Diversity of Amoeba-Associated Giant Viruses Isolated in Algeria
The discovery of several giant amoeba viruses has opened up a novel area in the field of virology. Despite this, knowledge about ecology of these viruses remains patchy. In this study, we aimed to characterize the diversity of giant viruses in Algeria by inoculating 64 environmental samples on vario...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Diversity |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-2818/12/6/215 |
| _version_ | 1797566675836469248 |
|---|---|
| author | Hadjer Boudjemaa Julien Andreani Idir Bitam Bernard La Scola |
| author_facet | Hadjer Boudjemaa Julien Andreani Idir Bitam Bernard La Scola |
| author_sort | Hadjer Boudjemaa |
| collection | DOAJ |
| description | The discovery of several giant amoeba viruses has opened up a novel area in the field of virology. Despite this, knowledge about ecology of these viruses remains patchy. In this study, we aimed to characterize the diversity of giant viruses in Algeria by inoculating 64 environmental samples on various amoeba strains. After isolation by co-culture with nine amoeba supports, flow cytometry and electron microscopy were used to putatively identify viruses. Definitive identification was performed by PCR and sequencing. Mimiviruses, marseilleviruses, faustoviruses and cedratviruses were the main viruses isolated in this study. Moreover, a new virus, which we named fadolivirus, was also isolated and was found to belong to the recent metagenomic descriptions of Klosneuvirinae. Despite the use of 9 amoeba supports for co-culture, most of the isolates were obtained from two amoebas: <i>Acanthamoeba castellanii</i> Neff and <i>Vermamoeba vermiformis</i> CDC 19. Finally, the viruses most frequently isolated were <i>marseilleviruses</i> (55.5%) and <i>Mimiviruses</i> (22.2%). This work shows that the isolation of viruses previously detected by metagenomic analyses can be tedious, but possible. |
| first_indexed | 2024-03-10T19:30:51Z |
| format | Article |
| id | doaj.art-98ba6d033e74459ea08735effa8a1766 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T19:30:51Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-98ba6d033e74459ea08735effa8a17662023-11-20T02:10:20ZengMDPI AGDiversity1424-28182020-05-0112621510.3390/d12060215Diversity of Amoeba-Associated Giant Viruses Isolated in AlgeriaHadjer Boudjemaa0Julien Andreani1Idir Bitam2Bernard La Scola3Department of Biology, Faculty of Science of Nature and Life, Hassiba Ben Bouali University of Chlef, 02180 Chlef, AlgeriaDepartment of Medicine, IHU-Méditerranée Infection, MEPHI, APHM, IRD 198, Aix Marseille Univ, 13007 Marseille, FranceLaboratoire Biodiversité-Environnement, France Ecole Supérieur en Sciences de l’Aliment et des Industries Agroalimentaires d’Alger, Université des Sciences et de la Technologie Houari Boumediene, 16111 Alger, AlgeriaDepartment of Medicine, IHU-Méditerranée Infection, MEPHI, APHM, IRD 198, Aix Marseille Univ, 13007 Marseille, FranceThe discovery of several giant amoeba viruses has opened up a novel area in the field of virology. Despite this, knowledge about ecology of these viruses remains patchy. In this study, we aimed to characterize the diversity of giant viruses in Algeria by inoculating 64 environmental samples on various amoeba strains. After isolation by co-culture with nine amoeba supports, flow cytometry and electron microscopy were used to putatively identify viruses. Definitive identification was performed by PCR and sequencing. Mimiviruses, marseilleviruses, faustoviruses and cedratviruses were the main viruses isolated in this study. Moreover, a new virus, which we named fadolivirus, was also isolated and was found to belong to the recent metagenomic descriptions of Klosneuvirinae. Despite the use of 9 amoeba supports for co-culture, most of the isolates were obtained from two amoebas: <i>Acanthamoeba castellanii</i> Neff and <i>Vermamoeba vermiformis</i> CDC 19. Finally, the viruses most frequently isolated were <i>marseilleviruses</i> (55.5%) and <i>Mimiviruses</i> (22.2%). This work shows that the isolation of viruses previously detected by metagenomic analyses can be tedious, but possible.https://www.mdpi.com/1424-2818/12/6/215Algeriaco-culturegiant virusescedratvirusmarseillevirusmimivirus |
| spellingShingle | Hadjer Boudjemaa Julien Andreani Idir Bitam Bernard La Scola Diversity of Amoeba-Associated Giant Viruses Isolated in Algeria Diversity Algeria co-culture giant viruses cedratvirus marseillevirus mimivirus |
| title | Diversity of Amoeba-Associated Giant Viruses Isolated in Algeria |
| title_full | Diversity of Amoeba-Associated Giant Viruses Isolated in Algeria |
| title_fullStr | Diversity of Amoeba-Associated Giant Viruses Isolated in Algeria |
| title_full_unstemmed | Diversity of Amoeba-Associated Giant Viruses Isolated in Algeria |
| title_short | Diversity of Amoeba-Associated Giant Viruses Isolated in Algeria |
| title_sort | diversity of amoeba associated giant viruses isolated in algeria |
| topic | Algeria co-culture giant viruses cedratvirus marseillevirus mimivirus |
| url | https://www.mdpi.com/1424-2818/12/6/215 |
| work_keys_str_mv | AT hadjerboudjemaa diversityofamoebaassociatedgiantvirusesisolatedinalgeria AT julienandreani diversityofamoebaassociatedgiantvirusesisolatedinalgeria AT idirbitam diversityofamoebaassociatedgiantvirusesisolatedinalgeria AT bernardlascola diversityofamoebaassociatedgiantvirusesisolatedinalgeria |