven Kuan-Hua Huang4Yu-Feng Tian5Chia-Lin Chou6Yow-Ling Shiue7Ti-Chun Chan8Chien-Feng Li9Yu-Hsuan Kuo10Division of Gastroenterology & General Surgery, Department of Surgery, Chi Mei Medical Center, 710 Tainan, TaiwanDepartment of Pharmacology, School of Medicine, China Medical University, 404333 Taichung, TaiwanDivision of Gastroenterology & General Surgery, Department of Surgery, Chi Mei Medical Center, 710 Tainan, TaiwanDepartment of Medical Technology, Chung Hwa University of Medical Technology, 717 Tainan, TaiwanDivision of Urology, Department of Surgery, Chi Mei Medical Center, 710 Tainan, TaiwanDivision of Colon and Rectal Surgery, Department of Surgery, Chi Mei Medical Center, 710 Tainan, TaiwanDepartment of Medical Technology, Chung Hwa University of Medical Technology, 717 Tainan, TaiwanInstitute of Biomedical Sciences, National Sun Yat-sen University, 804 Kaohsiung, TaiwanDepartment of Medical Research, Chi Mei Medical Center, 710 Tainan, TaiwanInstitute of Precision Medicine, National Sun Yat-sen University, 804 Kaohsiung, TaiwanInstitute of Biomedical Sciences, National Sun Yat-sen University, 804 Kaohsiung, TaiwanBackground: Cholangiocarcinoma (CCA) is a malignant tumor with an increasing incidence worldwide. Although radiation therapy has improved the therapeutic efficiency of CCA treatment, differential expression of genes among cholangiocarcinoma subtypes has been revealed through precise sequencing. However, no specific molecular therapeutic targets or biomarkers have been figured out for use in precision medicine, and the exact mechanism by which antitumorigenic effects occur is still unclear. Therefore, it is necessary to conduct further studies on the development and mechanisms associated with CCA. Methods: We examined the clinical data and pathological features of patients with cholangiocarcinomas. We investigated the associations between DNA Topoisomerase II Alpha (TOP2A) expression and patient outcomes, such as metastasis-free survival (MFS) and disease-specific survival (DSS), as well as clinical characteristics and pathological results. Results: TOP2A expression was shown to be upregulated in CCA tissue sections by immunohistochemistry staining and data mining. Moreover, we observed that the TOP2A expression correlated with clinical features, such as the primary tumor stage, histological variants, and patients with hepatitis. Furthermore, high expression of TOP2A was associated with worse survival outcomes in terms of the overall survival (p < 0.0001), disease-specific survival (p < 0.0001), and metastasis-free survival (p < 0.0001) compared with patients in the low TOP2A expression group. This indicates that a high level of TOP2A expression is related to an unfavorable prognosis. Conclusions: Our results show that TOP2A is highly expressed in CCA tissues, and its upregulation is correlated with the primary disease stage and poor prognosis significantly. Consequently, TOP2A is a prognostic biomarker and a novel therapeutic target for the treatment of CCA.https://www.imrpress.com/journal/FBL/28/4/10.31083/j.fbl2804075cholangiocarcinomadna topoisomerase (atp-hydrolyzing activity) activitytopoisomerase ii α (top2a)prognostic biomarker |
| spellingShingle | Khaa Hoo Ong Hong-Yue Lai Ding-Ping Sun Tzu-Ju Chen Steven Kuan-Hua Huang Yu-Feng Tian Chia-Lin Chou Yow-Ling Shiue Ti-Chun Chan Chien-Feng Li Yu-Hsuan Kuo Prognostic Significance of DNA Topoisomerase II Alpha (TOP2A) in Cholangiocarcinoma Frontiers in Bioscience-Landmark cholangiocarcinoma dna topoisomerase (atp-hydrolyzing activity) activity topoisomerase ii α (top2a) prognostic biomarker |
| title | Prognostic Significance of DNA Topoisomerase II Alpha (TOP2A) in Cholangiocarcinoma |
| title_full | Prognostic Significance of DNA Topoisomerase II Alpha (TOP2A) in Cholangiocarcinoma |
| title_fullStr | Prognostic Significance of DNA Topoisomerase II Alpha (TOP2A) in Cholangiocarcinoma |
| title_full_unstemmed | Prognostic Significance of DNA Topoisomerase II Alpha (TOP2A) in Cholangiocarcinoma |
| title_short | Prognostic Significance of DNA Topoisomerase II Alpha (TOP2A) in Cholangiocarcinoma |
| title_sort | prognostic significance of dna topoisomerase ii alpha top2a in cholangiocarcinoma |
| topic | cholangiocarcinoma dna topoisomerase (atp-hydrolyzing activity) activity topoisomerase ii α (top2a) prognostic biomarker |
| url | https://www.imrpress.com/journal/FBL/28/4/10.31083/j.fbl2804075 |
| work_keys_str_mv | AT khaahooong prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma AT hongyuelai prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma AT dingpingsun prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma AT tzujuchen prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma AT stevenkuanhuahuang prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma AT yufengtian prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma AT chialinchou prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma AT yowlingshiue prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma AT tichunchan prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma AT chienfengli prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma AT yuhsuankuo prognosticsignificanceofdnatopoisomeraseiialphatop2aincholangiocarcinoma |