<i>Arcobacter butzleri</i> Biofilms: Insights into the Genes Beneath Their Formation
<i>Arcobacter butzleri</i>, the most prevalent species of the genus, has the demonstrated ability to adhere to various surfaces through biofilm production. The biofilm formation capability has been related to the expression of certain genes, which have not been characterized in <i>...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/7/1280 |
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| author | Adrián Salazar-Sánchez Itsaso Baztarrika Rodrigo Alonso Aurora Fernández-Astorga Ilargi Martínez-Ballesteros Irati Martinez-Malaxetxebarria |
| author_facet | Adrián Salazar-Sánchez Itsaso Baztarrika Rodrigo Alonso Aurora Fernández-Astorga Ilargi Martínez-Ballesteros Irati Martinez-Malaxetxebarria |
| author_sort | Adrián Salazar-Sánchez |
| collection | DOAJ |
| description | <i>Arcobacter butzleri</i>, the most prevalent species of the genus, has the demonstrated ability to adhere to various surfaces through biofilm production. The biofilm formation capability has been related to the expression of certain genes, which have not been characterized in <i>A. butzleri</i>. In order to increase the knowledge of this foodborne pathogen, the aim of this study was to assess the role of six biofilm-associated genes in campylobacteria (<i>flaA</i>, <i>flaB</i>, <i>fliS</i>, <i>luxS</i>, <i>pta</i> and <i>spoT</i>) in the biofilm formation ability of <i>A. butzleri</i>. Knockout mutants were constructed from different foodborne isolates, and static biofilm assays were conducted on polystyrene (PS), reinforced glass and stainless steel. Additionally, motility and Congo red binding assays were performed. In general, mutants in <i>flaAB</i>, <i>fliS</i> and <i>luxS</i> showed a decrease in the biofilm production irrespective of the surface; mutants in <i>spoT</i> showed an increase on stainless steel, and mutants in <i>pta</i> and <i>spoT</i> showed a decrease on reinforced glass but an increase on PS. Our work sheds light on the biofilm-related pathogenesis of <i>A. butzleri</i>, although future studies are necessary to achieve a satisfactory objective. |
| first_indexed | 2024-03-09T10:14:34Z |
| format | Article |
| id | doaj.art-98bf82f4e9cc4ee494f301e8d17f45d2 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T10:14:34Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-98bf82f4e9cc4ee494f301e8d17f45d22023-12-01T22:28:05ZengMDPI AGMicroorganisms2076-26072022-06-01107128010.3390/microorganisms10071280<i>Arcobacter butzleri</i> Biofilms: Insights into the Genes Beneath Their FormationAdrián Salazar-Sánchez0Itsaso Baztarrika1Rodrigo Alonso2Aurora Fernández-Astorga3Ilargi Martínez-Ballesteros4Irati Martinez-Malaxetxebarria5MikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain<i>Arcobacter butzleri</i>, the most prevalent species of the genus, has the demonstrated ability to adhere to various surfaces through biofilm production. The biofilm formation capability has been related to the expression of certain genes, which have not been characterized in <i>A. butzleri</i>. In order to increase the knowledge of this foodborne pathogen, the aim of this study was to assess the role of six biofilm-associated genes in campylobacteria (<i>flaA</i>, <i>flaB</i>, <i>fliS</i>, <i>luxS</i>, <i>pta</i> and <i>spoT</i>) in the biofilm formation ability of <i>A. butzleri</i>. Knockout mutants were constructed from different foodborne isolates, and static biofilm assays were conducted on polystyrene (PS), reinforced glass and stainless steel. Additionally, motility and Congo red binding assays were performed. In general, mutants in <i>flaAB</i>, <i>fliS</i> and <i>luxS</i> showed a decrease in the biofilm production irrespective of the surface; mutants in <i>spoT</i> showed an increase on stainless steel, and mutants in <i>pta</i> and <i>spoT</i> showed a decrease on reinforced glass but an increase on PS. Our work sheds light on the biofilm-related pathogenesis of <i>A. butzleri</i>, although future studies are necessary to achieve a satisfactory objective.https://www.mdpi.com/2076-2607/10/7/1280<i>Arcobacter butzleri</i>biofilmknockout mutantsCongo red assaystatic biofilm assays |
| spellingShingle | Adrián Salazar-Sánchez Itsaso Baztarrika Rodrigo Alonso Aurora Fernández-Astorga Ilargi Martínez-Ballesteros Irati Martinez-Malaxetxebarria <i>Arcobacter butzleri</i> Biofilms: Insights into the Genes Beneath Their Formation Microorganisms <i>Arcobacter butzleri</i> biofilm knockout mutants Congo red assay static biofilm assays |
| title | <i>Arcobacter butzleri</i> Biofilms: Insights into the Genes Beneath Their Formation |
| title_full | <i>Arcobacter butzleri</i> Biofilms: Insights into the Genes Beneath Their Formation |
| title_fullStr | <i>Arcobacter butzleri</i> Biofilms: Insights into the Genes Beneath Their Formation |
| title_full_unstemmed | <i>Arcobacter butzleri</i> Biofilms: Insights into the Genes Beneath Their Formation |
| title_short | <i>Arcobacter butzleri</i> Biofilms: Insights into the Genes Beneath Their Formation |
| title_sort | i arcobacter butzleri i biofilms insights into the genes beneath their formation |
| topic | <i>Arcobacter butzleri</i> biofilm knockout mutants Congo red assay static biofilm assays |
| url | https://www.mdpi.com/2076-2607/10/7/1280 |
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