Evaluation of antibacterial activity of silver nanoparticles synthesized by a novel strain of marine Pseudomonas sp.
Use of silver nanoparticles to manage pathogenic microorganisms is a modern trend in nanomedicine. Thus our study focused on utilizing the nanoparticle synthesizing properties of marine bacteria. Molecular identification of the selected bacterial strain was done by 16SrDNA sequencing based method, w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2012-09-01
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| Series: | Nano Biomedicine and Engineering |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.5101/nbe.v4i3.p139-143 |
| _version_ | 1797673461892513792 |
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| author | Roshmi Thomas Aswathy Viswan Jyothis Mathew Radhakrishnan EK |
| author_facet | Roshmi Thomas Aswathy Viswan Jyothis Mathew Radhakrishnan EK |
| author_sort | Roshmi Thomas |
| collection | DOAJ |
| description | Use of silver nanoparticles to manage pathogenic microorganisms is a modern trend in nanomedicine. Thus our study focused on utilizing the nanoparticle synthesizing properties of marine bacteria. Molecular identification of the selected bacterial strain was done by 16SrDNA sequencing based method, which showed it as a novel Pseudomonas strain. The biosynthesis of silver nanoparticles was obtained by treating the bacteria with 1 mM AgNO3 and the isolate was found to have the ability to form silver nanoparticles intracellularly within 24 hours at room temperature. The silver nanoparticles synthesized by the novel isolate were characterized by UV-Vis spectroscopy and scanning electron microscope. The UV-Vis absorption analysis showed a peak at 430 nm corresponding to the surface plasmon resonance of silver nanoparticles. Also these silver nanoparticles were evaluated for their antibacterial efficacy against Salmonella typhi, Vibrio cholerae, Bacillus subtilis and Staphylococcus aureus. |
| first_indexed | 2024-03-11T21:45:45Z |
| format | Article |
| id | doaj.art-98bd4fca61254856be62f4d7024108bc |
| institution | Directory Open Access Journal |
| issn | 2150-5578 |
| language | English |
| last_indexed | 2024-03-11T21:45:45Z |
| publishDate | 2012-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj.art-98bd4fca61254856be62f4d7024108bc2023-09-26T10:12:42ZengTsinghua University PressNano Biomedicine and Engineering2150-55782012-09-014313914310.5101/nbe.v4i3.p139-143Evaluation of antibacterial activity of silver nanoparticles synthesized by a novel strain of marine Pseudomonas sp.Roshmi Thomas0Aswathy Viswan1Jyothis Mathew2Radhakrishnan EK3School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686560, IndiaSchool of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686560, IndiaSchool of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686560, IndiaSchool of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala, 686560, IndiaUse of silver nanoparticles to manage pathogenic microorganisms is a modern trend in nanomedicine. Thus our study focused on utilizing the nanoparticle synthesizing properties of marine bacteria. Molecular identification of the selected bacterial strain was done by 16SrDNA sequencing based method, which showed it as a novel Pseudomonas strain. The biosynthesis of silver nanoparticles was obtained by treating the bacteria with 1 mM AgNO3 and the isolate was found to have the ability to form silver nanoparticles intracellularly within 24 hours at room temperature. The silver nanoparticles synthesized by the novel isolate were characterized by UV-Vis spectroscopy and scanning electron microscope. The UV-Vis absorption analysis showed a peak at 430 nm corresponding to the surface plasmon resonance of silver nanoparticles. Also these silver nanoparticles were evaluated for their antibacterial efficacy against Salmonella typhi, Vibrio cholerae, Bacillus subtilis and Staphylococcus aureus.https://www.sciopen.com/article/10.5101/nbe.v4i3.p139-143silver nanoparticlesmarine pseudomonas sp.intracellularantibacterial activitypurification |
| spellingShingle | Roshmi Thomas Aswathy Viswan Jyothis Mathew Radhakrishnan EK Evaluation of antibacterial activity of silver nanoparticles synthesized by a novel strain of marine Pseudomonas sp. Nano Biomedicine and Engineering silver nanoparticles marine pseudomonas sp. intracellular antibacterial activity purification |
| title | Evaluation of antibacterial activity of silver nanoparticles synthesized by a novel strain of marine Pseudomonas sp. |
| title_full | Evaluation of antibacterial activity of silver nanoparticles synthesized by a novel strain of marine Pseudomonas sp. |
| title_fullStr | Evaluation of antibacterial activity of silver nanoparticles synthesized by a novel strain of marine Pseudomonas sp. |
| title_full_unstemmed | Evaluation of antibacterial activity of silver nanoparticles synthesized by a novel strain of marine Pseudomonas sp. |
| title_short | Evaluation of antibacterial activity of silver nanoparticles synthesized by a novel strain of marine Pseudomonas sp. |
| title_sort | evaluation of antibacterial activity of silver nanoparticles synthesized by a novel strain of marine pseudomonas sp |
| topic | silver nanoparticles marine pseudomonas sp. intracellular antibacterial activity purification |
| url | https://www.sciopen.com/article/10.5101/nbe.v4i3.p139-143 |
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